Dynamic architecture of the Escherichia coli structural maintenance of chromosomes (SMC) complex, MukBEF. [PDF]
Nucleic Acids Res, 2019 Ubiquitous Structural Maintenance of Chromosomes (SMC) complexes use a proteinaceous ring-shaped architecture to organize and individualize chromosomes, thereby facilitating chromosome segregation.
Rajasekar KV +11 more
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MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation [PDF]
Nature Communications, 2016 MukBEF, the bacterial structural maintenance of chromosomes complex, is known to associate with origins of replication and topoisomerase IV. Here the authors show an association of MukBEF with MatP and replication termination regions, important for ...
Sophie Nolivos +9 more
doaj +7 more sources
A folded conformation of MukBEF and cohesin [PDF]
Nature Structural & Molecular Biology, 2019 Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, with key roles in chromosome segregation, DNA repair and regulation of gene expression.
Bürmann, F. +9 more
core +5 more sources
The SMC Complex MukBEF Recruits Topoisomerase IV to the Origin of Replication Region in Live
mBio, 2014 The Escherichia coli structural maintenance of chromosome (SMC) complex, MukBEF, and topoisomerase IV (TopoIV) interact in vitro through a direct contact between the MukB dimerization hinge and the C-terminal domain of ParC, the catalytic subunit of ...
Emilien Nicolas +5 more
doaj +6 more sources
The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF [PDF]
Cell Reports, 2015 The type II topoisomerase TopoIV, which has an essential role in Escherichia coli chromosome decatenation, interacts with MukBEF, an SMC (structural maintenance of chromosomes) complex that acts in chromosome segregation.
Pawel Zawadzki +6 more
doaj +3 more sources
Mid-cell migration of the chromosomal terminus is coupled to origin segregation in Escherichia coli. [PDF]
Nat Commun, 2023 Bacterial chromosomes are dynamically and spatially organised within cells. In slow-growing Escherichia coli, the chromosomal terminus is initially located at the new pole and must therefore migrate to midcell during replication to reproduce the same ...
Sadhir I, Murray SM.
europepmc +3 more sources
Intersubunit and intrasubunit interactions driving the MukBEF ATPase. [PDF]
J Biol Chem, 2022 MukBEF, a structural maintenance of chromosome-like protein complex consisting of an ATPase, MukB, and two interacting subunits, MukE and MukF, functions as the bacterial condensin. It is likely that MukBEF compacts DNA via an ATP hydrolysis-dependent DNA loop-extrusion reaction similar to that demonstrated for the yeast structural maintenance of ...
Bahng S, Kumar R, Marians KJ.
europepmc +4 more sources
Nonrandom segregation of sister chromosomes by <i>Escherichia coli</i> MukBEF. [PDF]
Proc Natl Acad Sci U S A, 2021 Significance Circular chromosomes in rod-shaped bacteria exist inside a cell in two distinct configurations, “transverse” and “longitudinal,” relative to the long cell axis, with chromosomal loci occupying specific cellular locations in both cases.
Mäkelä J, Uphoff S, Sherratt DJ.
europepmc +4 more sources
Physical models of bacterial chromosomes. [PDF]
Mol MicrobiolConnecting experimental observations to biophysical models can give rise to new insights on bacterial chromosome organization. Simple polymer models explore how physical constraints affect bacterial chromosome organization; bottom‐up mechanistic models connect these constraints to their underlying causes; and data‐driven models are inferred directly ...
Harju J, Broedersz CP.
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Cryo-EM structure of MukBEF reveals DNA loop entrapment at chromosomal unloading sites. [PDF]
Mol Cell, 2021 SummaryThe ring-like structural maintenance of chromosomes (SMC) complex MukBEF folds the genome of Escherichia coli and related bacteria into large loops, presumably by active DNA loop extrusion. MukBEF activity within the replication terminus macrodomain is suppressed by the sequence specific unloader MatP.
Bürmann F, Funke LFH, Chin JW, Löwe J.
europepmc +4 more sources