Results 21 to 30 of about 760 (134)

MukBEF-dependent chromosomal organization in widened Escherichia coli

, 2022
AbstractThe bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throughout all domains of life.
Japaridze A, van Wee R, Gogou C, Kerssemakers JWJ, Dekker C.   +4 more
europepmc   +2 more sources

MukBEF, a Chromosomal Organizer [PDF]

Microbial Physiology, 2014
Global folding of bacterial chromosome requires the activity of condensins. These highly conserved proteins are involved in various aspects of higher-order chromatin dynamics in a diverse range of organisms. Two distinct superfamilies of condensins have been identified in bacteria.
Valentin V, Rybenkov, Viridiana, Herrera, Zoya M, Petrushenko, Hang, Zhao   +3 more
openaire   +2 more sources

Organization of the Escherichia coli Chromosome by a MukBEF Axial Core. [PDF]

Mol Cell, 2020
Structural maintenance of chromosomes (SMC) complexes organize chromosomes ubiquitously, thereby contributing to their faithful segregation. We demonstrate that under conditions of increased chromosome occupancy of the Escherichia coli SMC complex, MukBEF, the chromosome is organized as a series of loops around a thin (
Mäkelä J, Sherratt DJ.
europepmc   +5 more sources

Mechanism of DNA capture by the MukBEF SMC complex and its inhibition by a viral DNA mimic. [PDF]

Cell
Ring-like structural maintenance of chromosome (SMC) complexes are crucial for genome organization and operate through mechanisms of DNA entrapment and loop extrusion. Here, we explore the DNA loading process of the bacterial SMC complex MukBEF. Using cryoelectron microscopy (cryo-EM), we demonstrate that ATP binding opens one of MukBEF's three ...
Bürmann F, Clifton B, Koekemoer S, Wilkinson OJ, Kimanius D, Dillingham MS, Löwe J.   +6 more
europepmc   +3 more sources

Characterization of the conserved features of the NSE6 subunit of the Physcomitrium patens SMC5/6 complex

The Plant Journal, Volume 115, Issue 4, Page 1084-1099, August 2023., 2023
Significance Statement The understanding of the role of the structural maintenance of chromosomes (SMC) 5/6 complexes in plants, particularly of the NSE5 and NSE6 subunits, is poor. We identified a previously unrecognized NSE6 motif mediating interaction with NSE5 and mapped the Physcomitrium patens PpNSE6‐binding site at the PpSMC5 arm. These findings
Edit Lelkes, Jitka Jemelková, Marcela Holá, Barbora Štefanovie, Peter Kolesár, Radka Vágnerová, Eva Dvořák Tomaštíková, Ales Pecinka, Karel J. Angelis, Jan J. Paleček   +9 more
wiley   +1 more source

Targeting Essential Hypothetical Proteins of Pseudomonas aeruginosa PAO1 for Mining of Novel Therapeutics: An In Silico Approach

BioMed Research International, Volume 2023, Issue 1, 2023., 2023
As an omnipresent opportunistic bacterium, Pseudomonas aeruginosa PAO1 is responsible for acute and chronic infection in immunocompromised individuals. Currently, this bacterium is on WHO’s red list where new antibiotics are urgently required for the treatment.
Atikur Rahman, Md. Takim Sarker, Md Ashiqul Islam, Mohammad Uzzal Hossain, Mahmudul Hasan, Tasmina Ferdous Susmi, Luis Morales-Quintana   +6 more
wiley   +1 more source

The Role of MukE in Assembling a Functional MukBEF Complex [PDF]

Journal of Molecular Biology, 2011
The MukB-MukE-MukF protein complex is essential for chromosome condensation and segregation in Escherichia coli. The central component of this complex, the MukB protein, is related functionally and structurally to the ubiquitous SMC (structural maintenance of chromosomes) proteins.
Melanie, Gloyd, Rodolfo, Ghirlando, Alba, Guarné   +2 more
openaire   +2 more sources

Non-random segregation of sister chromosomes byEscherichia coliMukBEF axial cores [PDF]

SSRN Electronic Journal, 2020
SummaryTheEscherichia colistructural maintenance of chromosomes complex, MukBEF, forms axial cores to chromosomes that determine their spatio-temporal organization. Here, we show that axial cores direct chromosome arms to opposite poles and generate the translational symmetry between newly replicated sister chromosomes.
Jarno Mäkelä, Stephan Uphoff, David J. Sherratt   +2 more
openaire   +2 more sources

Nucleoid‐associated Rok differentially affects chromosomal transformation on Bacillus subtilis recombination‐deficient cells

Environmental Microbiology, Volume 23, Issue 6, Page 3318-3331, June 2021., 2021
Summary Rok, a Bacillus subtilis nucleoid‐associated protein (NAP), negatively regulates competence development and silences xenogeneic genes. We show that rok inactivation increases rpoB482 natural intraspecies chromosomal transformation (CT) and plasmid transformation to a different extent.
Ester Serrano, Rubén Torres, Juan C. Alonso   +2 more
wiley   +1 more source

Cancer‐associated mutations in the condensin II subunit CAPH2 cause genomic instability through telomere dysfunction and anaphase chromosome bridges

Journal of Cellular Physiology, Volume 236, Issue 5, Page 3579-3598, May 2021., 2021
The condensin II complex mutations R551P, R551S, and S556F cause genomic instability by causing DNA damage, anaphase defects, micronuclei, and chromosomal instability. DNA damage and anaphase defects are caused primarily by ataxia telangiectasia and Rad3‐related‐dependent telomere dysfunction.
Emily Weyburne, Giovanni Bosco
wiley   +1 more source