Cell boundary confinement sets the size and position of the E. coli chromosome [PDF]
, 2019 Although the spatiotemporal structure of the genome is crucial to its biological function, many basic questions remain unanswered on the morphology and segregation of chromosomes.
Chaudhuri, Debasish +11 more
core +2 more sources
MukB ATPases are regulated independently by the N- and C-terminal domains of MukF kleisin
eLife, 2018 The Escherichia coli SMC complex, MukBEF, acts in chromosome segregation. MukBEF shares the distinctive architecture of other SMC complexes, with one prominent difference; unlike other kleisins, MukF forms dimers through its N-terminal domain.
Katarzyna Zawadzka +6 more
doaj +1 more source
Are SMC complexes loop extruding factors? Linking theory with fact [PDF]
, 2019 The extreme length of chromosomal DNA requires organizing mechanisms to both promote functional genetic interactions and ensure faithful chromosome segregation when cells divide.
Akai +102 more
core +1 more source
The Escherichia coli SMC Complex, MukBEF, Shapes Nucleoid Organization Independently of DNA Replication [PDF]
Journal of Bacteriology, 2012 ABSTRACTSMC (structuralmaintenance ofchromosomes) complexes function ubiquitously in organizing and maintaining chromosomes. Functional fluorescent derivatives of theEscherichia coliSMC complex, MukBEF, form foci that associate with the replication origin region (ori).
Badrinarayanan, A +3 more
openaire +3 more sources
Molecular Microbiology, Volume 114, Issue 5, Page 839-856, November 2020., 2020 The rod‐shaped Myxococcus xanthus cells have a unique chromosome organization whereby the chromosome occupies the central part of cells leaving large subpolar regions devoid of DNA. We show that three systems contribute to chromosome segregation and organization in M.
Deepak Anand +2 more
wiley +1 more source
Intermittent transport of bacterial chromosomal loci [PDF]
, 2017 The short-time dynamics of bacterial chromosomal loci is a mixture of subdiffusive and active motion, in the form of rapid relocations with near-ballistic dynamics.
Calabrese, Ludovico +3 more
core +2 more sources
Determining cellular CTCF and cohesin abundances to constrain 3D genome models. [PDF]
, 2019 Achieving a quantitative and predictive understanding of 3D genome architecture remains a major challenge, as it requires quantitative measurements of the key proteins involved.
Cattoglio, Claudia +11 more
core +2 more sources
Focal localization of MukBEF condensin on the chromosome requires the flexible linker region of MukF [PDF]
The FEBS Journal, 2009 Condensin complexes are the key mediators of chromosome condensation. The MukB–MukE–MukF complex is a bacterial condensin, in which the MukB subunit forms a V‐shaped dimeric structure with two ATPase head domains. MukE and MukF together form a tight complex, which binds to the MukB head via the C‐terminal winged‐helix domain (C‐WHD) of MukF. One of the
Shin, HC, Lim, JH, Woo, JS, Oh, BH
openaire +3 more sources
Comparative genomics of Burkholderia multivorans, a ubiquitous pathogen with a highly conserved genomic structure [PDF]
, 2017 The natural environment serves as a reservoir of opportunistic pathogens. A well-established method for studying the epidemiology of such opportunists is multilocus sequence typing, which in many cases has defined strains predisposed to causing infection.
Peeters, Charlotte +5 more
core +3 more sources
Bacillus Subtilis SMC Complexes Juxtapose Chromosome Arms as They Travel from Origin to Terminus [PDF]
, 2017 Structural maintenance of chromosomes (SMC) complexes play critical roles in chromosome dynamics in virtually all organisms, but how they function remains poorly understood.
Brandão, Hugo B. +4 more
core +1 more source