Results 101 to 110 of about 32,835 (232)

Expanding the Toolbox for Biocatalytic Halogenation by Identification and Characterization of Three Vanadium Chloroperoxidases

ChemBioChem, Volume 27, Issue 11, 15 June 2026.
Schematic illustration of the workflow from DNA sequence‐based research to expression in shaking flasks and subsequent purification via IMAC. The obtained enzymes broaden the toolbox of enzymes used for halogenation reactions with differing catalytic properties and tolerances to reaction‐related conditions.
Sonja Schönrock, Nils Senge, Dirk Holtmann   +2 more
wiley   +1 more source

Enhanced Human Antigen‐Specific B Cell Responses Using In Vitro 3D Tonsil Cultures Containing Stromal Cells

Advanced Healthcare Materials, Volume 15, Issue 22, 12 June 2026.
We developed a fully human 3D tonsil cell culture system incorporating supportive stromal cells that better sustains and activates immune cells than conventional methods. The model generates stronger, more targeted antibody responses to viral antigens and vaccines, providing a physiologically relevant and entirely human platform for studying immune ...
Maaike V. J. Braham, Marlon de Gast, Liubov Babii, Sabine Kruijer, Theo M. Bestebroer, Mathilde Richard, Mathieu Claireaux, S. Marieke van Ham, Jelle de Wit, Cécile A. C. M. van Els, Anja ten Brinke, Marit J. van Gils   +11 more
wiley   +1 more source

Integrase-RNA interactions underscore the critical role of integrase in HIV-1 virion morphogenesis

eLife, 2020
A large number of human immunodeficiency virus 1 (HIV-1) integrase (IN) alterations, referred to as class II substitutions, exhibit pleiotropic effects during virus replication.
Jennifer L Elliott, Jenna E Eschbach, Pratibha C Koneru, Wen Li, Maritza Puray-Chavez, Dana Townsend, Dana Q Lawson, Alan N Engelman, Mamuka Kvaratskhelia, Sebla B Kutluay   +9 more
doaj   +1 more source

A quantitative IN subunit exchange assay specifically illustrates multimerization defect of IN dimeric interface mutants.

, 2013
(A) Mechanics of the subunit exchange assay for quantifying HIV-1 IN multimerization. Mutant IN was expressed with a 6x Histidine tag, while WT IN was expressed with a GST tag.
Wannes Thys (307258), Jonas Demeulemeester (180282), Zeger Debyser (20227), Laith Q. Al-Mawsawi (307260), Erik Serrao (307256), Frauke Christ (180292), Nouri Neamati (275633)   +6 more
core   +1 more source

Hierarchically Engineered Multi‐Enzyme Nanoreactors for in vitro Drug Biosynthesis and Pathway Transplantation Into Cells

Advanced Materials, Volume 38, Issue 34, 17 June 2026.
A hierarchically structured metal–organic framework (eMIL) is shown to host the complete six‐enzyme biosynthesis pathway for the anti‐cancer pigment violacein, reshaping reaction dynamics and multiplying in vitro yields. eMIL nanoreactors deliver this reconstituted bacterial multi‐protein system into mammalian cells, where cell‐derived substrates and ...
Ainur Sharip, Somayah S. Qutub, Manar M. Farooqui, Walaa Baslyman, Nida Khalfay, Lukman O. Alimi, Patricia Lopez Sanchez, Lingyun Zhao, Milena Chernyshevskaia, Giovanni Colombo, Niveen M. Khashab, Stefan T. Arold, Raik Grünberg   +12 more
wiley   +1 more source

ROS responsive Aux/IAA multimerization modulates auxin responses

Reactive oxygen species (ROS) function as key signals in plants to enable adaptation to environmental stresses. Plant roots respond to transient water stress by temporarily ceasing branching using the acclimative response xerobranching 1 . In this study,
Mullineaux, Phil, Lilley, Kathryn, Sadanandom, Ari, Mehra, Poonam, Brown, Adrian, Bellande, Kevin, Bechtold, Ulrike, Clark, Lisa, Mukkawar, Vaishnavi, Roy, Dipan, Vermeer, Joop Em, Vernoux, Teva, Arevallilo, Raquel Martin, Bennett, Malcolm, Jones, Andrew, Sue Ob, Kawinnat   +15 more
core   +1 more source

The Emerging Parkinson's Disease Oxylipin‐Ome

Advanced Science, Volume 13, Issue 33, 15 June 2026.
ABSTRACT Parkinson Disease (PD) is increasingly considered a proteinopathy and lipidopathy. This proteinopathy+lipidopathy paradigm has been further refined to a fatty acid (FA)‐opathy, centering dysregulated FA metabolism as fundamental in PD lipid dysfunction.
Julia C. Kelliher, Saranna Fanning
wiley   +1 more source

Functional multimerization of mucolipin channel proteins [PDF]

, 2010
MCOLN1 encodes mucolipin-1 (TRPML1), a member of the transient receptor potential TRPML subfamily of channel proteins. Mutations in MCOLN1 cause mucolipidosis-type IV (MLIV), a lysosomal storage disorder characterized by severe neurologic, ophthalmologic,
Zhang, Peng, Curcio Morelli, Cyntia, Cantiello, Horacio Fabio, Browning, Marsha F., Venugopal, Bhuvarahamurthy, Slaugenhaupt, Susan A., Charles, Florie A.   +6 more
core   +1 more source

A common allosteric mechanism regulates homeostatic inactivation of auxin and gibberellin

Nature Communications, 2020
Enzymatic inactivation of gibberellins and auxin, via GA2ox3 and DAO respectively, contributes to hormone homeostasis in plants. Here Takehara et al.
Sayaka Takehara, Shun Sakuraba, Bunzo Mikami, Hideki Yoshida, Hisako Yoshimura, Aya Itoh, Masaki Endo, Nobuhisa Watanabe, Takayuki Nagae, Makoto Matsuoka, Miyako Ueguchi-Tanaka   +10 more
doaj   +1 more source

High‐Yield Production and Cost‐Effective Purification of an Alkali‐Resistant Hexameric Protein A Variant for Antibody Affinity Chromatography

Biotechnology Journal, Volume 21, Issue 6, June 2026.
Graphical Abstract and Lay Summary We present a scalable method for producing the antibody‐binding ligand Z6AlkC, a six‐repeat, alkali‐resistant Protein A–derived Z domain with a terminal cysteine. The ligand was produced at high yield via extracellular secretion in bioreactors and subsequently purified by high‐productivity ultradiafiltration with low ...
Carolina Mota Natal, Claudia Lacombe, Alexander Zollner, Catarina Domingos, José Mendes, André Nascimento, Ana Margarida Gonçalves Carvalho Dias, Arménio Jorge Moura Barbosa, Cristina Peixoto, Birgit Wiltschi, Alois Jungbauer, Ana Cecília Afonso Roque   +11 more
wiley   +1 more source