Results 31 to 40 of about 38,467 (245)

Protein Condensate Formation via Controlled Multimerization of Intrinsically Disordered Sequences

Biochemistry, 2022
Proteins harboring low complexity or intrinsically disordered sequences (IDRs) are capable of undergoing liquid-liquid phase separation to form mesoscale condensates that function as biochemical niches with the ability to concentrate or sequester ...
Mikael V. Garabedian, Zhihui Su, Jorge B. Dabdoub, Michelle Tong, A. Deiters, D. Hammer, M. Good   +6 more
semanticscholar   +1 more source

A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses

Nature Communications, 2020
There is need for effective and affordable vaccines against SARS-CoV-2 to tackle the ongoing pandemic. In this study, we describe a protein nanoparticle vaccine against SARS-CoV-2.
T. Tan, P. Rijal, Rolle Rahikainen, A. H. Keeble, L. Schimanski, S. Hussain, R. Harvey, Jack W. P. Hayes, J. Edwards, R. McLean, Veronica Martini, M. Pedrera, Nazia Thakur, Carina Conceicao, Isabelle Dietrich, Holly Shelton, A. Ludi, G. Wilsden, Clare F. J. Browning, Adrian K. Zagrajek, D. Bialy, Sushant Bhat, Phoebe Stevenson-Leggett, Philippa Hollinghurst, Matthew Tully, Katy Moffat, Chris Chiu, R. Waters, Ashley R. Gray, Mehreen Azhar, V. Mioulet, J. Newman, A. Asfor, Alison Burman, S. Crossley, J. Hammond, E. Tchilian, B. Charleston, D. Bailey, T. Tuthill, S. Graham, Helen M. E. Duyvesteyn, T. Malinauskas, J. Huo, J. Tree, K. Buttigieg, R. Owens, M. Carroll, R. Daniels, J. McCauley, David I. Stuart, Kuan-Ying A. Huang, M. Howarth, A. Townsend   +53 more
semanticscholar   +1 more source

Increase in cysteine-mediated multimerization under attractive protein–protein interactions

, 2022
The CASPON enzyme became an interesting enzyme for fusion protein processing because it generates an authentic N-terminus. However, the high cysteine content of the CASPON enzyme may induce aggregation via disulfide-bond formation, which can reduce ...
Tomás Mesurado (14306958), Alois Jungbauer (1803292), Leo A. Jakob (13240167), Nico Lingg (14306961)   +3 more
core   +1 more source

Plasma Membrane Anchoring and Gag:Gag Multimerization on Viral RNA Are Critical Properties of HIV-1 Gag Required To Mediate Efficient Genome Packaging

mBio, 2021
To generate infectious virions, HIV-1 must package its full-length RNA as the genome during particle assembly. HIV-1 Gag:RNA interactions mediate genome packaging, but the mechanism remains unclear.
Alice Duchon, Steven Santos, Jianbo Chen, Matthew Brown, Olga A. Nikolaitchik, Sheldon Tai, Jeffrey A. Chao, E. Freed, V. Pathak, Wei-Shau Hu   +9 more
semanticscholar   +1 more source

Efficient Delivery of Macromolecules into Human Cells by Improving the Endosomal Escape Activity of Cell-Penetrating Peptides: Lessons Learned from dfTAT and its Analogs

Biomolecules, 2018
Cell-penetrating peptides (CPPs) are typically prone to endocytic uptake into human cells. However, they are often inefficient at escaping from endosomes, which limits their ability to deliver cargos into cells.
Jason K. Allen, Dakota J. Brock, Helena M. Kondow-McConaghy, Jean-Philippe Pellois   +3 more
doaj   +1 more source

Targeting GD2-Positive Tumor Cells by Pegylated scFv Fragment–Drug Conjugates Carrying Maytansinoids DM1 and DM4

Current Issues in Molecular Biology, 2023
Oligomerization of antibody fragments via modification with polyethylene glycol (pegylation) may alter their function and properties, leading to a multivalent interaction of the resulting constructs with the target antigen.
Daniel V. Kalinovsky, Irina V. Kholodenko, Elena V. Svirshchevskaya, Alexey V. Kibardin, Dmitry Yu. Ryazantsev, Fedor N. Rozov, Sergey S. Larin, Sergey M. Deyev, Roman V. Kholodenko   +8 more
doaj   +1 more source

Pretargeted Imaging with Gallium-68—Improving the Binding Capability by Increasing the Number of Tetrazine Motifs

Pharmaceuticals, 2018
The inverse electron-demand Diels-Alder reaction between 1,2,4,5-tetrazine (Tz) and trans-cyclooct-2-ene (TCO) has gained increasing attraction among extensive studies on click chemistry due to its exceptionally fast reaction kinetics and high ...
Dominik Summer, Sonja Mayr, Milos Petrik, Christine Rangger, Katia Schoeler, Lisa Vieider, Barbara Matuszczak, Clemens Decristoforo   +7 more
doaj   +1 more source

Multimerization variants as potential drivers of neofunctionalization

Science Advances, 2021
A high-throughput analysis of protein complex variants among orthologs provides a mechanistic model for neofunctionalization. Whole-genome duplications are common during evolution, creating genetic redundancy that can enable cellular innovations.
Youngwoo Lee, D. Szymanski
semanticscholar   +1 more source

New Insights Into Retroviral Gag-Gag and Gag-Membrane Interactions

Frontiers in Microbiology, 2014
A critical aspect of viral replication is the assembly of virus particles, which aresubsequently released as progeny virus. While a great deal of attention has beenfocused on better understanding this phase of the viral life cycle, many aspects of ...
Jose eMaldonado, Jessica eMartin, Joachim eMueller, Wei eZhang, Louis eMansky   +4 more
doaj   +1 more source

Multi-Substituted Quinolines as HIV-1 Integrase Allosteric Inhibitors

Viruses, 2022
Allosteric HIV-1 integrase (IN) inhibitors, or ALLINIs, are a new class of antiviral agents that bind at the dimer interface of the IN, away from the enzymatic catalytic site and block viral replication by triggering an aberrant multimerization of the ...
Long Phi Dinh, Jian Sun, Courtney D. Glenn, Krunal Patel, Julie A. Pigza, Matthew G. Donahue, Larry Yet, Jacques J. Kessl   +7 more
doaj   +1 more source