Results 91 to 100 of about 279,791 (361)

Programmable DNA‐Peptide Hybrid Nanostructures for Potent Neutralization of Multiple Influenza a Virus Subtypes

Advanced Functional Materials, EarlyView.
A multivalent antiviral platform based on honeycomb‐shaped DNA nanostructures (HC–Urumin) is developed to enhance the potency and breadth of the host defense peptide Urumin. Through spatially patterned trimeric presentation, HC–Urumin disrupts influenza A virus entry, improves cell viability, and reduces disease severity in vivo‐offering a modular and ...
Saurabh Umrao, Abhisek Dwivedy, Payton L. Haak, Dhanush Gandavadi, Laurie A. Rund, Chi Chen, Vineetha Mareddy, Lifeng Zhou, Jinwei Duan, Ying Fang, Andrew J. Steelman, Xing Wang   +11 more
wiley   +1 more source

Development of Multiplex PCR for Simultaneous Detection of Trypanosoma evansi and Equine Piroplasma Infection in Horses

Hayati Journal of Biosciences
The identification of equine piroplasmosis and surra disease in infected horses currently presents a significant challenge. Clinical symptoms and blood smears are commonly used to diagnose these two diseases. The objective of this study was to develop a
Arifin Nugraha, Umi Cahyaningsih
doaj   +1 more source

Multiplex PCR theranostics of severe respiratory infections [PDF]

Expert Review of Anti-infective Therapy, 2010
Acute respiratory tract infection is the lead-ing cause of hospitalization for infants and young children in developed countries, and a major determinant of death in develop-ing countries. In 2002, lower respiratory tract infections accounted for approxi-mately 4 million deaths worldwide and 7% of all deaths that ...
Pozzetto, Bruno, Grattard, Florence, Pillet, Sylvie   +2 more
openaire   +4 more sources

VIVID: A qPCR‐Based Platform for Sensitive and Quantitative In Vivo Tracking of Extracellular Vesicles

Advanced Functional Materials, EarlyView.
This study introduces VIVID (Vesicle In Vivo Identification using DNA), a qPCR‐based platform that tracks PCR‐amplifiable DNA tags loaded in the EVs for accurate and quantifiable EV biodistribution in vivo. ABSTRACT Extracellular vesicles (EVs) represent promising carriers for nucleic acid therapeutics, offering advantages over synthetic nanoparticles ...
Oscar Boyadjian, Talin Ebrahimian, Jonathan Miranda O'Connor, Janusz Rak, Stephanie Lehoux, Maryam Tabrizian   +5 more
wiley   +1 more source

Identification of respiratory virus in infants with congenital heart disease by comparison of different methods

Revista do Instituto de Medicina Tropical de São Paulo, 2011
Respiratory virus infections are the main cause of infant hospitalization and are potentially severe in children with congenital heart disease (CHD).
Tatiana Mitiko Kanashiro, Lucy Santos Vilas Boas, Ana Maria Thomaz, Tania Regina Tozetto-Mendoza, Mônica Setsuko, Clarisse Martins Machado   +5 more
doaj   +1 more source

Validation and implementation of a diagnostic algorithm for DNA Detection of Bordetella pertussis, B. parapertussis, and B-holmesii in a Pediatric Referral Hospital in Barcelona, Spain [PDF]

, 2019
This study aimed to validate a comprehensive diagnostic protocolbased on real-time PCR for the rapid detection and identification ofBordetella per-tussis,Bordetella parapertussis, andBordetella holmesii, as well as its implementationin the diagnostic ...
Acosta Argueta, Lesly María, Godoy Garcia, Pere, Henares Bonilla, Desirée, Jané Checa, Mireia, Muñoz Almagro, Carmen, Valero Rello, Anna   +5 more
core   +2 more sources

Microfluidic Modeling of Macrophage‐Induced Cardiac Inflammation Using NF‐κB Reporter Cardiomyocytes

Advanced Healthcare Materials, EarlyView.
A cardiac inflammation model was developed to enable real‐time analysis of macrophage‐cardiomyocyte interactions using NF‐κB‐reporter cardiomyocytes and a microfluidic co‐culture system. A MATLAB‐based model was implemented to optimize cell density and medium resupply based on TNFα diffusion kinetics. Activation thresholds and stress responses in NF‐κB‐
Chao Liu, Xi Rui Qiang Lu, Seoyoung Jeong, Douglas H. Wu, Rodrigo A. Somoza, Parameswaran Ramakrishnan, Samuel E. Senyo   +6 more
wiley   +1 more source

STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods [PDF]

, 2017
The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis.
Cornelis, Senne, Deforce, Dieter, Deleye, Lieselot, Tilleman, Laurentijn, Van Nieuwerburgh, Filip, Vander Plaetsen, Ann-Sophie   +5 more
core   +2 more sources

Spherical Skin Model: Stratified Co‐Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening

Advanced Healthcare Materials, EarlyView.
Models of the human skin must combine the relevant biological contents and suitable biomaterials with the correct spatial organization. Performing compound screening on such in vitro models also requires fast and reproducible production methods of the models.
Elisa Lenzi, Nadia Vertti‐Quintero, Julien Husson, Anne‐Laure Bulteau, Carine Nizard, Karl Pays, Sébastien Sart, Charles N. Baroud   +7 more
wiley   +1 more source

Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR

Indonesian Journal of Biotechnology, 2006
world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detecting dengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primers that conserve for several ...
Nastiti Wijayanti, Hera Nirwati, Tri Wibawa, Aris Haryanto, S. Sutaryo   +4 more
doaj   +1 more source