Results 41 to 50 of about 279,791 (361)
Forensic SNP genotyping using nanopore MinION sequencing [PDF]
, 2017 One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using
Cornelis, Senne +4 more
core +2 more sources
Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source
BMC Microbiology, 2019 Background Acinetobacter baumannii is a cosmopolitan bacterium that is frequently reported from hospitalized patients, especially those patients who admitted in the intensive care unit.
Hossein Goudarzi +5 more
doaj +1 more source
Multiplexed Real-Time PCR Using Universal Reporters [PDF]
Clinical Chemistry, 2004 Real-time quantitative PCR is a sensitive and accurate method for gene expression studies (1). The detection chemistries of all real-time PCR procedures are based on one of two principles for monitoring amplification products: binding to double-stranded DNA or hybridization to single-stranded DNA.
Rickert, A., Lehrach, H., Sperling, S.
openaire +3 more sources
Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions
FEBS Letters, EarlyView.Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf +6 more
wiley +1 more source
Establishment of multiplex PCR for detection of genes related to Quinolone resistance
Vietnam Journal of Science, Technology and Engineering, 2018 Background/aims: It has been shown that quinolone resistance arises due to mutations in the quinolone resistance-determining regions of the drug targets.
Ngo Tat Trung +4 more
doaj +1 more source
Diagnostic Accuracy of Multiplex Polymerase Chain Reaction in Early Onset Neonatal Sepsis
Children, 2023 Early onset neonatal sepsis is a significant contributor to neonatal morbidity and mortality. Although blood cultures remain the diagnostic gold standard, they detect pathogens in only a minority of suspected cases.
Anja Stein +3 more
doaj +1 more source
Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]
, 2019 Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan +4 more
core +1 more source
FEBS Letters, EarlyView.Bone metastasis in prostate cancer (PCa) patients is a clinical hurdle due to the poor understanding of the supportive bone microenvironment. Here, we identify stearoyl‐CoA desaturase (SCD) as a tumor‐promoting enzyme and potential therapeutic target in bone metastatic PCa.
Alexis Wilson +7 more
wiley +1 more source
Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus [PDF]
Veterinaria Italiana, 2014 Abortion among dairy cattle is one of the major causes of economic losses in the livestock industry. This study describes a 1-step multiplex real-time polymerase chain reaction (PCR) to detect Brucella spp., Leptospira spp.
Abdelfattah M. Selim +2 more
doaj +1 more source