Results 61 to 70 of about 281,013 (338)

Highly multiplexed single-cell quantitative PCR

PLOS ONE, 2018
We present a microfluidic device for rapid gene expression profiling in single cells using multiplexed quantitative polymerase chain reaction (qPCR). This device integrates all processing steps, including cell isolation and lysis, complementary DNA synthesis, pre-amplification, sample splitting, and measurement in twenty separate qPCR reactions.
Michael VanInsberghe, Hans Zahn, Adam K White, Oleh I Petriv, Carl L Hansen   +4 more
openaire   +4 more sources

Integrated genomic and proteomic profiling reveals insights into chemoradiation resistance in cervical cancer

Molecular Oncology, EarlyView.
A comprehensive genomic and proteomic analysis of cervical cancer revealed STK11 and STX3 as a potential biomarkers of chemoradiation resistance. Our study demonstrated EGFR as a therapeutic target, paving the way for precision strategies to overcome treatment failure and the DNA repair pathway as a critical mechanism of resistance.
Janani Sambath, Irene A. George, Srikanth S. Manda, Prasanth Ariyannur, Ekta R. Dhawale, Raja Sekhar Kommu, Rajan Datar, Darshana Patil, Vinita Trivedi, Manisha Singh, Kumar Prabhash, Sewanti Limaye, Richa Chauhan, Prashant Kumar   +13 more
wiley   +1 more source

Identification and Discrimination of Salmonella enterica Serovar Gallinarum Biovars Pullorum and Gallinarum Based on a One-Step Multiplex PCR Assay

Frontiers in Microbiology, 2018
Salmonella enterica serovar Gallinarum biovars Pullorum (S. Pullorum) and Gallinarum (S. Gallinarum) can result in pullorum disease and fowl typhoid in avian species, respectively, and cause considerable economic losses in poultry in many developing ...
Dan Xiong, Dan Xiong, Dan Xiong, Dan Xiong, Li Song, Li Song, Li Song, Li Song, Zhiming Pan, Zhiming Pan, Zhiming Pan, Zhiming Pan, Xinan Jiao, Xinan Jiao, Xinan Jiao, Xinan Jiao   +15 more
doaj   +1 more source

Kesesuaian Uji Antigen Capture Enzyme Linked Immunosorbant Assay dan Nested Multiplex Polymerase Chain Reaction untuk Diagnosis Rutin Bovine Viral Diarrhea

Jurnal Ilmu Peternakan dan Veteriner Tropis, 2022
Bovine Viral Diarrhea (BVD) is one of the main causes of impaired productivity and reproduction of cows. Antigen capture Elisa (ACE) is one of the serological technique that is sensitive, reliable and used regularly for detecting persistent BVD infection
Sri Handayani Irianingsih, Dessie Eri Waluyati, Desi Puspita Sari, Hastari Wuryastuty   +3 more
doaj   +1 more source

Rapid Detection of Respiratory Pathogens Using a Multiplex PCR Assay Among Hospitalized Children with Acute Respiratory Infection [PDF]

, 2021
Katherine Javier, Josephine Anne Navoa-Ng, Nikki Cotoco-Chu   +2 more
openalex   +1 more source

Feasibility of a ctDNA multigenic panel for non‐small‐cell lung cancer early detection and disease surveillance

Molecular Oncology, EarlyView.
Plasma‐based detection of actionable mutations is a promising approach in lung cancer management. Analysis of ctDNA with a multigene NGS panel identified TP53, KRAS, and EGFR as the most frequently altered, with TP53 and KRAS in treatment‐naïve patients and TP53 and EGFR in previously treated patients.
Giovanna Maria Stanfoca Casagrande, Marcela de Oliveira Silva, Mariana Bisarro dos Reis, Rodrigo de Oliveira Cavagna, Luciane Sussuchi, Icaro Alves Pinto, Natalia Zampieri Pontes, Rodrigo Sampaio Chiarantano, Flavio Augusto Ferreira da Silva, Pedro de Marchi, Letícia Ferro Leal, Rui M. Reis   +11 more
wiley   +1 more source

Simplified development of multiplex real-time PCR through master mix augmented by universal fluorogenic reporters

BioTechniques, 2016
Mediator probe (MP) PCR is a real-time PCR approach that uses standardized universal fluorogenic reporter oligonucleotides (UR) in conjunction with label-free sequence-specific probes.
Simon Wadle, Michael Lehnert, Friedrich Schuler, René Köppel, Annerose Serr, Roland Zengerle, Felix von Stetten   +6 more
doaj   +1 more source

Digital PCR as a tool to measure HIV persistence [PDF]

, 2018
Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped.
Bosman, Kobus, Nijhuis, Monique, Rutsaert, Sofie, Trypsteen, Wim, Vandekerckhove, Linos   +4 more
core   +1 more source

Fundamentals of multiplexing with digital PCR

Biomolecular Detection and Quantification, 2016
Over the past decade numerous publications have demonstrated how digital PCR (dPCR) enables precise and sensitive quantification of nucleic acids in a wide range of applications in both healthcare and environmental analysis. This has occurred in parallel with the advances in partitioning fluidics that enable a reaction to be subdivided into an ...
Whale, Alexandra S., Huggett, Jim F., Tzonev, Svilen   +2 more
openaire   +2 more sources

KIR Genotyping by Multiplex PCR-SSP [PDF]

, 2009
Diversity across KIR haplotypes stems from differences in numbers of inhibitory and activating receptors, as well as allelic polymorphism of individual genes. The KIR locus has undergone large expansions and contractions over time and is believed to be coevolving with genes encoding its HLA class I ligands located within the MHC locus.
Smita, Kulkarni, Maureen P, Martin, Mary, Carrington   +2 more
openaire   +2 more sources