Results 91 to 100 of about 465,638 (353)

Ribosome Homeostasis Regulated by SETD2 Preserves Intestinal Epithelial Barrier

Advanced Science, EarlyView.
SETD2 ablation causes dysregulation and recruitment defects of ribosome biogenesis factors, resulting in translational disorders of barrier maintenance genes, thereby compromising the intestinal barrier. These findings unveil a previously unappreciated role of ribosome biogenesis and translational regulation in preserving the intestinal epithelial ...
Hanyu Rao, Aiting Wang, Yue Xu, Wenxin Feng, Chunxiao Ma, Ziyi Wang, Wei Zhang, Wenqiong Su, Xiuying Xiao, Wei‐Qiang Gao, Xianting Ding, Li Li   +11 more
wiley   +1 more source

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

Single‐Cell Dissection Reveals Immune Dysregulation After CD5 or CD7‐Directed Chimeric Antigen Receptor T‐Cell Therapy

Advanced Science, EarlyView.
Single‐cell RNA/TCR/BCR sequencing reveals that 5CAR therapy in T‐ALL induces T‐cell exhaustion, reduces EBV‐associated TCRs, lowers TCR/BCR diversity, and increases NK/DC/monocyte activation and function. In contrast, 7CAR therapy reduces multiple pathogen‐associated TCRs, enhances NK cell activation and function, decreases monocyte activation, and ...
Yuechen Luo, Haixiao Zhang, Kaiting Tang, Yiming Wang, Huajiang Dong, Wei Qi, Lingling Shan, Yue Tan, Liping Zhao, Jun Shi, Erlie Jiang, Jing Pan, Xiaoming Feng   +12 more
wiley   +1 more source

Comparison of prevalence estimates of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum determined by conventional PCR and multiplex qPCR and implications for surveillance and monitoring

International Journal of Infectious Diseases
Objectives: The accuracy of malaria rapid diagnostic tests is threatened by Plasmodium falciparum with pfhrp2/3 deletions. This study compares gene deletion prevalence determined by multiplex real time polymerase chain reaction (qPCR) and conventional ...
Michelle L. Gatton, David Smith, Cielo Pasay, Karen Anderson, Selam Mihreteab, Hugo O. Valdivia, Juan F. Sanchez, Khalid B. Beshir, Jane Cunningham, Qin Cheng   +9 more
doaj   +1 more source

The Tumor‐to‐Endothelial Transfer of FTO Promotes Vascular Remodeling and Metastasis in Nasopharyngeal Carcinoma

Advanced Science, EarlyView.
Integrated omics analysis of matched primary and liver metastatic NPC tumors reveals a unique NOTCH1+ CSC subpopulation exhibiting enhanced stemness properties and tumorigenic capacity. With in vitro and in vivo assays, exosomal transfer of tumor‐derived FTO from NOTCH1+ cells to the endothelium promotes vascular permeability and metastatic potential ...
Chun Wu, Xuefei Liu, Liwen Gu, Jingru Lian, Yuting Wang, Yixin Cheng, Lianhui Duan, Guanyin Huang, Siqi Chen, Boxi Zhao, Sailan Liu, Yufan Yang, Shuqian Zheng, Zijian Lu, Wanping Guo, Jianyang Hu, Wenjing Wang, Zhixiang Zuo, Haiqiang Mai, LinQuan Tang, Songfa Zhang, Feiqiu Wen, Xin Hong, Ling Guo   +23 more
wiley   +1 more source

Breakpoint-specific multiplex polymerase chain reaction allows the detection of IKZF1 intragenic deletions and minimal residual disease monitoring in B-cell precursor acute lymphoblastic leukemia

Haematologica, 2013
Deletion of the Ikaros (IKZF1) gene is an oncogenic lesion frequently associated with BCR-ABL1-positive acute lymphoblastic leukemias. It is also found in a fraction of BCR-ABL1-negative B-cell precursor acute lymphoblastic leukemias, and early studies ...
A. Caye, K. Beldjord, K. Mass-Malo, S. Drunat, J. Soulier, V. Gandemer, A. Baruchel, Y. Bertrand, H. Cavé, E. Clappier   +9 more
semanticscholar   +1 more source

PARPi Combining Nanoparticle LIN28B siRNA for the Management of Malignant Ascites

Advanced Science, EarlyView.
This study demonstrates that co‐inhibition of LIN28B and PARP using siLin28b/DSSP@lip‐PEG‐FA nanoparticles in combination with the PARP inhibitor BMN673 effectively suppresses the accumulation of malignant ascites associated with advanced cancers.
Yan Fang, Qian Shen, Yao Lin, Jing Zhu, Xiaolan Zhu, Rui Huang, Yijia Wu, Feiyang Shen, Qian Li, Guopei Zheng, Zhe Zhang, Qian Chu, Junhao Hu, Jianfeng Shen   +13 more
wiley   +1 more source

Elemene Augments the Effects of Anti‐PD‐1 Immunotherapy on Hepatocellular Carcinoma by Regulating the miR‐130a‐5p/SPP/MHC‐I Axis

Advanced Science, EarlyView.
Elemene increases SPP expression by competitively binding with miR‐130a‐5p to suppress SPP mRNA degradation. This led to more antigen/MHC‐I complexes being expressed on the cell surface, which consequently facilitated the recognition and killing of HCC cells by CTLs and enhancing the antitumor immune efficacy of anti‐PD‐1.
Menglan Wang, Mengqing Sun, Heng Dong, Xiaoting Zhang, Jingbo Zhang, Zhengguo Zhang, Mengjie Ni, Lina Li, Yunxin Pei, Xiaoyu Chen, Qian Li, Fangtian Bu, Jiacheng Huang, Liangyu Jiang, Zhuting Fang, Xuliang Chen, Jianxiang Chen, Yiting Qiao, Tian Xie   +18 more
wiley   +1 more source

Molecular typing of Brucella species in human brucellosis cases from Eastern Türkiye

Revista Científica
Brucellosis remains a critical zoonotic infection with profound implications for public health across diverse regions, including the Middle East, Asia, the Arabian Peninsula, the Mediterranean, Africa, and South and Central American countries.
İbrahim Halil Şahin, Sevil Erdenliğ Gürbilek, Nida Özcan, Ahmet Murat Saytekin, Ayfer Güllü Yücetepe   +4 more
doaj   +1 more source

Identification of Salmonella enteritidis isolates by polymerase chain reaction and multiplex polymerase chain reaction.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi, 2003
The polymerase chain reaction and the multiplex polymerase chain reaction were developed for detection of Salmonella and for identification of the serotype enteritidis. Three sets of primers were selected from different genomic sequences amplifying a 429 bp fragment specific for the genus Salmonella within a randomly cloned sequence, including a 250 bp
Tzu-Ming, Pan, Yi-Ju, Liu
openaire   +1 more source