Results 81 to 90 of about 8,966 (157)

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

Single Multiplex Polymerase Chain Reaction To Detect Diverse Loci Associated with Diarrheagenic Escherichia coli

, 2003
We developed and tested a single multiplex polymerase chain reaction (PCR) that detects enterotoxigenic, enteropathogenic, enteroinvasive, and Shiga-toxin–producing Escherichia coli. This PCR is specific, sensitive, and rapid in detecting target isolates
Catalina López-Saucedo, Teresa Estrada-García, Rocío Thompson, F. Raul Velazquez, Phillip I. Tarr, Nicolas Villegas-Sepulveda, Javier Torres, Jorge F. Cerna   +7 more
core   +1 more source

Summary of total reads and read mapping to Hantaan virus (HTNV) reference genome by HTNV multiplex polymerase chain reaction-based next generation sequencing.

, 2021
Summary of total reads and read mapping to Hantaan virus (HTNV) reference genome by HTNV multiplex polymerase chain reaction-based next generation sequencing.
Geum-Young Lee (9504858), Dong Hyun Song (2837216), Hayne Cho Park (8717565), Jongwoo Kim (1308468), Seungchan Cho (589551), Seung-Ho Lee (201595), Kyungmin Park (2263549), Jin Sun No (2837210), Yongjin Yi (8752206), Jin-Won Song (262176), Jingyeong Lee (10789614), Se Hun Gu (9355445), Seong Tae Jeong (2837207), Daesang Lee (795016), Jeong-Ah Kim (81814), Won-Keun Kim (3966437), Jaehun Jung (600064)   +16 more
core   +1 more source

Comparison of prevalence estimates of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum determined by conventional PCR and multiplex qPCR and implications for surveillance and monitoring

International Journal of Infectious Diseases
Objectives: The accuracy of malaria rapid diagnostic tests is threatened by Plasmodium falciparum with pfhrp2/3 deletions. This study compares gene deletion prevalence determined by multiplex real time polymerase chain reaction (qPCR) and conventional ...
Michelle L. Gatton, David Smith, Cielo Pasay, Karen Anderson, Selam Mihreteab, Hugo O. Valdivia, Juan F. Sanchez, Khalid B. Beshir, Jane Cunningham, Qin Cheng   +9 more
doaj   +1 more source

Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots

Nanoscale Research Letters, 2011
Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction (PCR). Here, CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance
Liang Gaofeng, Ma Chao, Zhu Yanliang, Li Shuchun, Shao Youhua, Wang Yong, Xiao Zhongdang   +6 more
doaj  

Detection by multiplex polymerase chain reaction and typing of Chlamydia trachomatis isolates

, 1995
The multiplex polymerase chain reaction (PCR) was applied for the detection of the Chlamydia trachomatis chromosome and plasmid. The multiplex PCR demonstrated a sensitivity of 0.8 fg of chlamydial DNA, corresponding to the detection of about 5 copies of
Buffi C., Valensin P. E., Cusi M. G., Valassina M., Corsaro D., Piazzesi G.   +5 more
core   +1 more source

Molecular typing of Brucella species in human brucellosis cases from Eastern Türkiye

Revista Científica
Brucellosis remains a critical zoonotic infection with profound implications for public health across diverse regions, including the Middle East, Asia, the Arabian Peninsula, the Mediterranean, Africa, and South and Central American countries.
İbrahim Halil Şahin, Sevil Erdenliğ Gürbilek, Nida Özcan, Ahmet Murat Saytekin, Ayfer Güllü Yücetepe   +4 more
doaj   +1 more source

Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces

, 2003
A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and
Phillips, N.D., La, T., Hampson, D.J.
core  

Clinical Utility of Molecular Diagnostics in Children with Respiratory Infections

Bezmiâlem Science
Objective: Lower respiratory tract infections (LRTI) are among the major causes of mortality in children worldwide. Our study aimed to determine viral agents in children under five years of age who were followed up with acute LRTI.
İbrahim TÜRK, Bilge SÜMBÜL, Burcu BURSAL DURAMAZ, Ufuk ERENBERK, Feyza USTABAŞ KAHRAMAN, Özden TÜREL   +5 more
doaj   +1 more source

Detecção por PCR de milho geneticamente modificado (MON810) em farinha de milho, fubá, biju e polenta [PDF]

, 2007
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Agrárias. Programa de Pós-graduação em Ciência dos AlimentosNeste trabalho, o método da Reação em Cadeia da Polimerase (PCR) foi utilizado para detecção do milho ...
Dinon, Andréia Zilio
core