Results 171 to 180 of about 129,024 (210)

Reaction-Mapped Quantitative Multiplexed Polymerase Chain Reaction on a Microfluidic Device

Analytical Chemistry, 2008
We have applied multiple-time-point reaction mapping to generate high-dynamic-range quantitative data from PCR multiplexes. The approach measures, then compensates, numerous PCR slope nonidealities across the multiplex without prejudice. A multilane microelectophoresis device with a novel scanning detector that reports redundantly over more than six ...
Jörn, Ueberfeld, Brian, McKenna, Ifat, Rubin-Bejerano, Kevin, Verstrepen, Daniel J, Ehrlich   +4 more
openaire   +2 more sources

Multiplex Quantitative Fluorescent Polymerase Chain Reaction for Detection of Aneuploidies

Genetic Testing and Molecular Biomarkers, 2012
Quantitative fluorescent polymerase chain reaction (QF-PCR) is a well-established method in Western countries where commercial kits are being used for rapid prenatal diagnosis of aneuploidies. The limitations of these kits are that these are basically designed for the Caucasian population and are very expensive.
Shalu, Jain, Inusha, Panigrahi, Rekha, Gupta, Shubha R, Phadke, Sarita, Agarwal   +4 more
openaire   +2 more sources

Multiplex polymerase chain reaction.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 1992
The polymerase chain reaction (PCR) is a widely utilized assay for specifically amplifying small fragments of DNA. Multiplex PCR is the amplification of more than one DNA fragment per reaction and has many potential uses. When more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced ...
L J, Burgart, R A, Robinson, M J, Heller, W W, Wilke, O K, Iakoubova, J C, Cheville   +5 more
openaire   +1 more source

Comparison of DNA extraction methods for multiplex polymerase chain reaction

Analytical Biochemistry, 2010
We compared six DNA extraction methods for obtaining DNA from whole blood and saliva for use in multiplex polymerase chain reaction (PCR) assays. The aim was to evaluate saliva sampling as an alternative to blood sampling to obtain DNA for molecular diagnostics, genetic genealogy, and research purposes.
Triin, Viltrop, Kaarel, Krjutskov, Priit, Palta, Andres, Metspalu   +3 more
openaire   +2 more sources

Multiplex Polymerase Chain Reaction Diagnostics of Bed Bug (Hemiptera: Cimicidae)

Journal of Medical Entomology, 2011
Cimex lectularius L. (Hemiptera: Cimicidae) is a widespread blood feeding pest of humans around the world, including North America, and has recently undergone a resurgence. A molecular diagnostic technique applying multiplex polymerase chain reaction (PCR) was developed to distinguish bed bug eggs, leg fragments, and degraded samples from other ...
Allen L, Szalanski, Amber D, Tripodi, James W, Austin   +2 more
openaire   +2 more sources

Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia*

Critical Care Medicine, 2008
To test a multiplex real-time polymerase chain reaction (PCR) method for simultaneous detection of multiple organisms in bloodstream infections.Prospective observational study at the University of California Davis Medical Center (Sacramento, CA). Two hundred adult (>18 yrs) patients from the emergency room, intensive care units, and general medicine ...
Richard F, Louie, Zuping, Tang, Timothy E, Albertson, Stuart, Cohen, Nam K, Tran, Gerald J, Kost   +5 more
openaire   +2 more sources

A Multiplex Polymerase Chain Reaction–Based Diagnostic Method for Bacterial Vaginosis

Obstetrics & Gynecology, 2002
To develop a polymerase chain reaction (PCR)-based diagnostic method for bacterial vaginosis using bacterial vaginosis-associated anaerobes.A multiple PCR assay was developed using primers specific to 16S ribosomal deoxyribonucleic acid (DNA) (Mobiluncus mulieris and Mobiluncus curtisii), nanH (Bacteroides fragilis), and an internal spacer region of ...
Mana, Obata-Yasuoka, William, Ba-Thein, Hiromi, Hamada, Hideo, Hayashi   +3 more
openaire   +2 more sources

Detection and typing of lymphotropic herpesviruses by multiplex polymerase chain reaction

Journal of Virological Methods, 1999
A multiplex nested-polymerase chain reaction (PCR) method was developed for the simultaneous detection and typing of all human lymphotropic herpesviruses described to date, including Ebstein Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6, variants A and B (HHV6-A, HHV6-B), human herpesvirus 7 (HHV7) and human herpesvirus 8 (HHV8 ...
F, Pozo, A, Tenorio
openaire   +2 more sources

Multiplex polymerase chain reaction for sex determination of single mouse blastomeres

Molecular Human Reproduction, 1995
Using a multiplex nested polymerase chain reaction (PCR) method with single copy genes and a dinucleotide repeat locus for the mouse Y and X chromosomes respectively, it was possible to discriminate between single cells derived from male and female embryos. Using single cells, amplification of Sry and Zfy sequences was not evident in all cases.
J C, Dreesen, J C, Dumoulin, J L, Evers, J P, Geraedts, M H, Pieters   +4 more
openaire   +2 more sources

Assembled Step Emulsification Device for Multiplex Droplet Digital Polymerase Chain Reaction

Analytical Chemistry, 2019
Digital PCR is a powerful method for absolute nucleic acid quantification with unprecedented accuracy and precision. To promote the wider use and application of digital PCR, several major challenges still exist, including reduction of cost, integration of the instrumental platform, and simplification of operations.
Mengyue Nie, Meng Zheng, Caiming Li, Feng Shen, Manhua Liu, Haibei Luo, Xiaohui Song, Ying Lan, Jian-Zhang Pan, Wenbin Du   +9 more
openaire   +2 more sources