Results 11 to 20 of about 25,229 (229)

Multiplex qRT-PCR for the Detection of Western Equine Encephalomyelitis, St. Louis Encephalitis, and West Nile Viral RNA in Mosquito Pools (Diptera: Culicidae). [PDF]

J Med Entomol, 2015
Following the introduction of West Nile virus into California during the summer of 2003, public health and vector control programs expanded surveillance efforts and were in need of diagnostics capable of rapid, sensitive, and specific detection of arbovirus infections of mosquitoes to inform decision support for intervention. Development of a multiplex
Brault AC, Fang Y, Reisen WK.
europepmc   +4 more sources

High throughput microRNA profiling: optimized multiplex qRT-PCR at nanoliter scale on the fluidigm dynamic arrayTM IFCs. [PDF]

J Vis Exp, 2011
The broad involvement of miRNAs in critical processes underlying development, tissue homoeostasis and disease has led to a surging interest among the research and pharmaceutical communities. To study miRNAs, it is essential that the quantification of microRNA levels is accurate and robust.
Moltzahn F, Hunkapiller N, Mir AA, Imbar T, Blelloch R.   +4 more
europepmc   +4 more sources

Validation of a multiplex qRT-PCR assay for the detection of RSV, influenza A/B virus and SARS-CoV-2 [PDF]

BMC Microbiology
The significant burden of viral respiratory diseases necessitates rapid detection of key pathogens. Simultaneous testing for SARS-CoV-2, RSV, and influenza A/B as an initial step, followed by broader panels as needed, offers a cost-effective diagnostic ...
Alihan Bulgurcu, Ayça Arzu Sayıner
doaj   +2 more sources

Development of a competition assay to assess the in vitro fitness of dengue virus serotypes using an optimized serotype-specific qRT-PCR. [PDF]

PLoS ONE
BackgroundComparing the in vitro fitness of dengue virus (DENV) isolates is a pivotal approach to assess the contribution of DENV strains' replicative fitness to epidemiological contexts, including serotype replacements.
Anne-Fleur Griffon, Loeïza Rault, Clément Tanvet, Etienne Simon-Lorière, Myrielle Dupont-Rouzeyrol, Catherine Inizan   +5 more
doaj   +2 more sources

Development of a multiplex real-time qRT-PCR for discriminating the predominant epidemic variant IBDV and very virulent IBDV [PDF]

Frontiers in Veterinary Science
Infectious bursal disease (IBD) is an important immunosuppressive disease of chicken caused by infectious bursal disease virus (IBDV). At present, the newly emerging novel variant IBDV (varIBDV) and the persistently prevalent very virulent IBDV (vvIBDV ...
Ziwen Wu, Ziwen Wu, Hangbo Yu, Hangbo Yu, Guodong Wang, Guodong Wang, Dan Ling, Dan Ling, Yulong Zhang, Yulong Zhang, Runhang Liu, Runhang Liu, Erjing Ke, Suyan Wang, Suyan Wang, Yanping Zhang, Yongzhen Liu, Hongyu Cui, Yuntong Chen, Yulu Duan, Xianyun Liu, Yulong Gao, Yulong Gao, Yulong Gao, Yulong Gao, Xiaole Qi, Xiaole Qi, Xiaole Qi, Xiaole Qi   +28 more
doaj   +2 more sources

Microfluidic-based multiplex qRT-PCR identifies diagnostic and prognostic microRNA signatures in the sera of prostate cancer patients. [PDF]

Cancer Res, 2011
Abstract Recent prostate-specific antigen–based screening trials indicate an urgent need for novel and noninvasive biomarker identification strategies to improve the prediction of prostate cancer behavior. Noncoding microRNAs (miRNA) in the serum and plasma have been shown to have potential as noninvasive markers for physiologic and ...
Moltzahn F, Olshen AB, Baehner L, Peek A, Fong L, Stöppler H, Simko J, Hilton JF, Carroll P, Blelloch R.   +9 more
europepmc   +6 more sources

hsa_circ_0006168 drives microglial activation in TNC via miR-99b-5p/KDM6B axis to promote central sensitization in migraine [PDF]

The Journal of Headache and Pain
Background Migraine is a disabling neurological disorder characterized by recurrent headache attacks and associated symptoms. The mechanisms underlying migraine remain unclear. This study aimed to identify differentially expressed circular RNAs (circRNAs)
Qihui Chen, Qingling Zhai, Jinghan Lin, Yanan Huang, Hongyan Li, Changman Zhang, Qijun Yu, Tingting Sun, Yonghui Pan   +8 more
doaj   +2 more sources

Nanocurcumin: An Innovative Strategy to Combat Virulence and Methicillin Resistance in Staphylococcus aureus Isolated from Burn Wounds [PDF]

Iranian Journal of Medical Sciences
Background: Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen that causes various infections and exhibits antibiotic resistance and virulence factors, requiring alternative therapies.
Alaa Alhameedawi, Majid Sadeghizadeh, Bahaa Abdullah Al-Rubaii   +2 more
doaj   +2 more sources

Advancing IBDV diagnostics: a one-step multiplex real-time qRT-PCR for discriminating between vvIBDV and non-vvIBDV viruses, including the newly emerged IBDV variant

Frontiers in Veterinary Science
The very virulent infectious bursal disease virus (vvIBDV) induces an acute, highly contagious and immunosuppressive disease in younger chicken causing massive economic losses globally.
Amany Adel, Ali Zanaty, Zienab Mosaad, Karim Selim, Naglaa M. Hagag, Mona Badr, Hany Ellakany, Momtaz Shahien, Ahmed Samy, Ahmed Samy   +9 more
doaj   +3 more sources

Multiplex Profiling of Cellular Invasion in 3D Cell Culture Models. [PDF]

, 2012
To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces.
Burgstaller, Gerald, Eickelberg, Oliver, Koch, Ina, Lindner, Michael, Oehrle, Bettina   +4 more
core   +14 more sources