Results 21 to 30 of about 25,229 (229)

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus

Animals, 2023
Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Feng Long, Yating Chen, Kaichuang Shi, Yanwen Yin, Shuping Feng, Hongbin Si   +5 more
doaj   +1 more source

Differentiating cutaneous squamous cell carcinoma and pseudoepitheliomatous hyperplasia by multiplex qRT-PCR [PDF]

Modern Pathology, 2013
Squamous cell carcinoma is the second most common cutaneous malignancy. The diagnosis can occasionally be difficult as there are many lesions that are mimics, clinically and on pathologic examination. One of the most challenging lesions to differentiate from squamous cell carcinoma is pseudoepitheliomatous hyperplasia, a reactive proliferation of the ...
Albert, Su, Seong, Ra, Xinmin, Li, Jian, Zhou, Scott, Binder   +4 more
openaire   +2 more sources

One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples [PDF]

PeerJ, 2016
Background. Group A rotavirus (RVA) infection is the major cause of acute gastroenteritis (AGE) in young children worldwide. Introduction of two live-attenuated rotavirus vaccines, RotaTeq® and Rotarix®, has dramatically reduced RVA associated AGE and ...
Rashi Gautam, Slavica Mijatovic-Rustempasic, Mathew D. Esona, Ka Ian Tam, Osbourne Quaye, Michael D. Bowen   +5 more
doaj   +2 more sources

A Multiplex Crystal Digital PCR for Detection of African Swine Fever Virus, Classical Swine Fever Virus, and Porcine Reproductive and Respiratory Syndrome Virus

Frontiers in Veterinary Science, 2022
African swine fever (ASF), classical swine fever (CSF), and porcine reproductive and respiratory syndrome (PRRS) are highly infectious diseases of domestic pigs and wild boars.
Kaichuang Shi, Kaichuang Shi, Yating Chen, Yanwen Yin, Feng Long, Shuping Feng, Huixin Liu, Sujie Qu, Hongbin Si   +8 more
doaj   +1 more source

Multiplexing primer/probe sets for detection of SARS-CoV-2 by qRT-PCR

Journal of Clinical Virology, 2020
The novel respiratory virus SARS-CoV-2, responsible for over 380,000 COVID-19 related deaths, has caused significant strain on healthcare infrastructure and clinical laboratories globally. The pandemic's initial challenges include broad diagnostic testing, consistent reagent supply lines, and access to laboratory instruments and equipment.
Perchetti, Garrett A., Nalla, Arun K., Huang, Meei-Li, Jerome, Keith R., Greninger, Alexander L.   +4 more
openaire   +2 more sources

A Quadruplex qRT-PCR for Differential Detection of Four Porcine Enteric Coronaviruses

Veterinary Sciences, 2022
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) are four identified porcine enteric coronaviruses.
Hongjin Zhou, Kaichuang Shi, Feng Long, Kang Zhao, Shuping Feng, Yanwen Yin, Chenyong Xiong, Sujie Qu, Wenjun Lu, Zongqiang Li   +9 more
doaj   +1 more source

Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology [PDF]

Genomics & Informatics, 2013
RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and ...
Jong-Lyul Park, Seong-Min Park, Jeong-Hwan Kim, Han-Chul Lee, Seung-Hwan Lee, Kwang-Man Woo, Seon-Young Kim   +6 more
doaj   +1 more source

Evaluation of Asymptomatic Malaria Status in Eastern of Afghanistan Using High Resolution Melting Analysis

Iranian Journal of Parasitology, 2020
Background: Malaria is threatening more than half of Afghanistan population. Asymptomatic malaria is notable problem against malaria controlling strategies.
Sayed Hussain MOSAWI, Abdolhossein DALIMI, Najibullah SAFI, Fatemeh GHAFFARIFAR, Javid SADRAEI   +4 more
doaj   +1 more source

An Advanced Multiplex Real-Time Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid and Reliable Detection of Porcine Epidemic Diarrhea Virus and Porcine Internal Positive Control

Viruses, 2023
For rapid and reliable detection of porcine epidemic diarrhea virus (PEDV) from pig clinical samples, a multiplex, real-time, reverse transcription loop-mediated isothermal amplification (mqRT-LAMP) was developed using two sets of primers and ...
Hye-Ryung Kim, Jong-Min Kim, Ji-Su Baek, Jonghyun Park, Won-Il Kim, Bok Kyung Ku, Hye-Young Jeoung, Kyoung-Ki Lee, Choi-Kyu Park   +8 more
doaj   +1 more source

Comparative Transcript Profiling and Multiplex qRT-PCR Analysis Between Salt-Tolerant and Sensitive Wheat Genotypes

Global Journal Of Botanical Science, 2023
Identification of candidate genes combined with gene expression profiling carries importance to facilitate the molecular basis of salt stress response in plants. Here, cDNA-AFLP was used to compare the transcribed sequences among two bread and two durum wheat genotypes with different levels of salt tolerance.
openaire   +1 more source