Results 11 to 20 of about 283 (168)

Human Mitochondrial Control Region and mtGenome: Design and Forensic Validation of NGS Multiplexes, Sequencing and Analytical Software

, 2021
Forensic mitochondrial DNA (mtDNA) analysis conducted using next-generation sequencing (NGS), also known as massively parallel sequencing (MPS), as compared to Sanger-type sequencing brings modern advantages, such as deep coverage per base (herein ...
Kathryn M. Stephens, Elmira Forouzmand, Shan-Fu Wu, Keenan D. Fleming, Cydne L. Holt, Paulina Walichiewicz   +5 more
core   +1 more source

Selection strategies for marker-assisted background selection with chromosome-wise SSR multiplexes in pseudo-backcross programs for grapevine breeding

, 2015
Organizing SSR markers located on one chromosome into PCR multiplexes has the potential to reduce the costs of marker analysis. The optimal selection strategies for such chromosome-wise multiplexes have not yet been investigated.
Töpfer, Reinhard, Frisch, M., Herzog, E., Hausmann, Ludger, Eibach, Rudolf   +4 more
core   +2 more sources

Characterization of two microsatellite PCR multiplexes for high throughput genotyping of the Caribbean spiny lobster, Panulirus argus

, 2015
The spiny lobster Panulirus argus supports one of the most economically important commercial fisheries in the Caribbean, yet its sustainable management is problematic due to uncertainty regarding levels of population connectivity among Caribbean nations.
Nathan Truelove, Mark Butler IV, Richard F Preziosi, Donald Behringer Jr.   +3 more
core   +2 more sources

Development of a Multiplex PCR Short Tandem Repeat Typing Scheme for Candida krusei

, 2022
Candida krusei is a human-pathogenic yeast that can cause candidemia with the lowest 90-day survival rate in comparison to other Candida species. Infections occur frequently in immunocompromised patients, and several C.
de Groot, Theun, Spruijtenburg, Bram, Jain, Kusum, Hanson, Kimberly E., Haren, Merlijn H. van, van Haren, Merlijn H. I., Meis, Jacques F., Meis, J.F., Chowdhary, Anuradha, Groot, Theun de   +9 more
core   +1 more source

Electrophoretogram of PCR products from multiplexes N-I to N-VI.

, 2016
The primer sets of multiplexes N-I to N-VI are shown in Table 2.Yellow, light blue, light green, red, green, and purple frames indicate multiplexes N-I, II, III, IV, V, and VI, respectively. The color coding corresponds to that given in Fig 2.
Phrabhakaran Nambiar (697531), Hideki Shojo (476368), Tsuneo Kakuda (797452), Kiyoshi Minaguchi (3161268), Noboru Adachi (424979), Mayumi Tanaka (255424), Kazuo Umetsu (3161265)   +6 more
core   +1 more source

Electrophoretogram of PCR products from multiplexes M-I to M-VIII.

, 2016
The primer sets of multiplexes M-I to M-VIII are shown in Table 2. Yellow, light blue, light green, red, green, purple, orange, and blue frames indicate multiplexes M-I, II, III, IV, V, VI, VII, and VIII, respectively.
Phrabhakaran Nambiar (697531), Hideki Shojo (476368), Tsuneo Kakuda (797452), Kiyoshi Minaguchi (3161268), Noboru Adachi (424979), Mayumi Tanaka (255424), Kazuo Umetsu (3161265)   +6 more
core   +1 more source

ESR1 methylation and ESR1 mutations in circulating tumor cells (CTCs) and paired plasma‐cfDNA of advanced breast cancer patients: A feasibility proof‐of‐concept study

Molecular Oncology, EarlyView.
Circulating tumor cells (CTCs) and plasma cell‐free DNA (cfDNA) were analyzed to detect ESR1 mutations and methylation in patients with advanced breast cancer. CTC‐derived DNA showed higher sensitivity for mutation detection and revealed complementary genetic and epigenetic alterations, highlighting the added value of CTC analysis for understanding ...
Dimitra Stergiopoulou, Athina Markou, Eleonora Nicolo, Mara S Serafini, Qiang Zhang, Youbin Zhang, Lorenzo Gerratana, Andrew A. Davis, Huiping Liu, William J Gradishar, Carolina Reduzzi, Massimo Cristofanilli, Evi Lianidou   +12 more
wiley   +1 more source

Developmental programmes drive cellular plasticity, disease progression and therapy resistance in lung adenocarcinoma

Molecular Oncology, EarlyView.
This study shows that lung adenocarcinomas exploit developmental branching morphogenesis to acquire a therapy resistant basal‐like tumour cell state. This process was found to be regulated by combined TP53 loss‐of‐function and type‐I interferon signalling, identifying a novel axis for biomarker and therapeutic target discovery.
Kamila J Bienkowska, Stephany Gallardo, Nur S. Zainal, Leena Arora, Matthew Ellis, Maria‐Antoinette Lopez, Judith Austine, Sai Pittla, Serena J Chee, Aiman Alzetani, Emily C Shaw, Christian H Ottensmeier, Gareth J Thomas, Christopher J Hanley   +13 more
wiley   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Emerging insights into CC and CXC chemokines and their receptors in Mycobacterium tuberculosis infection

FEBS Open Bio, EarlyView.
The dual roles of CC and CXC chemokines in distinguishing active, latent, and subclinical tuberculosis were reviewed, along with an evaluation of their potential as diagnostic biomarkers and therapeutic targets to advance precision medicine in tuberculosis management. The graphical abstract was generated with AI assistance (Gemini 3.0).
Xuying Yin, Dangsheng Xiao, Jiezuan Yang
wiley   +1 more source