Results 1 to 10 of about 1,536 (226)
SIMPLseq: a high-sensitivity Plasmodium falciparum genotyping and PCR contamination tracking tool [PDF]
Malaria JournalBackground Pathogen genotyping via polymerase chain reaction (PCR) amplicon sequencing (AmpSeq) is an informative disease surveillance tool. Several large AmpSeq panels containing > 100 multiplexed PCR amplicons have been developed as alternatives to ...
Philipp Schwabl +6 more
doaj +2 more sources
The Iraqi Journal of Medical Sciences, 2021 Background: The blood of blood donors is screening for hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) infection by enzyme immunoassay (EIA) in the National Center of Blood Bank in Baghdad.
Ghinwa S. Majid +4 more
doaj +9 more sources
Frontiers in Cellular and Infection Microbiology, 2022 Cryptococcus neoformans (C. neoformans)/C. gattii can easily invade the human central nervous system and cause cryptococcal meningitis (CM). The clinical fatality rate of these fungi is extremely high and causes more than 180,000 deaths worldwide every ...
Lei Wang +17 more
doaj +1 more source
Scientific Reports, 2023 A panel of X-linked microsatellite markers was newly designed using the data from a previous sequencing project available in NCBI and used for a study of the Colorado potato beetle (CPB, Leptinotarsa decemlineata) X-haplotype variability. The analysis of
P. Sedlák +8 more
doaj +1 more source
Genotyping by multiplexed sequencing (GMS): A customizable platform for genomic selection.
PLoS ONE, 2020 As genotyping technologies continue to evolve, so have their throughput and multiplexing capabilities. In this study, we demonstrate a new PCR-based genotyping technology that multiplexes thousands of single nucleotide polymorphism (SNP) markers with ...
Travis M Ruff +6 more
doaj +1 more source
SINGLE NUCLEOTIDE POLYMORPHISMS APPROACH FOR GENETIC BACKGROUND OF ICR MICE USING MASSARRAY [PDF]
Exploratory Animal and Medical ResearchSingle nucleotide polymorphisms (SNPs) are an effective method for determining the genetic background of ICR outbred mice in the National Laboratory Animal Center, Mahidol University. We screened 20 SNPs on all autosomes and X chromosome.
Nopparat Phumkumarn +3 more
doaj +1 more source
Identification of a criminal by DNA typing in a rape case in Rio de Janeiro, Brazil
São Paulo Medical Journal, 2002 CONTEXT: Human DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains.
Andréa Carla de Souza Góes +4 more
doaj +1 more source
BioTechniques, 2002 The analysis of short tandem repeat (STR) DNA sequences is of fundamental importance to forensic science because they have become the recognized standard in constructing national public databases.
Peter Gill
doaj +1 more source
Fundamentals of multiplexing with digital PCR
Biomolecular Detection and Quantification, 2016 Over the past decade numerous publications have demonstrated how digital PCR (dPCR) enables precise and sensitive quantification of nucleic acids in a wide range of applications in both healthcare and environmental analysis. This has occurred in parallel with the advances in partitioning fluidics that enable a reaction to be subdivided into an ...
Whale, Alexandra S. +2 more
openaire +2 more sources
Multiplex PCR for identifying mycobacterial isolates. [PDF]
Journal of Clinical Pathology, 1995 AIMS--To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. METHODS--Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling in TE Triton. The lysate (5 microliters) was used directly in a PCR reaction incorporating three pairs of PCR primers expected to ...
Cormican, M +3 more
openaire +3 more sources