Results 21 to 30 of about 807,352 (165)
Multiplex PCR for identifying mycobacterial isolates. [PDF]
Journal of Clinical Pathology, 1995 AIMS--To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. METHODS--Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling in TE Triton. The lysate (5 microliters) was used directly in a PCR reaction incorporating three pairs of PCR primers expected to ...
Cormican, M +3 more
openaire +3 more sources
Development and transferability of two multiplexes nSSR in Scots pine (Pinus sylvestris L.)
, 2015 Single sequence repeat (SSR) multiplexing is a semi high-throughput PCR methodology for the analysis of multiple SSRs. We developed two SSR multiplexes selected from SSR loci previously reported in the pine literature and tested the transferability of ...
Ganea, Laura Stefana, +7 more
core +1 more source
MegaPlex PCR: a strategy for multiplex amplification [PDF]
Nature Methods, 2007 'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair.
Meuzelaar, Linda Strömqvist +4 more
openaire +2 more sources
Electrophoretogram of PCR products from multiplexes N-I to N-VI.
, 2016 The primer sets of multiplexes N-I to N-VI are shown in Table 2.Yellow, light blue, light green, red, green, and purple frames indicate multiplexes N-I, II, III, IV, V, and VI, respectively. The color coding corresponds to that given in Fig 2.
Phrabhakaran Nambiar (697531) +6 more
core +1 more source
Betweenness centrality for temporal multiplexes [PDF]
, 2021 Betweenness centrality quantifies the importance of a vertex for the information flow in a network. The standard betweenness centrality applies to static single-layer networks, but many real world networks are both dynamic and made of several layers.
Mazzarisi, Piero +8 more
core +1 more source
Electrophoretogram of PCR products from multiplexes M-I to M-VIII.
, 2016 The primer sets of multiplexes M-I to M-VIII are shown in Table 2. Yellow, light blue, light green, red, green, purple, orange, and blue frames indicate multiplexes M-I, II, III, IV, V, VI, VII, and VIII, respectively.
Phrabhakaran Nambiar (697531) +6 more
core +1 more source
, 2011 International audienceMultiplex PCR is a fast and cost-effective technique allowing increased genotyping throughput of microsatellites. We developed two multiplexes for Quercus petraea and Q.
R.J. PETIT +15 more
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Real-Time PCR and Multiplex Approaches [PDF]
, 2011 Analysis of RNA expression levels by real-time reverse-transcription (RT) PCR has become a routine technique in diagnostic and research laboratories. Monitoring of DNA amplification can be done using fluorescent sequence-specific probes, which generate signal only upon binding to their target.
Olga L, Gurvich, Mikhail, Skoblov
openaire +2 more sources
Primer1: Primer design Web service for Tetra-Primer ARMS-PCR [PDF]
, 2012 Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of ...
Ke, Xiayi, Collins, Andrew
core +1 more source