Compromised MPS1 Activity Induces Multipolar Spindle Formation in Oocytes From Aged Mares: Establishing the Horse as a Natural Animal Model to Study Age-Induced Oocyte Meiotic Spindle Instability [PDF]
Aneuploidy originating during meiosis in oocytes is the major cause of reduced fertility, implantation failure and miscarriage in women beyond their mid-thirties. Loss of chromosome cohesion, and defective microtubule dynamics and spindle assembly are, in turn, the major contributors to the error-prone nature of chromosome segregation in the oocytes of
Marilena Rizzo+5 more
openalex +8 more sources
A novel microtubule-modulating noscapinoid triggers apoptosis by inducing spindle multipolarity via centrosome amplification and declustering [PDF]
We have previously shown that a non-toxic noscapinoid, EM011 binds tubulin without altering its monomer/polymer ratio. EM011 is more active than the parent molecule, noscapine, in inducing G2/M arrest, inhibiting cellular proliferation and tumor growth in various human xenograft models. However, the mechanisms of mitotic-block and subsequent cell death
PRIYAMBADA KARNA+8 more
openalex +3 more sources
Toxicological studies of arsenic compounds were conducted in cultured mammalian cells to investigate the effects of glutathione (GSH) depletion. Dimethylarsinate DMA(V) was not cytotoxic in cells depleted of GSH, but was found to be cytotoxic when GSH was present outside the cells.
Takafumi Ochi
openalex +4 more sources
The oncogene cyclin D1 promotes bipolar spindle integrity under compressive force. [PDF]
The mitotic spindle is the bipolar, microtubule-based structure that segregates chromosomes at each cell division. Aberrant spindles are frequently observed in cancer cells, but how oncogenic transformation affects spindle mechanics and function ...
Renaldo Sutanto+6 more
doaj +2 more sources
Induction of Multipolar Spindles in the Meiosis of <i>Triticum aestivum</i> as Effected by Acetone
In the present investigation, the treatment of pollen mother cells of Triticum aestivum with acetone provided an excellent material for multipolarity. It was possible to follow the formation of the spindle fibre apparatus in the case of multipolar anaphases.
A. Kabarity
openalex +4 more sources
Normal cell division is coordinated by a bipolar mitotic spindle, ensuring symmetrical segregation of chromosomes. Cancer cells, however, occasionally divide into three or more directions. Such multipolar mitoses have been proposed to generate genetic diversity and thereby contribute to clonal evolution.
David Gisselsson+8 more
openalex +8 more sources
Aurora-A inactivation causes mitotic spindle pole fragmentation by unbalancing microtubule-generated forces [PDF]
Background Aurora-A is an oncogenic kinase playing well-documented roles in mitotic spindle organisation. We previously found that Aurora-A inactivation yields the formation of spindles with fragmented poles that can drive chromosome mis-segregation ...
Asteriti Italia A+3 more
doaj +4 more sources
siRNA-Mediated BmAurora B Depletion Impedes the Formation of Holocentric Square Spindles in Silkworm Metaphase BmN4 Cells [PDF]
Silkworm ovary-derived BmN4 cells rely on chromatin-induced spindle assembly to form microtubule-based square mitotic spindles that ensure accurate segregation of holocentric chromosomes during cell division.
Bing Zhang+5 more
doaj +2 more sources