Results 11 to 20 of about 8,829 (221)

Revisiting the Endocytosis of the M2 Muscarinic Acetylcholine Receptor [PDF]

Membranes, 2015
The agonist-induced endocytosis of the muscarinic acetylcholine receptor M2 is different from that of the other members of the muscarinic receptor family.
Wymke Ockenga, Ritva Tikkanen
doaj   +4 more sources

Non-Neuronal Functions of the M2 Muscarinic Acetylcholine Receptor [PDF]

Genes, 2013
Acetylcholine is an important neurotransmitter whose effects are mediated by two classes of receptors. The nicotinic acetylcholine receptors are ion channels, whereas the muscarinic receptors belong to the large family of G protein coupled seven transmembrane helix receptors.
Ockenga, Wymke, Kühne, Sina, Bocksberger, Simone, Banning, Antje, Tikkanen, Ritva, Justus Liebig University Giessen   +5 more
openaire   +6 more sources

Modulation of the M2 Muscarinic Acetylcholine Receptor Activity with Monoclonal Anti-M2 Receptor Antibody Fragments [PDF]

Journal of Biological Chemistry, 2004
Antibodies directed against the second extracellular loop of G protein-coupled receptors are known to have functional activities. From a partial agonist monoclonal antibody directed against the M2 muscarinic receptor, we constructed and produced a single chain variable fragment with high affinity for its target epitope.
Jean-Christophe, Peter, Gerd, Wallukat, Jean, Tugler, Damien, Maurice, Jean-Christophe, Roegel, Jean-Paul, Briand, Johan, Hoebeke   +6 more
openaire   +3 more sources

Synergistic regulation mechanism of iperoxo and LY2119620 for muscarinic acetylcholine M2 receptor [PDF]

RSC Advances, 2018
Dynamics correlation network was used to reveal the synergistic regulation mechanism of iperoxo and LY2119620 for muscarinic acetylcholine M2 receptor.
Quan Li, Hai-Feng Chen
core   +5 more sources

Expression of Functional M2 Muscarinic Acetylcholine Receptor in Escherichia coli [PDF]

Journal of Biochemistry, 2000
The M2 muscarinic acetylcholine receptor mutant (M2 mutant), with a lack of glycosylation sites, a deletion in the central part of the third inner loop, and the addition of a six histidine tag at the C-terminus, was fused to maltose binding protein (MBP) at its N-terminus and expressed in Escherichia coli. The expression level was 0.2 nmol receptor per
H, Furukawa, T, Haga
openaire   +3 more sources

Structure of the human M2 muscarinic acetylcholine receptor bound to an antagonist [PDF]

Nature, 2012
The parasympathetic branch of the autonomic nervous system regulates the activity of multiple organ systems. Muscarinic receptors are G-protein-coupled receptors that mediate the response to acetylcholine released from parasympathetic nerves. Their role in the unconscious regulation of organ and central nervous system function makes them potential ...
Haga, Kazuko, Kruse, Andrew C., Asada, Hidetsugu, Yurugi-Kobayashi, Takami, Shiroishi, Mitsunori, Zhang, Cheng, Weis, William I., Okada, Tetsuji, Kobilka, Brian K., Haga, Tatsuya, Kobayashi, Takuya   +10 more
openaire   +4 more sources

Role of Conserved Tyrosine Lid Residues in the Activation of the M2 Muscarinic Acetylcholine Receptor

Molecular Pharmacology, 2023
The development of subtype selective small molecule drugs for the muscarinic acetylcholine receptor (mAChR) family has been challenging. The design of more selective ligands can be improved by understanding the structure and function of key amino acid residues that line ligand binding sites.
Vi Pham, Maria Clazina Cornelia Habben Jansen, Geoff Thompson, Laura H. Heitman, Arthur Christopoulos, David M. Thal, Celine Valant   +6 more
openaire   +5 more sources

Lack of m2 muscarinic acetylcholine receptor mRNA in rat lymphocytes

Journal of Neuroimmunology, 1994
The presence of muscarinic acetylcholine receptors on lymphocytes has been demonstrated by radioligand binding experiments. Although the specific subtype(s) of muscarinic acetylcholine receptors expressed in lymphocytes is still unknown, some reports suggest the presence of the m2 subtype.
Costa, P, Castoldi, A. F, Traver, D. J, Costa, L. G.   +3 more
openaire   +3 more sources

Acetylcholine muscarinic M2 receptor maintains human Schwann-like adipose-derived phenotype in the absence of differentiating medium

Cell Death Discovery
Human adipose-derived stem cells (ASCs), differentiated in vitro towards Schwann-like phenotype (hdASCs), have been suggested as an alternative source of Schwann cells (SCs).
Roberta Piovesana, Alessandro Faroni, Ada Maria Tata, Adam J. Reid   +3 more
doaj   +2 more sources

Dualsteric modulators of the M2 muscarinic acetylcholine receptor [PDF]

Journal of Cheminformatics, 2014
G-protein coupled receptors (GPCRs) trigger multiple signal-switching mechanisms like binding of s-arrestin proteins, activation of kinases and G-protein activation [1]. The poor understanding of the conformational changes resulting in these activations is a major challenge for the design of specific GPCR modulating drugs.
Marcel Bermudez, Gerhard Wolber
openaire   +1 more source