Results 11 to 20 of about 28,635 (297)
Long-distance effects of insertional mutagenesis. [PDF]
PLoS ONE, 2011 Most common systems of genetic engineering of mammalian cells are associated with insertional mutagenesis of the modified cells. Insertional mutagenesis is also a popular approach to generate random alterations for gene discovery projects.
Ruchi Singhal +3 more
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Insertional mutagenesis strategies in zebrafish [PDF]
Genome Biology, 2007 We review here some recent developments in the field of insertional mutagenesis in zebrafish. We highlight the advantages and limitations of the rich body of retroviral methodologies, and we focus on the mechanisms and concepts of new transposon-based mutagenesis approaches under development, including prospects for conditional 'gene trapping' and ...
Sivasubbu, Sridhar +3 more
core +4 more sources
Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics
Plants, 2019 Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated ...
Hasthi Ram +6 more
doaj +2 more sources
In vitro HIV DNA integration in STAT3 drives T cell persistence-A model of HIV-associated T cell lymphoma. [PDF]
PLoS PathogensOncogenic retroviruses are known for their pathogenesis via insertional mutagenesis, in which the presence of a provirus and its transcriptional control elements alter the expression of a nearby or surrounding host gene.
Michael Rist +2 more
doaj +2 more sources
Site Preferences of Insertional Mutagenesis Agents in Arabidopsis [PDF]
Plant Physiology, 2005 Abstract We have performed a comparative analysis of the insertion sites of engineered Arabidopsis (Arabidopsis thaliana) insertional mutagenesis vectors that are based on the maize (Zea mays) transposable elements and Agrobacterium T-DNA.
Pan, X., Li, Yong, Stein, L.
openaire +5 more sources
Forward and Reverse Genetics of B Cell Malignancies: From Insertional Mutagenesis to CRISPR-Cas
Frontiers in Immunology, 2021 Cancer genome sequencing has identified dozens of mutations with a putative role in lymphomagenesis and leukemogenesis. Validation of driver mutations responsible for B cell neoplasms is complicated by the volume of mutations worthy of investigation and ...
Joanna C. Dawes +3 more
doaj +3 more sources
Insertional Mutagenesis of Chlamydomonas reinhardtii
Bio-Protocol, 2015 The unicellular microalga Chlamydomonas reinhardtii (C. reinhardtii) has been used as a reference model for numerous fields of research. Principle research areas are eukaryotic flagellar structure and function, basal bodies (centrioles), cell-cell ...
Chia-Hong Tsai, Christoph Benning
doaj +2 more sources
CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit
Nature CommunicationsSite-directed insertion is a powerful approach for generating mutant alleles, but low efficiency and the need for customisation for each target has limited its application.
Lee B. Miles +7 more
doaj +2 more sources
, 2022 Supplementary Data from In Vitro Insertional Mutagenesis Screen Identifies Novel Genes Driving Breast Cancer ...
Csaba Miskey (466684) +8 more
core +7 more sources
Insertional mutagenesis in zebrafish [PDF]
Developmental Dynamics, 2003 AbstractInsertional mutagenesis is a method for identifying genes essential for a given biological process by using the integration of DNA as the mutagen, thereby facilitating the cloning of the mutated gene. The use of retrovirus‐mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes ...
Adam Amsterdam, Nancy Hopkins
openaire +3 more sources