Deregulated Nras expression in knock-in animals harboring a gammaretroviral long terminal repeat at the Nras/Csde1 locus. [PDF]
PLoS ONE, 2013 To investigate mechanisms and phenotypic effects of insertional mutagenesis by gammaretroviruses, we have developed mouse lines containing a single Akv 1-99 long terminal repeat (LTR) and a floxed PGK/Tn5 neomycin cassette at the Nras proto-oncogene at ...
Borja Ballarín-González +5 more
doaj +1 more source
Transposon Insertional Mutagenesis in Rice, [PDF]
Plant Physiology, 2001 Transposon mutagenesis systems are being developed in rice to address gene functions using forward and reverse genetics strategies. Although the well-characterized maize ( Zea mays ) transposons seem an obvious choice to develop efficient transposon-tagging systems for gene knockouts and ...
Greco, R. +9 more
openaire +5 more sources
In vivo insertion pool sequencing identifies virulence factors in a complex fungal-host interaction. [PDF]
PLoS Biology, 2018 Large-scale insertional mutagenesis screens can be powerful genome-wide tools if they are streamlined with efficient downstream analysis, which is a serious bottleneck in complex biological systems.
Simon Uhse +5 more
doaj +1 more source
Mutagenesis by retroviral insertion in chemical mutagen-generated quasi-haploid mammalian cells
BioTechniques, 2007 Diploidy is a major obstacle to the mutagenic analysis of function in cultured mammalian cells. Here, we show that 6–8 rounds of chemical mutagenesis generates quasi-haploid cells that can be used as targets for insertional mutagenesis using a specially ...
Sung O. Kim +5 more
doaj +1 more source
Efficient Transposition of the Retrotransposon Tnt1 in Cucumber (Cucumis sativus L.)
Horticultural Plant Journal, 2018 Tnt1 is an active retrotransposon originally identified in tobacco (Nicotiana tabacum L.) (Grandbastien et al., 1989), but its transposition activity could be activated through tissue culture in other plant species.
Qi ZHANG +9 more
doaj +1 more source
Broad Purpose Vector for Site-Directed Insertional Mutagenesis in Bifidobacterium breve
Frontiers in Microbiology, 2021 Members of the genus Bifidobacterium are notoriously recalcitrant to genetic manipulation due to their extensive and variable repertoire of Restriction-Modification (R-M) systems.
Emily C. Hoedt +14 more
doaj +1 more source
Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
BMC Microbiology, 2018 Background Transposon mutagenesis is highly valuable for bacterial genetic and genomic studies. The transposons are usually delivered into host cells through conjugation or electroporation of a suicide plasmid.
Santa S. Naorem +8 more
doaj +1 more source
Development of mPing‐based activation tags for crop insertional mutagenesis
Plant Direct, 2021 Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits.
Alexander Johnson +12 more
doaj +1 more source
BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage
Nature Communications, 2018 Loss of BRCA2 leads to cancer formation. Here, the authors use an insertional mutagenesis approach and identify a multiprotein complex consisting of BRE, USP7 and CDC25A that can support the survival of BRCA2-deficient cells.
Kajal Biswas +9 more
doaj +1 more source
Magnaporthe grisea Pathogenicity Genes Obtained Through Insertional Mutagenesis
Molecular Plant-Microbe Interactions, 1998 We have initiated a mutational analysis of pathogenicity in the rice blast fungus, Magnaporthe grisea, in which hygromycin-resistant transformants, most generated by restriction enzyme-mediated integration (REMI), were screened for the ability to infect ...
James A. Sweigard +4 more
doaj +1 more source