Results 31 to 40 of about 28,635 (297)

Generation of an Enhancer-Trapping Vector for Insertional Mutagenesis in Zebrafish. [PDF]

PLoS ONE, 2015
Enhancer trapping (ET) is a powerful approach to establish tissue- or cell-specific reporters and identify expression patterns of uncharacterized genes. Although a number of enhancer-trapping vectors have been developed and a large library of fish lines ...
Chunyan Liu, Guili Song, Lin Mao, Yong Long, Qing Li, Zongbin Cui   +5 more
doaj   +1 more source

A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

Plant Methods, 2017
Background Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many instances these insertional mutants
Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman, James V. Moroney   +6 more
doaj   +1 more source

Safe engineering of CAR T cells for adoptive cell therapy of cancer using long‐term episomal gene transfer

EMBO Molecular Medicine, 2016
Chimeric antigen receptor (CAR) T‐cell therapy is a new successful treatment for refractory B‐cell leukemia. Successful therapeutic outcome depends on long‐term expression of CAR transgene in T cells, which is achieved by delivering transgene using ...
Chuan Jin, Grammatiki Fotaki, Mohanraj Ramachandran, Berith Nilsson, Magnus Essand, Di Yu   +5 more
doaj   +1 more source

Insertion of the LINE Retrotransposon MGL Causes a Conidiophore Pattern Mutation in Magnaporthe grisea

Molecular Plant-Microbe Interactions, 2000
We obtained three Magnaporthe grisea morphological mutants that had the LINE transposon MGL inserted into the ACR1 locus. Sequence analysis revealed that ACR1 is homologous to medA, a developmental regulator of Aspergillus nidulans conidiation.
Marie Nishimura, Nagao Hayashi, Nam-Soo Jwa, Gee W. Lau, John E. Hamer, Akira Hasebe   +5 more
doaj   +1 more source

Integrase-deficient lentiviral vectors mediate efficient gene transfer to human vascular smooth muscle cells with minimal genotoxic risk [PDF]

, 2012
We have previously shown that injury-induced neointima formation was rescued by adenoviral-Nogo-B gene delivery. Integrase-competent lentiviral vectors (ICLV) are efficient at gene delivery to vascular cells but present a risk of insertional mutagenesis.
Sessa, W.C., Chick, H.E., Kane, N.M., Baker, A.H., Thrasher, A.J., Alba, R., Fronza, R., McDonald, R.A., Delles, C., Schmidt, M., Nowrouzi, A.   +10 more
core   +1 more source

Integration Mapping of piggyBac-Mediated CD19 Chimeric Antigen Receptor T Cells Analyzed by Novel Tagmentation-Assisted PCR

EBioMedicine, 2018
Insertional mutagenesis is an important risk with all genetically modified cell therapies, including chimeric antigen receptor (CAR)-T cell therapy used for hematological malignancies.
Motoharu Hamada, Nobuhiro Nishio, Yusuke Okuno, Satoshi Suzuki, Nozomu Kawashima, Hideki Muramatsu, Shoma Tsubota, Matthew H. Wilson, Daisuke Morita, Shinsuke Kataoka, Daisuke Ichikawa, Norihiro Murakami, Rieko Taniguchi, Kyogo Suzuki, Daiei Kojima, Yuko Sekiya, Eri Nishikawa, Atsushi Narita, Asahito Hama, Seiji Kojima, Yozo Nakazawa, Yoshiyuki Takahashi   +21 more
doaj   +1 more source

AAV and Insertional Mutagenesis

Molecular Therapy, 2007
In this issue of Molecular Therapy, David Russell provides an invited commentary on data presented at the tenth annual meeting of the American Society of Gene Therapy that strongly suggested that adenovirus-associated virus (AAV) vector insertion could in certain models be linked to the development of cancer.1 A short report describing the data that ...
openaire   +2 more sources

Random insertional mutagenesis in fungal genomes to identify virulence factors

, 2011
Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in ...
Parthasarathy Santhanam, Santhanam, P.
core   +1 more source

Insertional mutagenesis in the zoonotic pathogen Chlamydia caviae.

PLoS ONE, 2019
The ability to introduce targeted genetic modifications in microbial genomes has revolutionized our ability to study the role and mode of action of individual bacterial virulence factors.
Kimberly Filcek, Katarina Vielfort, Samada Muraleedharan, Johan Henriksson, Raphael H Valdivia, Patrik M Bavoil, Barbara S Sixt   +6 more
doaj   +1 more source

Gene editing enables T-cell engineering to redirect antigen specificity for potent tumor rejection

Life Science Alliance, 2019
Targeted integration of a tumor-reactive T-cell receptor into the TRAC locus using CRISPR-Cas9 and AAV6 redirects primary human T cells against tumor cells in vitro and in vivo .
Julian J Albers, Tim Ammon, Dario Gosmann, Stefan Audehm, Silvia Thoene, Christof Winter, Ramona Secci, Anja Wolf, Anja Stelzl, Katja Steiger, Jürgen Ruland, Florian Bassermann, Christian Kupatt, Martina Anton, Angela M Krackhardt   +14 more
doaj   +1 more source