Results 71 to 80 of about 45,003 (206)

Morphophysiological alterations caused by insertional mutagenesis of contactin 5 (Cntn5) gene in transgenic mice

Вавиловский журнал генетики и селекции, 2017
Transgenesis has become a routine for modern biological studies. The most popular method for producing transgenic animals–pronuclear microinjection–frequently leads to host gene disruption due to a random transgene integration.
A. V. Smirnov, N. A. Feofanova, G. V. Kontsevaya, M. V. Anisimova, I. I. Kovrigin, I. A. Serova, M. P. Moshkin, L. A. Gerlinskaya, N. R. Battulin   +8 more
doaj   +1 more source

A PATO-compliant zebrafish screening database (MODB): management of morpholino knockdown screen information [PDF]

, 2008
Background The zebrafish is a powerful model vertebrate amenable to high throughput in vivo genetic analyses. Examples include reverse genetic screens using morpholino knockdown, expression-based screening using enhancer trapping and forward genetic ...
Michelle N Knowlton, Tongbin Li, Yongliang Ren, Brent R Bill, Lynda BM Ellis, Stephen C Ekker, CB Kimmel, R Geisler, BW Draper, E Wienholds, A Amsterdam, MA Pickart, MR Taylor, CE Eckfeldt, A Michael, EW Klee, VS Sadl, V Miller-Bertoglio, J Sprague, MA Bogue, T Horn, AA Rana, A Nasevicius, SF Altschul, A Amsterdam, A Amsterdam, R Dahm   +26 more
core   +1 more source

THE ROLE OF mutR GENE IN METABOLISM AND VIRULENCE OF emm12 GENOTYPE STREPTOCOCCUS PYOGENES STRAINS

Инфекция и иммунитет, 2015
In the present study the functional role of the mutR regulatory protein gene of Streptococcus pyogenes (emm12) was studied. The mutR gene was inactivated in the strains no. 97 and no. 152 by insertional mutagenesis.
A. A. Zutkis, B. L. Milman, A. V. Dmitriev   +2 more
doaj   +1 more source

A Unifying Scenario on the Origin and Evolution of Cellular and Viral Domains [PDF]

, 2009
The cellular theory on the nature of life has been one of the first major advancements in biology. Viruses, however, are the most abundant life forms, and their exclusion from mainstream biology and the Tree of Life (TOL) is a major paradox in biology ...
Claudiu I. Bandea
core   +1 more source

ATIDB: Arabidopsis thaliana insertion database [PDF]

, 2003
Insertional mutagenesis techniques, including transposon- and T-DNA-mediated mutagenesis, are key resources for systematic identification of gene function in the model plant species Arabidopsis thaliana.
Pan, X. K., Liu, H., Clarke, J., Jones, J., Bevan, M., Stein, L.   +5 more
core   +1 more source

CRIMP: a CRISPR/Cas9 insertional mutagenesis protocol and toolkit

Nature Communications
Site-directed insertion is a powerful approach for generating mutant alleles, but low efficiency and the need for customisation for each target has limited its application.
Lee B. Miles, Vanessa Calcinotto, Sara Oveissi, Rita J. Serrano, Carmen Sonntag, Orlen Mulia, Clara Lee, Robert J. Bryson-Richardson   +7 more
doaj   +1 more source

TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii.

PLoS ONE, 2020
Generation and subsequent analysis of mutants is critical to understanding the functions of genes and proteins. Here we describe TIM, an efficient, cost-effective, CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomonas ...
Tyler Picariello, Yuqing Hou, Tomohiro Kubo, Nathan A McNeill, Haru-Aki Yanagisawa, Toshiyuki Oda, George B Witman   +6 more
doaj   +1 more source

International Consortium of Rice Mutagenesis: Resources and beyond [PDF]

, 2013
Rice is one of the most important crops in the world. The rice community needs to cooperate and share efforts and resources so that we can understand the functions of rice genes, especially those with a role in important agronomical traits, for ...
Droc, Gaëtan, Guiderdoni, Emmanuel, Hsing, Yue-ie C., Wei, Fu-Jin   +3 more
core   +2 more sources

Transposon Insertion Mutagenesis for Archaeal Gene Discovery [PDF]

, 2016
Archaea constitute the third domain of life, but studies on their physiology and other features have lagged behind bacteria and eukarya, largely due to the challenging biology of archaea and concomitant difficulties in methods development. The use of genome-wide en masse insertion mutagenesis is one of the most efficient means to discover the genes ...
Kiljunen, Saija Johanna, Pajunen, Maria Irene, Savilahti, Harri Tapani   +2 more
openaire   +3 more sources

Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]

, 2018
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin, A Burt, A Hammond, AP Lorenzetti, B McClintock, BA Castilho, C Feschotte, C Feschotte, C Feschotte, C Kettlun, C Lu, C Ye, CN Hancock, DBT Cox, DJ Garfinkel, F Palazzoli, FA Ran, FJM Mojica, G Feng, G Mai, G Yang, H Kuang, H Mao, HT Dong, IK Jordan, J Hou, J Li, J Paszkowski, JA Doudna, JB Owens, JD Hollister, JE DiCarlo, JI Qüesta, JK Baillie, JM Casacuberta, K Kikuchi, K Naito, K Naito, K Shirasawa, KD Kim, KM Esvelt, KS Makarova, L Bortesi, L Cong, LC Li, LM Vaschetto, Luis María Vaschetto, M Cowley, M Jinek, M Momose, M Patel, M Szabo, M Turner, N Buchon, N Jiang, N Jiang, N Jiang, Q Zhang, R Barrangou, R Jansen, RM Walsh, SD Molyneux, SR Wessler, SR Yant, T Mourier, T Sakuma, T Singer, VM Gantz, W Matsunaga, WC Lai, X Li, X Li, X Lin, X Shan, Y Ishino, Y Yan, ZN Adelman   +76 more
core   +1 more source