Results 81 to 90 of about 45,003 (206)

Retrotransposon Tto1: functional analysis and engineering for insertional mutagenesis [PDF]

, 2011
Retrotransposons are genomic parasites activated by stress conditions that can be seriously detrimental for their host. In this work I demonstrate that Tto1, a typical plant LTR retrotransposon with insertion preference into genes can be turned into a ...
Tramontano, Andrea, Tramontano, Andrea, Tramontano, Andrea   +2 more
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Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants [PDF]

, 2011
A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based screenings that allow for identification ...
Catalanotti, Claudia, Fernández Reyes, Emilio, Galván Cejudo, Aurora, González-Ballester, David, Grossman, Arthur R., Higuera, José J., Magneschi, Leonardo, Montaigu, Amaury de, Mus, Florence, Pootakham, Wirulda, Prior, Matthew, Yang, Wenqiang   +11 more
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Sleeping Beauty Mouse Models of Cancer: Microenvironmental Influences on Cancer Genetics

Frontiers in Oncology, 2019
The Sleeping Beauty (SB) transposon insertional mutagenesis system offers a streamlined approach to identify genetic drivers of cancer. With a relatively random insertion profile, SB is uniquely positioned for conducting unbiased forward genetic screens.
Amy Guimaraes-Young, Charlotte R. Feddersen, Adam J. Dupuy   +2 more
doaj   +1 more source

In Vivo RNAi Screening Identifies Regulators of Actin Dynamics as Key Determinants of Lymphoma Progression [PDF]

, 2009
April 1, 2010Mouse models have markedly improved our understanding of cancer development and tumor biology. However, these models have shown limited efficacy as tractable systems for unbiased genetic experimentation.
A Besson, A Leask, AG Uren, AJ Dupuy, AJ Ridley, B Luo, Corbin E Meacham, DA Grueneberg, DJ Burgess, E Helfer, Emily E Ho, Esther Dubrovsky, Frank B Gertler, H Nishihara, J Jonkers, JA Cancelas, JA Nick, JM Adams, K Berns, K Burridge, L Zender, LG Smith, LS Collier, M Margulies, M van Lohuizen, Michael T Hemann, MR Schlabach, MT Hemann, N Tani, PN Kelly, R Firestein, RA Dickins, S Kleber, T Suzuki, TF Westbrook, VN Ngo, Y Gao   +36 more
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Analysis of tumor heterogeneity and cancer gene networks using deep sequencing of MMTV-induced mouse mammary tumors.

PLoS ONE, 2013
Cancer develops through a multistep process in which normal cells progress to malignant tumors via the evolution of their genomes as a result of the acquisition of mutations in cancer driver genes. The number, identity and mode of action of cancer driver
Christiaan Klijn, Marco J Koudijs, Jaap Kool, Jelle ten Hoeve, Mandy Boer, Joost de Moes, Waseem Akhtar, Martine van Miltenburg, Annabel Vendel-Zwaagstra, Marcel J T Reinders, David J Adams, Maarten van Lohuizen, John Hilkens, Lodewyk F A Wessels, Jos Jonkers   +14 more
doaj   +1 more source

A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites

, 2009
Insertional mutagens such as viruses and transposons are a useful tool for performing forward genetic screens in mice to discover cancer genes. These screens are most effective when performed using hundreds of mice, however until recently a major ...
A Ambrosi, AG Uren, AJ Dupuy, AM Bender, Anders H Lund, Anthony G Uren, Anton Berns, C Greenman, Catherine H Wilson, David J Adams, EK Hui, FA Giordano, GP Wang, H Mikkers, H Mikkers, H Ochman, Harald Mikkers, HT Cuypers, J Kong, Jaap Kool, JC Mullikin, Jos Jonkers, Louise van der Weyden, LS Collier, M Margulies, MA Marra, MA McAleer, Maarten van Lohuizen, OS Kustikova, P Parameswaran, PN Hengen, Richard Rance, RS Devon, VW Keng, X Wu   +34 more
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Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. [PDF]

, 2017
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost
ARAÚJO, W. L. de, FAVARO, L. C. de L., FERREIRA, A., PALLU, A. P. de S., PIZZIRANI-KLEINER, A. A., RODRIGUES, M. B. C., RODRIGUES, M. J. C., SEBASTIANES, F. de S., SPÓSITO, M. B.   +8 more
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Limiting Thymic Precursor Supply Increases the Risk of Lymphoid Malignancy in Murine X-Linked Severe Combined Immunodeficiency

Molecular Therapy: Nucleic Acids, 2017
In early gene therapy trials for SCID-X1, using γ-retroviral vectors, T cell leukemias developed in a subset of patients secondary to insertional proto-oncogene activation.
Samantha L. Ginn, Claus V. Hallwirth, Sophia H.Y. Liao, Erdahl T. Teber, Jonathan W. Arthur, Jianmin Wu, Hong Ching Lee, Szun S. Tay, Min Hu, Roger R. Reddel, Matthew P. McCormack, Adrian J. Thrasher, Marina Cavazzana, Stephen I. Alexander, Ian E. Alexander   +14 more
doaj   +1 more source

The Toxoplasma gondii active serine hydrolase 4 regulates parasite division and intravacuolar parasite architecture [PDF]

, 2018
Hydrolase are enzymes that regulate diverse biological processes, including posttranslational protein modifications. Recent work identified four active serine hydrolases (ASHs) in Toxoplasma gondii as candidate depalmitoylases. However, only TgPPT1 (ASH1)
Amara, Neri, Amberg-Johnson, Katherine, Beatty, Wandy, Bogyo, Matthew, Foe, Ian T, Garner, Rikki M, Onguka, Ouma, Yeh, Ellen   +7 more
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Food and feed safety aspects of cisgenic crop plant varieties [PDF]

This report presents the results of the discussions that identified food and feed safety aspects of cisgenic plant varieties in comparison to conventional varieties on the one hand and transgenic plant varieties on the other hand.
Kok, E.J., Prins, T.W.
core   +2 more sources