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Specific Determinants of the Transmembrane Region of the Andes Virus Gc Glycoprotein Drive the Transition from Membrane Hemifusion to Pore Formation. [PDF]
VirusesMárquez CL +3 more
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A CRISPR-Based Mutagenesis Strategy for Examining CLAG3 Helix 44 Contribution to Malaria Parasite Nutrient Uptake Channels. [PDF]
Genes (Basel)Gonzalez-Chavez Z +3 more
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Functional Characterization of the Histidine Kinase BaeS Reveals Critical Residues for BaeSR-Dependent Stress Signaling in <i>Escherichia coli</i>. [PDF]
MicroorganismsChen S +10 more
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Editor's Choice Protein engineering strategies to develop lectins by design. [PDF]
GlycobiologyHombu R, Beatty LE, Neelamegham S.
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A cold-adapted and detergent-stable α-amylase from marine bacterium Photobacterium gaetbulicola Gung47<sup>T</sup>. [PDF]
Appl Microbiol BiotechnolLi J +6 more
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Site-Directed Mutagenesis [PDF]
, 2010 The technique of site-directed mutagenesis has been used to characterize gene and protein structure-function relationships, protein-protein interactions, binding domains of proteins, or active sites of enzymes for the last three decades. In this technique, a nucleotide sequence of interest is experimentally altered using synthetic oligonucleotides. The
Carrigan, Patricia E. +2 more
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In Vitro Site Directed Mutagenesis
2023Site-Directed Mutagenesis (SDM) allows for changes in the DNA sequence of plasmids using polymerase chain reaction (PCR). It is a reliable, accessible, and rapid method which is the common initial step of many biochemial or genetic experiments. Here we describe the various different forms of SDM before giving a detailed method for the introduction of ...
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2013
Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational ...
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Site-directed mutagenesis is a PCR-based method to mutate specified nucleotides of a sequence within a plasmid vector. This technique allows one to study the relative importance of a particular amino acid for protein structure and function. Typical mutations are designed to disrupt or map protein-protein interactions, mimic or block posttranslational ...
openaire +3 more sources
1983
In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role.
B. Singer, D. Grunberger
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In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role.
B. Singer, D. Grunberger
openaire +1 more source