Results 181 to 190 of about 347,517 (297)

Site-Directed Mutagenesis

International Journal of Research Studies in Biosciences, 2017
openaire   +1 more source

Site-Directed Mutagenesis v1

This protocols describes the procedure for site-directed mutagenesis by PCR
openaire   +1 more source

Pyroptosis‐Inducing Engineered Microparticles for Cancer Immunotherapy

Advanced Science, EarlyView.
Engineered microparticles co‐delivering geldanamycin and dual nanobodies induce targeted pyroptosis and block PD‐L1 and CD47 pathways, reprogramming the tumor microenvironment and achieving potent antitumor immunity in lung cancer models with minimal toxicity.
Tianli Hao, Zihan Deng, Yufei Liu, Li Liu, Deqiang Deng, Muyang Yang, Yuanyuan Geng, Yao Sun, Beilei Yue, Jonathan F. Lovell, Yushuai Liu, Lisen Lu, Honglin Jin   +12 more
wiley   +1 more source

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, EarlyView.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

AI-Driven Enzyme Engineering: Emerging Models and Next-Generation Biotechnological Applications. [PDF]

Molecules
Khan MF, Khan MT.
europepmc   +1 more source

Evolutionary Assembly and Future Design of Gibberellin Signaling

Advanced Science, EarlyView.
ABSTRACT Gibberellin (GA) signaling is widely regarded as a canonical hormone‐controlled growth pathway, yet accumulating evidence suggests that it emerged through stepwise evolutionary assembly rather than as a pre‐formed regulatory system. Here, we revisit the origin and diversification of the GA pathway from an evolutionary perspective.
Weishu Fan, Xihan Chen, Dan Li, Xiuhua Gao, Xiangdong Fu   +4 more
wiley   +1 more source

Site-Directed Mutagenesis [PDF]

, 2008
openaire   +2 more sources

Amenability of the Gatekeeper Enzyme HphA to Engineering in the Homologation Pathway of l‑Phenylalanine and l‑Tyrosine through Homology-Based Site-Directed Mutagenesis. [PDF]

ACS Omega
Lang Harman RM, Blackstone HG, Aruna FO, Patel SR, Shin M, NeSmith RK, Dickson DB, Spencer AC, Mori S.   +8 more
europepmc   +1 more source

Machine Learning‐Enhanced Random Matrix Theory Design for Human Immunodeficiency Virus Vaccine Development

Advanced Intelligent Discovery, EarlyView.
This study integrates random matrix theory (RMT) and principal component analysis (PCA) to improve the identification of correlated regions in HIV protein sequences for vaccine design. PCA validation enhances the reliability of RMT‐derived correlations, particularly in small‐sample, high‐dimensional datasets, enabling more accurate detection of ...
Mariyam Siddiqah, Muhammad Zulqarnain Zeb, Muhammad Husnain Zeb, Babar Shabbir   +3 more
wiley   +1 more source

Multicyclic D‐Stereospecific Hydrolase Dimer With High Sustained Activity

Angewandte Chemie, EarlyView.
Protein macrocyclization was applied to a thermally unstable D‐stereospecific hydrolase using in situ cyclization of proteins (INCYPRO). This site‐specific cross‐linking approach enhanced the resistance of the enzyme to heat and cosolvents.
Anissa Haim, Sandra Liebscher, Rasmus Klintrot, Lorenzo Vallino, Marcelo Masman, Andreas H. Simon, Marianne Hahn, Sven Hennig, Saskia Neubacher, Frank Bordusa, Tom N. Grossmann   +10 more
wiley   +2 more sources