Results 181 to 190 of about 349,101 (299)

Smart-driven bioengineering techniques for enhancing microalgal biohydrogen production. [PDF]

Biotechnol Biofuels Bioprod
Sun Z, Guo S, Ugya AY, Cheng W, Zhang Y, Sun L.   +5 more
europepmc   +1 more source

SDS‐CRISPR for Single‐Nucleotide Variant Detection

Advanced Science, EarlyView.
Structure‐disruption‐sensitive CRISPR (SDS‐CRISPR) converts structural instability into single‐nucleotide precision, thereby overcoming mismatch tolerance in canonical Cas12a and enabling versatile diagnostics across DNA, RNA, and microRNA targets. When applied to rapid IDH1 mutation detection for glioma genotyping and integrated with lateral‐flow ...
Xin Guan, Chong Guo, Jiongyu Zhang, Rui Yang, Yerramsetti Ramachandra, Chengyu Hou, Minjie Pei, Shuo Zhang, Kurt T. Schalper, Xingye Liu, Qian Wu, Ketan R. Bulsara, Changchun Liu   +12 more
wiley   +1 more source

Site-Directed Mutagenesis [PDF]

, 2008
openaire   +2 more sources

Phage-assisted evolution of allosteric protein switches. [PDF]

Nat Commun
Southern NT, von Bachmann A, Hovsepyan A, Griebl M, Wolf B, Lemmen N, Kroell AS, Westermann S, Mathony J, Niopek D.   +9 more
europepmc   +1 more source

Fosl2 Regulates FSH‐Dependent Follicle Maturation Through Feedback Amplification of FSH/FSHR Signaling

Advanced Science, EarlyView.
This study identifies a FOSL2‐driven positive feedback loop that amplifies FSH/FSHR signaling. During FSH‐dependent follicle maturation, FSH induces Fosl2 expression via the cAMP‐PKA‐CREB cascade. FOSL2 in turn binds the promoters of Fshr and estrogen‐biosynthesis genes to enhance their transcription, thereby increasing Fshr mRNA level and amplifying ...
Hongru Shi, Chaoli Chen, Zaohong Ran, Jianning Liao, Zian Wu, Xiaodong Wang, Yongheng Zhao, Wenkai Ke, Bowen Tan, Yun Liu, Youqiang Su, Wei Ren, Xiang Li, Changjiu He   +13 more
wiley   +1 more source

A Protocol for Regulating Protein Liquid-Liquid Phase Separation Using NMR-Guided Mutagenesis. [PDF]

Methods Protoc
Enomoto-Kusano M, Furuita K, Kodama TS, Kojima C.   +3 more
europepmc   +1 more source

Microbial Odorant Detection Guides Drosophila Parasitoids Seeking Hosts in Fermenting Fruits

Advanced Science, EarlyView.
Yeast microbes in fermenting fruits attract both host flies and their parasitoid wasps. Female Leptopilina boulardi detect yeast‐emitted ethyl esters via two olfactory receptors, LbouOR167 and LbouOR136. A conserved residue, Leu159, is critical for binding these compounds, enabling female wasps to locate host‐rich habitats.
Yueqi Lu, Lan Pang, Wenqi Shi, Ting Feng, Zhi Dong, Yifeng Sheng, Sicong Zhou, Longtao Yu, Hao Guo, Ying Wang, Jiani Chen, Jianhua Huang   +11 more
wiley   +1 more source

Editorial: Mutational breeding: from induced mutations to site-directed mutagenesis

Frontiers in Plant Science
Søren K. Rasmussen, Shri Mohan Jain
doaj   +1 more source

Engineering Compact Base Editors by AlphaFold-Guided Mutation Scan and Escherichia coli-Based Tri-Selection. [PDF]

Adv Sci (Weinh)
Gang Son R, Kim G, Lee JK.
europepmc   +1 more source

Site-Directed Mutagenesis v1

This protocols describes the procedure for site-directed mutagenesis by PCR
openaire   +1 more source