Results 111 to 120 of about 470,712 (310)
Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan +7 more
wiley +1 more source
Polytetrafluoroethylene utensils as a source of micro- and nanoplastics: Insights from a
Eco-Environment & HealthPolytetrafluoroethylene (PTFE), widely used in household products and cookware, can release particles into food, posing potential health risks. The biological interactions of the resulting micro- and nanoplastics (MNPLs) remain poorly understood but can ...
Mohamed Alaraby +6 more
doaj +1 more source
Somatic and germline mutagenesis assayed by the unstable zeste-white test in Drosophila melanogaster
, 1992 This investigation is an attempt to compare mutation rates in germinal and somatic cells by the use of the unstable zeste-white assay in Drosophila melanogaster.
Larsson, Jan,, Rasmuson, Åsa,
core
Mutagenesis primers used in this study.
, 2020 Mutagenesis primers used in this study.
Sarah Wettstadt (7107035) +1 more
core +1 more source
Advanced Functional Materials, EarlyView.Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou +6 more
wiley +1 more source
Cas9/sgRNA-mediated mutagenesis in Arabidopsis.
, 2014 Scheme for Cas9/sgRNA-mediated mutagenesis of a non-functional (out-of-frame) mutant GFP gene, detection of T1 Arabidopsis leaves with restored GFP gene function by confocal microscopy and documentation of target site DNA mutagenesis.
WenZhi Jiang (576280) +2 more
core +1 more source
Engineering Antigen Density on M13 Bacteriophage for Personalized Nanovaccine
Advanced Healthcare Materials, EarlyView.Increasing antigen density on the phage capsid impacts phage uptake and antigen cross‐presentation by antigen‐presenting cells, leading to enhanced activation of antitumor CD8+ T cells in the draining lymph nodes. These activated CD8+ T cells subsequently recognize the antigen‐expressing tumors and mediate their specific cytotoxic killing, thereby ...
Shengnan Huang +7 more
wiley +1 more source
Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering
Advanced Materials, EarlyView.Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera +22 more
wiley +1 more source
Searching for sequence directed mutagenesis in eukaryotes.
, 2007 Sequence directed mutagenesis is a mechanism by which imperfect repeats ¿repair¿ each other to become perfect, generating mutations. This process is known to be prevalent in prokaryotes and it has been implicated in several human genetic diseases.
Emmanuel D Ladoukakis (16100591) +1 more
core
Mutagenesis to Study Channel Structure
, 2001 Recently the crystal structure of a potassium channel has been determined (1). The emerging structural features turned out to be a beautiful illustration of both the power and limitation of the approach of structure—function analysis by mutagenesis.
Dahl, Gerhard +3 more
core +1 more source