Results 11 to 20 of about 291 (106)

Power analyses to inform Duplex Sequencing study designs for MutaMouse liver and bone marrow

Environmental and Molecular Mutagenesis
AbstractRegulatory genetic toxicology testing is essential for identifying potentially mutagenic hazards. Duplex Sequencing (DS) is an error‐corrected next‐generation sequencing technology that provides substantial advantages for mutation analysis over conventional mutagenicity assays including: improved accuracy of mutation detection, ability to ...
Elena Esina, Annette E. Dodge, Andrew Williams, David M. Schuster, Danielle P. M. LeBlanc, Francesco Marchetti, Carole L. Yauk   +6 more
openaire   +3 more sources

Genotoxicity of 3-nitrobenzanthrone and 3-aminobenzanthrone in Muta™Mouse and lung epithelial cells derived from Muta™Mouse [PDF]

Mutagenesis, 2008
FE1 lung epithelial cells derived from MutaMouse are a new model system to provide in vitro mutagenicity data with the potential to predict the outcome of an in vivo MutaMouse test.
Arlt, Volker M, Douglas, George R, Gingerich, John, Phillips, David H, Schmeiser, Heinz H, White, Paul A   +5 more
core   +9 more sources

Benchmark Response (BMR) Values for In Vivo Mutagenicity Endpoints [PDF]

Environmental and Molecular Mutagenesis, Volume 66, Issue 4, Page 172-184, April 2025.
The benchmark dose (BMD) approach constitutes the most effective and pragmatic strategy for the derivation of a point of departure (PoD) for comparative potency analysis, risk assessment, and regulatory decision‐making.
George Johnson
core   +2 more sources

Increased mutagenicity in the liver of MutaMouse females following oral treatment with commercial-grade toluene diisocyanate

Genes and Environment
Background Toluene diisocyanates (TDIs) are high-production-volume chemicals widely used in polyurethane manufacturing. A typical commercial-grade TDI (TDI; 2,4-toluene diisocyanate: 2,6-toluene diisocyanate; 80:20), CAS: 26471-62-5, is mutagenic in ...
Mariko Matsumoto, Masakatsu Natsume, Takako Iso, Takaaki Umano, Yasumasa Murata, Kenichi Masumura, Katsuyoshi Horibata, Kei-ichi Sugiyama   +7 more
doaj   +2 more sources

The transgenic MutaMouse hepatocyte mutation assay in vitro: Mutagenicity and mutation spectra of six substances with different mutagenic mechanisms [PDF]

Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Mutagenicity testing is a component of the hazard assessment of industrial chemicals, biocides, and pesticides. Mutations induced by test substances can be detected by in vitro and in vivo methods that have been adopted as OECD Test Guidelines.
Dammann, Martina, Eichenlaub, Michael, Funk-Weyer, Dorothee, Göpfert, Alina, Honarvar, Naveed, Landsiedel, Robert, Rülker, Claudia, Schuster, David M., Tokovenko, Bogdan   +8 more
core   +4 more sources

Transcriptional profiling of dibenzo[def, p]chrysene-induced spleen atrophy provides mechanistic insights into its immunotoxicity in mutamouse [PDF]

Toxicological Sciences, 2015
Dibenzo[def, p]chrysene (DBC) is the most carcinogenic polycyclic aromatic hydrocarbon (PAH) examined to date. We investigated the immunotoxicity of DBC, manifested as spleen atrophy, following acute exposure of adult MutaMouse males by oral gavage. Mice
Arlt, Volker M., Chepelev, Nikolai L., Gagné, Rémi, Kennedy, Dean A., Kuo, Byron, Long, Alexandra S., Phillips, David H., White, Paul A., Williams, Andrew, Yauk, Carole L.   +9 more
core   +8 more sources

Benchmark dose analyses of multiple genetic toxicity endpoints permit robust, cross-tissue comparisons of MutaMouse responses to orally delivered benzo[a]pyrene [PDF]

Archives of Toxicology, 2017
Genetic damage is a key event in tumorigenesis, and chemically induced genotoxic effects are a human health concern. Although genetic toxicity data have historically been interpreted using a qualitative screen-and-bin approach, there is increasing ...
Alexandra S. Long, Dorothy Krolak, John W. Wills, Matthew Guo, Paul A. White, Stephen D. Dertinger, Volker M. Arlt   +6 more
core   +8 more sources

The In Vitro Transgenic Rodent Assay in Primary MutaMouse Hepatocytes Compared to the Mammalian Cell Gene Mutation Assay Using the HPRT Gene

Environmental and Molecular Mutagenesis
ABSTRACT Gene mutations can be detected in mammalian cells in vitro using indicator genes such as the hypoxanthine‐guanine‐phosphoribosyltransferase (HPRT) gene. These assays have been adopted as OECD test guidelines (TG, e.g., OECD TG no. 476) and are used for regulatory purposes.
Alina Goepfert, Kylee Ronnenberg, Claudia Ruelker, Silke Spang, Naveed Honarvar, Robert Landsiedel   +5 more
openaire   +2 more sources

Chemically induced mutations in a MutaMouse reporter gene inform mechanisms underlying human cancer mutational signatures [PDF]

Communications Biology, 2020
Abstract Transgenic rodent (TGR) models use bacterial reporter genes to quantify in vivo mutagenesis. Pairing TGR assays with next-generation sequencing (NGS) enables comprehensive mutation pattern analysis to inform mutational mechanisms. We used this approach to identify 2751 independent lacZ
Marc A. Beal, Matthew J. Meier, Danielle P. LeBlanc, Clotilde Maurice, Jason M. O’Brien, Carole L. Yauk, Francesco Marchetti   +6 more
openaire   +2 more sources

Folate deficiency increases chromosomal damage and mutations in hematopoietic cells in the transgenic mutamouse model [PDF]

Environmental and Molecular Mutagenesis, 2018
Folate deficiency causes megaloblastic anemia and neural tube defects, and is also associated with some cancers. In vitro, folate deficiency increases mutation frequency and genome instability, as well as exacerbates the mutagenic potential of known environmental mutagens.
Danielle P, LeBlanc, Nathalie A, Behan, Jason M, O'Brien, Francesco, Marchetti, Amanda J, MacFarlane   +4 more
openaire   +2 more sources