Integrated In Vivo Genotoxicity Assessment of Procarbazine Hydrochloride Demonstrates Induction of Pig-a and LacZ Mutations, and Micronuclei, in MutaMouse Hematopoietic Cells. [PDF]
Environ Mol Mutagen, 2019 Procarbazine hydrochloride (PCH) is a DNA‐reactive hematopoietic carcinogen with potent and well‐characterized clastogenic activity. However, there is a paucity of in vivo mutagenesis data for PCH, and in vitro assays often fail to detect the genotoxic effects of PCH due to the complexity of its metabolic activation. We comprehensively evaluated the in
Maurice C +3 more
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Validation of an In Vitro Mutagenicity Assay Based on Pulmonary Epithelial Cells from the Transgenic MutaMouse: Intra-Laboratory Variability and Metabolic Competence [PDF]
, 2018 Genetic toxicity tests used for regulatory screening must be rigorously validated to ensure accuracy, reliability and relevance. Hence, prior to establishment of an internationally-accepted test guideline, a new assay must undergo multi-stage validation.
Hanna, Joleen
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Diesel exhaust particles are mutagenic in FE1-Muta™Mouse lung epithelial cells
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 2008 The particulate phase of diesel engine exhaust is likely carcinogenic. However, the mechanisms of diesel exhaust particles (DEPs) induced mutagenicity/carcinogenicity are still largely unknown. We determined the mutant frequency following eight repeated 72 h incubations with 37.5 or 75 microg/ml DEP (NIST SRM 1650) in the FE1-MutaMouse lung epithelial ...
Jacobsen, Nicklas Raun +5 more
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The Genetic Toxicity of Polycyclic Aromatic Hydrocarbons: A Cross-Tissue, Multi-Endpoint Study in the Transgenic MutaMouse [PDF]
, 2017 Polycyclic aromatic hydrocarbons (PAHs) are produced via the incomplete combustion of organic matter. They are ubiquitously present in the environment, and human exposures typically involve complex PAH mixtures in complex matrices (e.g., soil, urban air).
Long, Alexandra
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, 2023 Additional file 1: Supplementary Table 1. Clinical observations in the gene mutation assay of tert-butyl hydroperoxide. Supplementary Table 2. Historical data of negative controls (Transgenic rodent gene mutation assay (lacZ assay)).
Murata, Yasumasa +10 more
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Benchmark Response Values for Error-Corrected Sequencing Mutagenicity Assessment Technologies. [PDF]
Environ Mol MutagenABSTRACT The Benchmark Dose (BMD) approach is commonly used to determine Point‐of‐Departure (PoD) values for risk assessment and regulatory decision‐making; however, choosing a suitable Benchmark Response (BMR) for continuous endpoints is a challenge.
Mulugeta S +5 more
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Environmental and Molecular Mutagenesis, 2017 The screening of chemicals for the protection of human health and the environment requires the assessment of genetic toxicity. However, existing, internationally‐accepted in vitro mammalian genotoxicity tests have been criticized for their low specificity (i.e.
Rebecca M, Maertens +2 more
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Evaluation of Type 1 Error Rates in Duplex Sequencing for Mutagenicity Testing Using Vehicle Control Data and Simulation Analyses. [PDF]
Environ Mol MutagenABSTRACT Accurate mutation detection and quantification are crucial for understanding mutagenesis and its potential health implications. Traditional in vivo mutagenicity assays, such as the transgenic rodent gene mutation assay, are limited by their focus on single reporter genes and inability to efficiently generate mutation spectra.
Williams A +6 more
europepmc +2 more sources
Chemically induced mutations in a MutaMouse reporter gene inform mechanisms underlying human cancer mutational signatures. [PDF]
Commun Biol, 2020 Abstract Transgenic rodent (TGR) models use bacterial reporter genes to quantify in vivo mutagenesis. Pairing TGR assays with next-generation sequencing (NGS) enables comprehensive mutation pattern analysis to inform mutational mechanisms. We used this approach to identify 2751 independent lacZ
Beal MA +6 more
europepmc +4 more sources
Toxicity and Mutagenicity of Polycyclic Aromatic Hydrocarbons in Contaminated Soils using Mammalian In Vitro Assay [PDF]
There exists a growing need to establish a better in vitro model for evaluating PAH toxicity and carcinogenicity and to improve assessments of risk to human health, especially to the complex mixtures that exist in the environment.
Luker, George W.
core +12 more sources