Results 11 to 20 of about 260 (125)

In vitro mutagenicity of selected environmental carcinogens and their metabolites in MutaMouse FE1 lung epithelial cells. [PDF]

Mutagenesis, 2020
Abstract Chemicals in commerce or under development must be assessed for genotoxicity; assessment is generally conducted using validated assays (e.g. Tk mouse lymphoma assay) as part of a regulatory process. Currently, the MutaMouse FE1 cell mutagenicity assay is undergoing validation for eventual use as a standard in vitro mammalian ...
Hölzl-Armstrong L, Nævisdal A, Cox JA, Long AS, Chepelev NL, Phillips DH, White PA, Arlt VM.   +7 more
europepmc   +6 more sources

Duplex sequencing provides detailed characterization of mutation frequencies and spectra in the bone marrow of MutaMouse males exposed to procarbazine hydrochloride. [PDF]

Arch Toxicol, 2023
Abstract Mutagenicity testing is an essential component of health safety assessment. Duplex Sequencing (DS), an emerging high-accuracy DNA sequencing technology, may provide substantial advantages over conventional mutagenicity assays.
Dodge AE, LeBlanc DPM, Zhou G, Williams A, Meier MJ, Van P, Lo FY, Valentine Iii CC, Salk JJ, Yauk CL, Marchetti F.   +10 more
europepmc   +4 more sources

The In Vitro Transgenic Rodent Assay in Primary MutaMouse Hepatocytes Compared to the Mammalian Cell Gene Mutation Assay Using the HPRT Gene. [PDF]

Environ Mol Mutagen
ABSTRACT Gene mutations can be detected in mammalian cells in vitro using indicator genes such as the hypoxanthine‐guanine‐phosphoribosyltransferase (HPRT) gene. These assays have been adopted as OECD test guidelines (TG, e.g., OECD TG no. 476) and are used for regulatory purposes.
Göpfert A, Ronnenberg KKM, Ruelker C, Spang S, Honarvar N, Landsiedel R.   +5 more
europepmc   +4 more sources

The effect of dietary folic acid intake on somatic and germ cell mutations in the transgenic MutaMouse model [PDF]

, 2018
Folate is an essential water soluble B-vitamin required for DNA synthesis and methylation. Folate deficiency has been associated with some cancers, male subfertility, and causes megaloblastic anemia and neural tube defects. Using a transgenic mouse model
LeBlanc, Danielle
openaire   +3 more sources

Transcriptional Profiling of Dibenzo[def,p]chrysene-induced Spleen Atrophy Provides Mechanistic Insights into its Immunotoxicity in MutaMouse [PDF]

Toxicological Sciences, 2015
Dibenzo[def,p]chrysene (DBC) is the most carcinogenic polycyclic aromatic hydrocarbon (PAH) examined to date. We investigated the immunotoxicity of DBC, manifested as spleen atrophy, following acute exposure of adult MutaMouse males by oral gavage. Mice were exposed to 0, 2.0, 6.2, or 20.0 mg DBC /kg-bw per day, for 3 days. Genotoxic endpoints (DBC-DNA
Chepelev, Nikolai L., Long, Alexandra S., Williams, Andrew, Kuo, Byron, Gagné, Rémi, Kennedy, Dean A., Phillips, David H., Arlt, Volker M., White, Paul A., Yauk, Carole L.   +9 more
openaire   +6 more sources

The transgenic MutaMouse hepatocyte mutation assay in vitro: Mutagenicity and mutation spectra of six substances with different mutagenic mechanisms [PDF]

Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Mutagenicity testing is a component of the hazard assessment of industrial chemicals, biocides, and pesticides. Mutations induced by test substances can be detected by in vitro and in vivo methods that have been adopted as OECD Test Guidelines. One of these in vivo methods is the Transgenic Rodent Assay (TGRA), OECD test guideline no. 488. An analogous
Alina, Göpfert, David M, Schuster, Claudia, Rülker, Michael, Eichenlaub, Bogdan, Tokovenko, Martina, Dammann, Dorothee, Funk-Weyer, Naveed, Honarvar, Robert, Landsiedel   +8 more
openaire   +3 more sources

Genotoxicity of 3-nitrobenzanthrone and 3-aminobenzanthrone in Muta™Mouse and lung epithelial cells derived from Muta™Mouse [PDF]

Mutagenesis, 2008
FE1 lung epithelial cells derived from MutaMouse are a new model system to provide in vitro mutagenicity data with the potential to predict the outcome of an in vivo MutaMouse test. 3-Nitrobenzanthrone (3-NBA) is a potent mutagen and suspected human carcinogen identified in diesel exhaust and urban air pollution.
Arlt, Volker M, Gingerich, John, Schmeiser, Heinz H, Phillips, David H, Douglas, George R, White, Paul A   +5 more
openaire   +6 more sources

Power analyses to inform Duplex Sequencing study designs for MutaMouse liver and bone marrow

Environmental and Molecular Mutagenesis, Volume 65, Issue 8, Page 234-242, October 2024.
Abstract Regulatory genetic toxicology testing is essential for identifying potentially mutagenic hazards. Duplex Sequencing (DS) is an error‐corrected next‐generation sequencing technology that provides substantial advantages for mutation analysis over conventional mutagenicity assays including: improved accuracy of mutation detection, ability to ...
Elena Esina, Annette E. Dodge, Andrew Williams, David M. Schuster, Danielle P. M. LeBlanc, Francesco Marchetti, Carole L. Yauk   +6 more
wiley   +3 more sources

Additional file 1 of In vivo mutagenicity assessment of styrene in MutaMouse liver and lung

, 2023
Additional file 1. Supporting information table 1 data from previous controls (transgenic rodent gene mutation assay (lacz assay)
Murata, Yasumasa, Natsume, Masakatsu, Iso, Takako, Shigeta, Yoshiyuki, Hirose, Nozomu, Umano, Takaaki, Horibata, Katsuyoshi, Sugiyama, Kei-ichi, Masumura, Kenichi, Hirose, Akihiko, Matsumoto, Mariko   +10 more
openaire   +2 more sources

Benchmark dose analyses of multiple genetic toxicity endpoints permit robust, cross-tissue comparisons of MutaMouse responses to orally delivered benzo[a]pyrene. [PDF]

Arch Toxicol, 2018
Genetic damage is a key event in tumorigenesis, and chemically induced genotoxic effects are a human health concern. Although genetic toxicity data have historically been interpreted using a qualitative screen-and-bin approach, there is increasing interest in quantitative analysis of genetic toxicity dose-response data.
Long AS, Wills JW, Krolak D, Guo M, Dertinger SD, Arlt VM, White PA.   +6 more
europepmc   +9 more sources