Results 31 to 40 of about 291 (106)

Sequencing Chemically Induced Mutations in the Mutamouse Lacz Reporter Gene Identifies Human Cancer Mutational Signatures [PDF]

, 2019
ABSTRACTTransgenic rodent (TGR) models use bacterial reporter genes to quantifyin vivomutagenesis. Pairing TGR assays with next-generation sequencing (NGS) enables comprehensive mutation spectrum analysis to inform mutational mechanisms. We used this approach to identify 2,751 independentlacZmutations in the bone marrow of MutaMouse animals exposed to ...
Beal, Marc A., Meier, Matt J., LeBlanc, Danielle, Maurice, Clotilde, O’Brien, Jason, Yauk, Carole L., Marchetti, Francesco   +6 more
openaire   +1 more source

Risk assessment of N‐nitrosamines in food

EFSA Journal, Volume 21, Issue 3, March 2023., 2023
Abstract EFSA was asked for a scientific opinion on the risks to public health related to the presence of N‐nitrosamines (N‐NAs) in food. The risk assessment was confined to those 10 carcinogenic N‐NAs occurring in food (TCNAs), i.e. NDMA, NMEA, NDEA, NDPA, NDBA, NMA, NSAR, NMOR, NPIP and NPYR.
EFSA Panel on Contaminants in the Food Chain (EFSA CONTAM Panel), Dieter Schrenk, Margherita Bignami, Laurent Bodin, James Kevin Chipman, Jesús del Mazo, Christer Hogstrand, Laurentius (Ron) Hoogenboom, Jean‐Charles Leblanc, Carlo Stefano Nebbia, Elsa Nielsen, Evangelia Ntzani, Annette Petersen, Salomon Sand, Tanja Schwerdtle, Christiane Vleminckx, Heather Wallace, Benigni Romualdo, Cristina Fortes, Stephen Hecht, Marco Iammarino, Olaf Mosbach‐Schulz, Francesca Riolo, Anna Christodoulidou, Bettina Grasl‐Kraupp   +24 more
wiley   +1 more source

Additional file 1 of In vivo mutagenicity assessment of styrene in MutaMouse liver and lung

, 2023
Additional file 1. Supporting information table 1 data from previous controls (transgenic rodent gene mutation assay (lacz assay)
Murata, Yasumasa, Natsume, Masakatsu, Iso, Takako, Shigeta, Yoshiyuki, Hirose, Nozomu, Umano, Takaaki, Horibata, Katsuyoshi, Sugiyama, Kei-ichi, Masumura, Kenichi, Hirose, Akihiko, Matsumoto, Mariko   +10 more
openaire   +1 more source

Duplex sequencing provides detailed characterization of mutation frequencies and spectra in the bone marrow of MutaMouse males exposed to procarbazine hydrochloride

Archives of Toxicology, 2023
Abstract Mutagenicity testing is an essential component of health safety assessment. Duplex Sequencing (DS), an emerging high-accuracy DNA sequencing technology, may provide substantial advantages over conventional mutagenicity assays.
Annette E. Dodge, Danielle P.M. LeBlanc, Gu Zhou, Andrew Williams, Matthew J. Meier, Phu Van, Fang Yin Lo, Charles C. Valentine, Jesse J. Salk, Carole L. Yauk, Francesco Marchetti   +10 more
openaire   +2 more sources

Transcriptomic Analysis Reveals Novel Mechanistic Insight into Murine Biological Responses to Multi-Walled Carbon Nanotubes in Lungs and Cultured Lung Epithelial Cells [PDF]

, 2013
There is great interest in substituting animal work with in vitro experimentation in human health risk assessment; however, there are only few comparisons of in vitro and in vivo biological responses to engineered nanomaterials.
A Nel, A Takagi, A Takagi, AE John, AG Nasibulin, Andrew Williams, Anne T. Saber, AR Reddy, AT Saber, AT Saber, AT Saber, AT Saber, B Button, B Han, BN Snyder-Talkington, CA Poland, Carole L. Yauk, Carsten Købler, CC Chou, CL Yauk, CM Sayes, CM Sayes, DB Warheit, Dongmei Wu, DW Porter, Gayle E. Woloschak, GJ Gleich, H Wu, HJ Johnston, HJ Johnston, Håkan Wallin, IC Davis, IC Davis, J Pauluhn, J Pauluhn, J Seagrave, JA Bourdon, JA Bourdon, Jesper P. Bøgelund, JH Goodnight, JH Han, JY Choi, K Aschberger, K Yamashita, KE Wolk, Kristian Mølhave, KS Echtay, KS Echtay, L Ma-Hock, LA Mitchell, M Horie, M Husain, MA Maurer-Jones, Mainul Husain, MK Kerr, MK Kerr, MK Kerr, ML Berndt-Weis, MS Dresselhaus, MV Park, N Jacobsen, Nicklas R. Jacobsen, NR Jacobsen, NR Jacobsen, NR Jacobsen, Ole Andersen, P Jackson, PA White, Q Ma, RM Maertens, RR Mercer, S Halappanavar, Sabina Halappanavar, Sarah Labib, Sarah Søs Poulsen, SR Searle, Ulla Vogel, VM Arlt, X Cui, X Han, X Wang, X Wang, Y Benjamini, YH Yang, Zdenka O. Kyjovska   +84 more
core   +3 more sources

The mutagenic activity of select azo compounds in MutaMouse target tissues in vivo and primary hepatocytes in vitro

Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2019
The mutagenicity of Direct Black 38, Sudan I, and Para Red were evaluated in the in vivo MutaMouse assay and the in vitro MutaMouse primary hepatocyte (PH) assay. Direct Black 38 is an International Agency for Research on Cancer (IARC) Group 1 carcinogen and a prototypical benzidine-based azo compound that requires azo-reduction to yield a DNA-reactive
Julie A, Cox, Paul A, White
openaire   +2 more sources

Epidermal PPARγ influences subcutaneous tumor growth and acts through TNF-α to regulate contact hypersensitivity and the acute photoresponse [PDF]

, 2017
It is known that ultraviolet B (UVB) induces PPARγ ligand formation while loss of murine epidermal PPARγ (Pparg-/-epi) promotes UVB-induced apoptosis, inflammation, and carcinogenesis. PPARγ is known to suppress tumor necrosis factor-α (TNF-α) production.
Derr-Yellin, Ethel, Konger, Raymond L., Ocana, Jesus A., Sahu, Ravi P., Travers, Jeffrey B.   +4 more
core   +1 more source

The development and prevalidation of an in vitro mutagenicity assay based on MutaMouse primary hepatocytes, Part I: Isolation, structural, genetic, and biochemical characterization [PDF]

Environmental and Molecular Mutagenesis, 2018
To develop an improved in vitro mammalian cell gene mutation assay, it is imperative to address the known deficiencies associated with existing assays. Primary hepatocytes isolated from the MutaMouse are ideal for an in vitro gene mutation assay due to their metabolic competence, their “normal” karyotype (i.e., neither transformed nor immortalized ...
Cox, JA, Zwart, EP, Luijten, M, White, PA   +3 more
openaire   +4 more sources

Genetic analysis of PHIP intestinal mutations in Muta^TMMouse [PDF]

Mutagenesis, 1998
The mutagenicity of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) was investigated in male MutaMouse mice administered 20 mg/kg per o.s. for 4 days and killed 7 days later. Genomic DNA was extracted from liver, kidney and small and large intestine and the mutation frequency (MF) at the lacZ locus was determined using a positive selection assay.
A M, Lynch, N J, Gooderham, D S, Davies, A R, Boobis   +3 more
openaire   +2 more sources

Benzo[a]pyrene-induced DNA adducts and gene expression profiles in target and non-target organs for carcinogenesis in mice [PDF]

, 2014
Background: Gene expression changes induced by carcinogens may identify differences in molecular function between target and non-target organs. Target organs for benzo[a]pyrene (BaP) carcinogenicity in mice (lung, spleen and forestomach) and three non ...
Arlt, Volker M, Brewer, Daniel, Cooper, Colin, Phillips, David H, Zuo, Jie   +4 more
core   +3 more sources