Results 41 to 50 of about 291 (106)

The impact of p53 function on the metabolic activation of the carcinogenic air pollutant 3-nitrobenzanthrone and its metabolites 3-aminobenzanthrone and N-hydroxy-3-aminobenzanthrone in human cells [PDF]

, 2018
The tumour suppressor p53, encoded by TP53, is a key player in a wide network of signalling pathways. We investigated its role in the bioactivation of the environmental carcinogen 3-nitrobenzanthrone (3-NBA)found in diesel exhaust and its metabolites 3 ...
Arlt, Volker M, Baranski, Ann-Christin, Krais, Annette M, Phillips, David H, Schmeiser, Heinz H, Wohak, Laura E   +5 more
core   +2 more sources

Whole Genome Sequencing of the Mutamouse Model Reveals Strain- and Colony-Level Variation, and Genomic Features of the Transgene Integration Site [PDF]

Scientific Reports, 2019
AbstractThe MutaMouse transgenic rodent model is widely used for assessing in vivo mutagenicity. Here, we report the characterization of MutaMouse’s whole genome sequence and its genetic variants compared to the C57BL/6 reference genome. High coverage (>50X) next-generation sequencing (NGS) of whole genomes from multiple MutaMouse animals from the ...
Matthew J. Meier, Marc A. Beal, Andrew Schoenrock, Carole L. Yauk, Francesco Marchetti   +4 more
openaire   +2 more sources

Severity of effect considerations regarding the use of mutation as a toxicological endpoint for risk assessment: A report from the 8th International Workshop on Genotoxicity Testing (IWGT)

Environmental and Molecular Mutagenesis, EarlyView.
Abstract Exposure levels without appreciable human health risk may be determined by dividing a point of departure on a dose–response curve (e.g., benchmark dose) by a composite adjustment factor (AF). An “effect severity” AF (ESAF) is employed in some regulatory contexts.
Barbara L. Parsons, Marc A. Beal, Kerry L. Dearfield, George R. Douglas, Min Gi, B. Bhaskar Gollapudi, Robert H. Heflich, Katsuyoshi Horibata, Michelle Kenyon, Alexandra S. Long, David P. Lovell, Anthony M. Lynch, Meagan B. Myers, Stefan Pfuhler, Alisa Vespa, Andreas Zeller, George E. Johnson, Paul A. White   +17 more
wiley   +1 more source

Role of human aldo-keto reductases in the metabolic activation of the carcinogenic air pollutant 3-nitrobenzanthrone [PDF]

, 2018
3-Nitrobenzanthrone (3-NBA) is a potent mutagen and suspected human carcinogen detected in diesel exhaust particulate and ambient air pollution. It requires metabolic activation via nitroreduction to promote DNA adduct formation and tumorigenesis.
Albrecht Seidel, Arlt V. M., Burczynski M. E., Ciaccio P. J., Ciaccio P. J., Clementina A. Mesaros, Hsu N. Y., Ian A. Blair, IARC, Jessica R. Murray, Palmer G., Penning T. M., Smithgall T. E., Smithgall T. E., Straif K., Trevor M. Penning, Volker M. Arlt, Watts R. W. E.   +17 more
core   +2 more sources

The development and prevalidation of anin vitromutagenicity assay based on MutaMouse primary hepatocytes, Part II: Assay performance for the identification of mutagenic chemicals [PDF]

Environmental and Molecular Mutagenesis, 2019
As demonstrated in Part I, cultured MutaMouse primary hepatocytes (PHs) are suitable cells for use in anin vitrogene mutation assay due to their metabolic competence, their “normal” phenotype, and the presence of the MutaMouse transgene for reliable mutation scoring.
Cox, JA, Zwart, EP, Luijten, M, White, PA   +3 more
openaire   +4 more sources

Transferability and Reproducibility of the HepaRG CometChip Assay

Environmental and Molecular Mutagenesis, Volume 66, Issue 9, Page 478-491, November 2025.
ABSTRACT This interlaboratory evaluation of HepaRG CometChip was conducted to assess transferability and reproducibility of this new approach methodology (NAM) across four laboratories. Concentrations inducing up to ~70% relative cytotoxicity were determined by the organizing laboratory, and frozen chemical formulation blocks were sent to each ...
Leslie Recio, Carol Swartz, Lincoln Martin, Emily Rottinger, Stefan Pfuhler, Erica Pinkus, Daniel J. Roberts, Leon F. Stankowski, Simran Kaushal, Norah A. Owiti, Bevin P. Engelward   +10 more
wiley   +1 more source

Synergistic and Antagonistic Mutation Responses of Human MCL-5 Cells to Mixtures of Benzo[a]pyrene and 2-Amino-1-Methyl-6-Phenylimidazo[4,5-b]pyridine: Dose-Related Variation in the Joint Effects of Common Dietary Carcinogens.

, 2015
BACKGROUND: Chemical carcinogens such as benzo[a]pyrene (BaP) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) may contribute to the etiology of human diet-associated cancer.
Berenbaum MC, Glaab WE, Hawn MT, Nigel Gooderham, OECD (Organisation for Economic Co-operation and Development)., Rhiannon David, Timothy Ebbels, Zhu H   +7 more
core   +2 more sources

Origin of the TTC values for compounds that are genotoxic and/or carcinogenic and an approach for their revaluation [PDF]

, 2017
The threshold of toxicological concern (TTC) approach is a resource-effective de minimismethod for the safety assessment of chemicals, based on distributional analysis of the results of a large number of toxicological studies.
Abigail Jacobs, Alan Boobis, Altamira LLC, Chihae Yang, Corrado Lodovico Galli, Cyril Marsaux, David Kirkland, DeKeyser JG, European Commission, European Food Safety Authority (EFSA), European Food Safety Authority (EFSA), European Union Joint Research Centre (JRC), Federal Register, Federal Register, Heli Miriam Hollnagel, Jacobs MN, James Edwards, Jay Goodman, Mark Timothy David Cronin, Matthew Martin, Mirjam Luijten, Paul Brown, Rulis AM.   +22 more
core   +2 more sources

Transferability, Reproducibility and Sensitivity of Mutation Quantification by Duplex Sequencing

Environmental and Molecular Mutagenesis, Volume 66, Issue 6-7, Page 311-326, August 2025.
ABSTRACT Duplex Sequencing (DS) is an ultra‐accurate, error‐corrected next generation sequencing (ecNGS) technology for mutation analysis. A working group (WG) within Health and Environmental Sciences Institute's Genetic Toxicology Technical Committee is investigating the suitability of ecNGS for regulatory mutagenicity testing, using DS as a model ...
Shaofei Zhang, Barbara L. Parsons, Devon Fitzgerald, Anne Ashford, James Todd Auman, Tao Chen, Annette Dodge, Azeddine Elhajouji, Lena Pfaller, Shawn Harris, Jake Higgins, Cheryl A. Hobbs, Francesco Marchetti, Matthew J. Meier, Meagan B. Myers, Jesse Salk, Rebecca Sahroui, David Schuster, Raja Settivari, Stephanie L. Smith‐Roe, Carole L. Yauk, Jian Yan, Andrew Williams, Connie L. Chen   +23 more
wiley   +1 more source

Cytochrome b₅ impacts on cytochrome P450-mediated metabolism of benzo[a]pyrene and its DNA adduct formation:studies in hepatic cytochrome b₅ /P450 reductase null (HBRN) mice [PDF]

, 2018
Benzo[a]pyrene (BaP) is an environmental pollutant that, based on evidence largely from in vitro studies, exerts its genotoxic effects after metabolic activation by cytochrome P450s.
Arlt, Volker M., Barta, Frantisek, Henderson, Colin J., Indra, Radek, Kopka, Klaus, Moserova, Michaela, Mrizova, Iveta, Phillips, David H., Reed, Lindsay, Schmeiser, Heinz H., Stiborova, Marie, Wolf, C. Roland   +11 more
core   +4 more sources