Results 51 to 60 of about 541,488 (343)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Exposure Pathways of Nontuberculous Mycobacteria Through Soil, Streams, and Groundwater, Hawai'i, USA

GeoHealth, 2021
Although uncommon, nontuberculous mycobacterial (NTM) pulmonary infection in the Hawaiian Islands has a relatively high incidence and mortality compared to the mainland U.S.
Stephen T. Nelson, Schuyler Robinson, Kevin Rey, Leeza Brown, Norm Jones, Stephanie N. Dawrs, Ravleen Virdi, Grant J. Norton, L. Elaine Epperson, Nabeeh A. Hasan, Edward D. Chan, Michael Strong, Jennifer R. Honda   +12 more
doaj   +1 more source

Spatiotemporal co-existence of two Mycobacterium ulcerans clonal complexes in the Offin River Valley of Ghana [PDF]

, 2016
In recent years, comparative genome sequence analysis of African Mycobacterium ulcerans strains isolated from Buruli ulcer (BU) lesion specimen has revealed a very limited genetic diversity of closely related isolates and a striking association between ...
Aboagye, Samuel, Ampah, Kobina Assan, Asante-Poku, Adwoa, Asare, Prince, Danso, Emelia, Harris, Simon R., Kerber, Sarah, Lamelas, Araceli, Parkhill, Julian, Pluschke, Gerd, Röltgen, Katharina, Yeboah-Manu, Dorothy   +11 more
core   +2 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Assessing the accuracy of the updated Fujifilm SILVAMP TB LAM II assay between lot numbers

IJTLD Open
BACKGROUND: The Fujifilm SILVAMP TB LAM (FujiLAM) assay, a point-of-care test detecting urine-based lipoarabinomannans, has demonstrated notable diagnostic accuracy for TB. However, FujiLAM exhibits lot-to-lot variability, limiting its clinical use.
K. Chikamatsu, Y. Shimomura, A. Osugi, S. Mitarai   +3 more
doaj   +1 more source

Characterization of Mycobacterium chelonae-Like Strains by Comparative Genomics

Frontiers in Microbiology, 2017
Isolates of the Mycobacterium chelonae-M. abscessus complex are subdivided into four clusters (CHI to CHIV) in the INNO-LiPA® Mycobacterium spp DNA strip assay. A considerable phenotypic variability was observed among isolates of the CHII cluster.
Christiane L. Nogueira, Luiz G. P. de Almeida, Maria C. Menendez, Maria J. Garcia, Luciano A. Digiampietri, Erica Chimara, Margo Cnockaert, Juan C. Palomino, Françoise Portaels, Anandi Martin, Peter Vandamme, Sylvia C. Leão   +11 more
doaj   +1 more source

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

Beilstein Journal of Nanotechnology, 2020
A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines.
Gennady L. Burygin, Polina I. Abronina, Nikita M. Podvalnyy, Sergey A. Staroverov, Leonid O. Kononov, Lev A. Dykman   +5 more
doaj   +1 more source

Mycobacterium kansasii

Microbiology Spectrum, 2017
ABSTRACTThe incidence ofMycobacterium kansasiivaries widely over time and by region, but this organism remains one of the most clinically relevant isolated species of nontuberculous mycobacteria. In contrast to other common nontuberculous mycobacteria,M. kansasiiis infrequently isolated from natural water sources or soil. The major reservoir appears to
James C, Johnston, Leslie, Chiang, Kevin, Elwood   +2 more
openaire   +2 more sources

Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion

FEBS Open Bio, EarlyView.
Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng, Souvik Datta, Derrick R. Samuelson   +2 more
wiley   +1 more source

The transcriptome of Mycobacterium tuberculosis in a lipid-rich dormancy model through RNAseq analysis [PDF]

, 2017
Tuberculosis (TB) is currently the number one killer among infectious diseases worldwide. Lipids are abundant molecules during the infectious cycle of Mycobacterium tuberculosis (Mtb) and studies better mimicking its actual metabolic state during ...
Aguilar-Ayala, Diana, Deforce, Dieter, Gonzalez-Y-Merchand, Jorge A, Martin, Anandi, Palomino, Juan Carlos, Tilleman, Laurentijn, Van Nieuwerburgh, Filip, Vandamme, Peter   +7 more
core   +2 more sources