Results 141 to 150 of about 6,136 (180)

Cysteine-108 is an acylation site in myelin proteolipid protein

Biochemical and Biophysical Research Communications, 1990
Myelin proteolipid protein (PLP) contains covalently bound long-chain fatty acids. A large proportion of these acyl moieties are bound in thioester linkages, as demonstrated by alkylation of newly formed SH groups upon deacylation. To identify the Cys residue(s) involved in the thioester linkage(s), reduced and carboxyamidomethylated proteolipid ...
O A, Bizzozero, L K, Good, J E, Evans
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Electrophoretic Analysis of Myelin Proteolipid Protein and Its Deacylated Form

Journal of Neurochemistry, 1985
Abstract: Myelin proteolipid protein is known to contain covalently bound fatty acid. To determine the contribution of the fatty acid to the multiple bands observed on sodium dodecyl sulfate–polyacrylamide gel electrophoresis, the electrophoretic parameters of the proteolipid protein were compared with those of the deacylated form.
O A, Bizzozero, F, Dominguez, J M, Pasquini, E F, Soto   +3 more
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Immunochemical characterization of antibodies to the myelin proteolipid protein (PLP)

Journal of Neuroimmunology, 1988
The immunochemical specificity of antibodies raised against the bovine myelin proteolipid protein (PLP) and a series of PLP synthetic peptides was examined by enzyme-linked immunosorbent assay (ELISA) and immunoblot analyses. Polyclonal rabbit anti-bovine PLP antibodies were cross-reactive with PLP isolated from rat, monkey, and human CNS white matter,
N T, Potter, M B, Lees
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X‐ray diffraction analysis of myelin lipid/proteolipid protein multilayers

Journal of Neuroscience Research, 1989
AbstractTo examine the proposal that myelin proteolipid protein underlies the adhesion of neighboring membranes in central nervous system (CNS) myelin, we carried out X‐ray diffraction studies on the structure and interactions of model bilayers composed of total myelin lipids plus proteolipid apoprotein (PLP).
F R, Brown, J, Karthigasan, I, Singh, D A, Kirschner   +3 more
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Orientation of myelin proteolipid protein in the oligodendrocyte cell membrane

Neurochemical Research, 1996
The orientation of proteins within a cell membrane can often be difficult to determine. A number of models have been proposed for the orientation of the myelin protein, proteolipid protein (PLP), each of which includes exposed domains on the intracellular and extracellular membrane faces. Immunolabeling experiments have localized the C-terminus and the
Greer, JM, Dyer, CA, Pakaski, M, Symonowicz, C, Lees, MB   +4 more
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Myelin structure in proteolipid protein (PLP)-null mouse spinal cord

The Journal of Comparative Neurology, 1996
Fixed preparations of proteolipid protein (PLP)-null mouse spinal cord show myelin sheaths which in some regions consist of typical alternating major dense lines (MDLs) and intermediate lines (ILs) with a repeat period of 10.3 nm. More commonly, the lamellar structure consists of what appears to be a single population of dense lines, having a repeat ...
J, Rosenbluth, W, Stoffel, R, Schiff
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Structure and expression of the mouse myelin proteolipid protein gene

Journal of Neuroscience Research, 1987
AbstractThe gene for the mouse myelin proteolipid protein has been isolated and the seven exons have been sequenced. Since the sequence of a rat proteolipid protein cDNA and partial sequence of the human proteolipid protein gene have been determined, it was possible to demonstrate a very high degree of conservation for the protelipid protein gene exons
W B, Macklin, C W, Campagnoni, P L, Deininger, M V, Gardinier   +3 more
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Fatty Acid Composition of Myelin Proteolipid Protein During Vertebrate Evolution

Neurochemical Research, 1999
The hydrophobic myelin proteolipid protein (PLP) contains covalently bound long-chain fatty acids which are attached to intracellular cysteine residues via thioester linkages. To gain insight into the role of acylation in the structure and function of myelin PLP, the amount and pattern of acyl groups attached to the protein during vertebrate evolution ...
O A, Bizzozero, M B, Lees
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Molecular Biology and Neurogenetics of Myelin Proteolipid Protein

1994
Proteolipid protein (PLP) is a major component of myelin in the central nervous system where it constitutes approximately 50% of the total myelin protein by mass. This alone suggests an important structural function of PLP in compact myelin. More recently, the application of molecular genetic techniques by several groups has revealed an unexpected ...
Klaus-Armin Nave, Armin Schneider, Carol Readhead, Ian Griffiths, Anja Pühlhofer, Angelika Bartholomä, Susanna Graf, Beate Kiefer   +7 more
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Cloned Proteolipid Protein and Myelin Basic Protein cDNA. Transcription of the Two Genes During Myelination

Biological Chemistry Hoppe-Seyler, 1986
cDNA clones of rat brain proteolipid protein (PLP), also named lipophilin, the major integral myelin membrane protein, and of myelin basic protein (MBP), the major extrinsic myelin protein, have been isolated from a rat brain cDNA library cloned into the PstI site of pBR322.
M, Schaich, R M, Budzinski, W, Stoffel
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