Results 191 to 200 of about 4,942 (222)

Evolution, purification, and characterization of RC0497: a peptidoglycan amidase from the prototypical spotted fever species Rickettsia conorii

Biological chemistry, 2019
Rickettsial species have independently lost several genes owing to reductive evolution while retaining those predominantly implicated in virulence, survival, and biosynthetic pathways.
Jignesh G. Patel, H. Narra, K. Sepuru, Abha Sahni, Sandhya R. Golla, A. Sahni, Amber Singh, Casey L. C. Schroeder, I. Chowdhury, V. Popov, S. Sahni   +10 more
semanticscholar   +1 more source

Isolation and Characterization of a Weizmannia coagulans Bacteriophage Youna2 and Its Endolysin PlyYouna2

Journal of Microbiology and Biotechnology, 2023
Weizmannia coagulans (formerly Bacillus coagulans) is Gram-positive, and spore-forming bacteria causing food spoilage, especially in acidic canned food products. To control W.
Bokyung Son, Youna Kim, Booyoung Yu, M. Kong   +3 more
semanticscholar   +1 more source

Activity of N-acetylmuramoyl-L-alanine amidase in phospholipidic environments.

Biochimica et biophysica acta, 1981
A purified preparation of N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), a murein hydrolase from Escherichia coli, was found to lose its activity during incubation in the presence of bacterial phospholipid suspensions. Whether it was co-dispersed with the phospholipids or added to sonicated phospholipid suspension, the enzyme was inhibited (or ...
E, Vanderwinkel, M, de Vlieghere, L, de Tanhoffer de Volcsey   +2 more
openaire   +1 more source

Modulation of the bacterial cell wall by N‐acetylmuramoyl‐L‐alanine amidases

2016
Die Bakterienzellwand ist eine hochdynamische Struktur, die einem ständigen Wandel unterliegt, um ihre verschiedenen Aufgaben zu erfüllen. Diese reichen von physischem Schutz gegen äußere Belastungen über die Aufrechterhaltung der Zellhomöostase bis zur Immunevasion. Ein Hauptbestandteil der bakteriellen Zellwand ist das Peptidoglycan (PGN). Es ist aus
openaire   +1 more source

Biological characterization of the phage lysin AVPL and its efficiency against Aerococcus viridans-induced mastitis in a murine model

Applied and Environmental Microbiology
Aerococcus viridans (A. viridans) is an important opportunistic zoonotic pathogen that poses a potential threat to the animal husbandry industry, such as cow mastitis, due to the widespread development of multidrug-resistant strains.
Hengyu Xi, Yalu Ji, Yao Fu, Chong Chen, W. Han, J. Gu   +5 more
semanticscholar   +1 more source

Overexpression, Purification, and Characterization ofBacillus subtilis N-Acetylmuramoyl-L-alanine Amidase CwlC

Bioscience, Biotechnology, and Biochemistry, 2000
N-acetylmuramoyl-L-alanine amidase CwlC of Bacillus subtilis was overproduced in Escherichia coli and purified 21-fold. The amidase hydrolyzed type A cell walls such as B. subtilis. The amidase bound slightly to the Microbacterium lacticum cell wall (type B), but did not entirely hydrolyze it.
T, Shida, H, Hattori, F, Ise, J, Sekiguchi   +3 more
openaire   +2 more sources

A novel phage lysin Lys19 can serve as potential biocontrol agent against Clostridium perfringens and Streptococcus suis.

Journal of food microbiology
Food contamination with Clostridium perfringens (C. perfringens) and Streptococcus suis (S. suis) may lead to severe foodborne illnesses in humans, and new effective strategies are needed to limit foodborne microbial contamination. In this study, a novel
Yalu Ji, Yuqing Zhou, Yao Fu, Changjiang Sun, Wenyu Han, Xin Feng, J. Gu   +6 more
semanticscholar   +1 more source

Nature of the interactions involved in the lipid-protein complexes of the Escherichia coli N-acetylmuramoyl-L-alanine amidase.

Biochimica et biophysica acta, 1987
Depending on its concentration, phosphatidylglycerol, one of the three main Escherichia coli phospholipid species, is able to activate or inactivate the E. coli murein amidase (N-acetylmuramoyl-L-alanine amidase, EC 3.5.1.28) (Vanderwinkel, E. and De Vlieghere, M. (1985) Biochim. Biophys. Acta 838, 54-59).
E, Vanderwinkel, M, de Vlieghere, P, Charles, V, Baptist   +3 more
openaire   +1 more source

Proteomic Profiling Identifies N -Acetylmuramoyl- l -Alanine Amidase as a Novel Biomarker of Sepsis

Biomarkers in Medicine, 2016
Sepsis is a critical condition that leads to high mortality and is the most common cause of death in intensive care units. Despite exhaustive efforts by the scientific community, a reliable biomarker for diagnosis, evolution and prognosis of sepsis is still lacking.
Fabiano, Pinheiro da Silva, Thais Regiani, Cataldi, Thais Martins, de Lima, Paulo Nogueira, Starzynski, Hermes Vieira, Barbeiro, Monica Teresa Veneziano, Labate, Marcel Cerqueira Cesar, CéMachado, Heraldo Possolo, de Souza, Carlos Alberto, Labate   +8 more
openaire   +2 more sources

ประสิทธิภาพของเอนไซม์ N-Acetylmuramoyl-L-Alanine-Amidase จาก Bacillus subtilis ที่ได้จากการโคลนยีนต่อการชักนำให้เซลล์แบคทีเรียแตก

N- acetylmuramoyl-L-alanine amidase ขนาด 30 กิโลดาลตันของ Bacillus subtilis 168 ซึ่งถอดรหัสมาจากยีน cwl A ในพลาสติก Pinpoint Xa-1 มีบริเวณให้ไบโอตินมาเกาะ จึงสามารถทำให้บริสุทธิ์ขึ้นได้โดย affinity chromatography ชนิด avidin resin การทดสอบประสิทธิภาพของเอนไซม์กึ่งบริสุทธิ์ในการทำให้แบคทีเรีย 6 สายพันธุ์คือ Bacillus subtilis 168 Micrococcus luteus TISTR
openaire   +1 more source