Results 91 to 100 of about 836,707 (292)

Long-lasting cross-protection against influenza A by neuraminidase and M2e-based immunization strategies [PDF]

, 2016
There is mounting evidence that in the absence of neutralizing antibodies cross-reactive T cells provide protection against pandemic influenza viruses. Here, we compared protection and CD8+ T cell responses following challenge with H1N1 2009 pandemic and
Callewaert, Nico, Fiers, Walter, Saelens, Xavier, Schepens, Bert, Schotsaert, Michael, Smet, Anouk, Stegalkina, Svetlana, Vanderschaeghe, Dieter, Vogel, Thorsten U, Ysenbaert, Tine   +9 more
core   +2 more sources

Posttranslational modifications of heterologous proteins expressed in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
Summary The success of Nicotiana benthamiana as a workhorse for heterologous protein production is closely linked to its accessibility and tolerance to genetic manipulation, allowing efficient engineering of posttranslational protein modifications (PTMs) that are critical for the function and stability of heterologous proteins.
Kathrin Göritzer, Somanath Kallolimath, Richard Strasser   +2 more
wiley   +1 more source

The Role of the Hexosamine-Sialic Acid Metabolic Pathway Mediated by GFPT1/NANS in c-Myc-Driven Hepatocellular CarcinomaSummary

Cellular and Molecular Gastroenterology and Hepatology
Background & Aims: Hepatocellular carcinoma (HCC) frequently involves metabolic reprogramming, which promotes oncogenesis and metastasis. However, the underlying molecular mechanisms remain insufficiently explored.
Shiguan Wang, Pan Han, Ping Mi, Chunxue Wang, Miao Lu, Xinying Li, Bowen Xu, Haoran Wang, Yingchen Gao, Yanlei Hou, Xueying Tan, Jinyuan Liang, Xue Ding, Yan Zhang, Tingguo Zhang, Detian Yuan, Lei Gao, Cuijuan Zhang   +17 more
doaj   +1 more source

Preferential Accumulation of 14C-N-Glycolylneuraminic Acid over 14C-N-Acetylneuraminic Acid in the Rat Brain after Tail Vein Injection. [PDF]

PLoS ONE, 2015
The two main molecular species of sialic acid existing in nature are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). Neu5Ac is abundant in mammalian brains and plays crucial roles in many neural functions.
Risa Taguchi, Akira Minami, Yukino Matsuda, Tadanobu Takahashi, Tadamune Otsubo, Kiyoshi Ikeda, Takashi Suzuki   +6 more
doaj   +1 more source

Conservation of structure and mechanism in primary and secondary transporters exemplified by SiaP, a sialic acid binding virulence factor from Haemophilus influenzae [PDF]

, 2006
Extracytoplasmic solute receptors (ESRs) are important components of solute uptake systems in bacteria, having been studied extensively as parts of ATP binding cassette transporters.
Kelly, D.J., Muller, A., Mulligan, C., Severi, E., Thomas, G.H., Watts, A.G., Wilkinson, A.J., Wilson, K.S.   +7 more
core   +2 more sources

Tumor desialylation surpasses anti‐PD‐L1 checkpoint therapy in restoring anti‐tumor immunity in a murine model for colorectal cancer

International Journal of Cancer, Volume 157, Issue 9, Page 1948-1962, 1 November 2025.
What's new? Our research highlights how tumor sialylation affects tumor growth and reshapes the immune tumor microenvironment in colorectal cancer. We demonstrate for the first time that complete removal of sialic acids from colorectal tumor cells elicits a robust anti‐tumor immune response, but does not enhance anti‐PD‐L1 blockade efficacy.
Irene van der Haar Àvila, Tao Zhang, Victor Lorrain, Eelco Keuning, Laraib Amir Ali, Cora Chadick, Sara García‐García, Louis Boon, Noortje de Haan, Juan J. García‐Vallejo, Yvette van Kooyk, Sandra J. van Vliet   +11 more
wiley   +1 more source

Abnormalities of glycosphingolipids in mucopolysaccharidosis type III B.

Journal of Lipid Research, 1984
Glycosphingolipids from brain, liver, and spleen of a patient with mucopolysaccharidosis type III B were quantitatively analyzed. Neutral glycosphingolipids containing glucosylceramide, lactosylceramide, globotriaosylceramide, globotetraosylceramide, and
A Hara, N Kitazawa, T Taketomi
doaj   +1 more source

A Surface Plasmon Resonance‐Based Integrated Assay for Quantification and Glycosylation Characterization of Monoclonal Antibodies in Crude Heterogeneous Samples

Biotechnology and Bioengineering, Volume 122, Issue 10, Page 2724-2738, October 2025.
ABSTRACT The rise in cancer, autoimmune, inflammatory, and infectious diseases in recent decades has led to a surge in the development of monoclonal antibodies (mAbs) therapies, now the most widely used family of biologics. To meet the growing global demand, biopharmaceutical industries are intensifying their production processes.
Ilona Metayer, Catherine Forest‐Nault, Julie Guimond, Simon Joubert, Olivier Henry, Yves Durocher, Gregory De Crescenzo, Jimmy Gaudreault   +7 more
wiley   +1 more source

Reaction Mechanism of N-Acetylneuraminic Acid Lyase Revealed by a Combination of Crystallography, QM/MM Simulation, and Mutagenesis

ACS Chemical Biology, 2014
N-Acetylneuraminic acid lyase (NAL) is a Class I aldolase that catalyzes the reversible condensation of pyruvate with N-acetyl-d-mannosamine (ManNAc) to yield the sialic acid N-acetylneuraminic acid (Neu5Ac).
Adam D Daniels, I. Campeotto, M. W. van der Kamp, A. Bolt, C. Trinh, S. Phillips, A. Pearson, A. Nelson, A. Mulholland, A. Berry   +9 more
semanticscholar   +1 more source

Outer Membrane Vesicles as a Versatile Platform for Vaccine Development: Engineering Strategies, Applications and Challenges

Journal of Extracellular Vesicles, Volume 14, Issue 9, September 2025.
ABSTRACT Outer membrane vesicles (OMVs) are nanosized vesicles naturally secreted by Gram‐negative bacteria and represent a promising platform for vaccine development. OMVs possess inherent immunostimulatory properties due to the presence of pathogen‐associated molecular patterns (PAMPs), providing self‐adjuvanting capabilities and the ability to ...
Asja Garling, Frédéric Auvray, Mathieu Epardaud, Éric Oswald, Priscilla Branchu   +4 more
wiley   +1 more source