Results 1 to 10 of about 13,066,219 (329)

Characterization of recombinant human lactoferrin N-glycans expressed in the milk of transgenic cows. [PDF]

PLoS ONE, 2017
Lactoferrin (LF) is one of the most abundant bioactive glycoproteins in human milk. Glycans attached through N-glycosidic bonds may contribute to Lactoferrin functional activities. In contrast, LF is present in trace amounts in bovine milk.
Annabelle Le Parc, Sercan Karav, Camille Rouquié, Elizabeth A Maga, Apichaya Bunyatratchata, Daniela Barile   +5 more
doaj   +14 more sources

Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation

Nature Communications, 2022
Poor peptide fragmentation and unusual glycan structures limit mass spectrometry-based analysis of intact N-glycopeptides. Here, the authors develop Glyco-Decipher, a glycan-independent peptide search tool, to tackle these issues and improve the coverage
Zheng Fang, Hongqiang Qin, Jiawei Mao, Zhongyu Wang, Na Zhang, Yan Wang, Luyao Liu, Yongzhan Nie, Mingming Dong, Mingliang Ye   +9 more
doaj   +2 more sources

Glycan profiling shows unvaried N-glycomes in MSC clones with distinct differentiation potentials [PDF]

Frontiers in Cell and Developmental Biology, 2016
Different cell types have different N-glycomes in mammals. This means that cellular differentiation is accompanied by changes in the N-glycan profile. Yet when the N-glycomes of cell types with differing fates diverge is unclear. We have investigated the
Katherine Mary Wilson, Jane Elizabeth Thomas-Oates, Paul eGenever, Daniel eUngar   +3 more
doaj   +6 more sources

Uncommon N-Glycan Structures in Anhydrobiotic Tardigrades. [PDF]

Mol Cell Proteomics
We characterized the N-glycosylation profiles of anhydrobiotic tardigrades, R. varieornatus and H. exemplaris, identifying high-mannose, paucimannose, and complex-type oligosaccharides, while hybrid-type glycans were undetectable. Notably, paucimannose-type oligosaccharides accounted for 39% of the N-glycans in R. varieornatus and 17% in H. exemplaris,
Yagi H, Saito T, Guu SY, Yamakawa N, Shimamura S, Kondo S, Yagi-Utsumi M, Takai K, Furukawa JI, Guérardel Y, Khoo KH, Arakawa K, Kato K.   +12 more
europepmc   +4 more sources

Species-specific N-glycan patterns in animal and human breast milk samples [PDF]

Frontiers in Nutrition
The main components of milk are protein, fat and carbohydrates. Glycans are complex carbohydrate structures covalently bound to proteins and lipids or exist freely in solutions.
Davide Ret, Davide Ret, Salvatore Alessio Gentile, Johanna Rohrhofer, Larissa Koidl, Francesca Cozzi, Eva Untersmayr   +6 more
doaj   +2 more sources

Identification of a free-N-glycan with difucosylated type-1 lactosamine in cancerous tissue and its validation for cancer diagnosis by urinary testing [PDF]

Scientific Reports
Numerous urinary acidic free-glycan tumor marker candidates have been identified based on the glycan structure profiles of human cancer tissues. In this study, to identify novel glycan tumor markers, we performed precise glycan structural analysis ...
Miki Tanaka-Okamoto, Ken Hanzawa, Takashi Yamamoto, Kazuyoshi Ohkawa, Ken-ichi Yoshida, Satoshi Takenaka, Masayuki Ohue, Tomoyuki Yamasaki, Yasuhide Miyamoto   +8 more
doaj   +2 more sources

Recent advances in N-glycan biomarker discovery among human diseases. [PDF]

Acta Biochim Biophys Sin (Shanghai)
N-glycans play important roles in a variety of biological processes. In recent years, analytical technologies with high resolution and sensitivity have advanced exponentially, enabling analysts to investigate N-glycomic changes in different states. Specific glycan and glycosylation signatures have been identified in multiple diseases, including cancer,
Wang Y, Liu Y, Liu S, Cheng L, Liu X.
europepmc   +2 more sources

Seamless Coupling of Chemical Glycan Release and Labeling for an Accelerated Protein N-Glycan Sample Preparation Workflow

Bioengineering, 2023
Analytical methods fr direct quantitative N-glycan analysis require a sequence of sample preparation and clean-up steps that result in reduced glycan recovery. Therefore, we aimed to combine glycan release and labeling steps. Based on the hypothesis that
Mumtaz Kasim, Anja Griebel, Grit Sandig, Robert Höltzel, Akshay Malhotra, Stephan Hinderlich, Volker Sandig, Barbara Müller, Hans Henning von Horsten   +8 more
doaj   +1 more source

Production and N-glycan engineering of Varlilumab in Nicotiana benthamiana

Frontiers in Plant Science, 2023
N-glycan engineering has dramatically evolved for the development and quality control of recombinant antibodies. Fc region of IgG contains two N-glycans whose galactose terminals on Fc-glycan have been shown to increase the stability of CH2 domain and ...
Kim Dua Nguyen, Hiroyuki Kajiura, Hiroyuki Kajiura, Ryo Kamiya, Takahiro Yoshida, Ryo Misaki, Ryo Misaki, Kazuhito Fujiyama, Kazuhito Fujiyama, Kazuhito Fujiyama   +9 more
doaj   +1 more source

The Impact of Glycoengineering on the Endoplasmic Reticulum Quality Control System in Yeasts

Frontiers in Molecular Biosciences, 2022
Yeasts are widely used and established production hosts for biopharmaceuticals. Despite of tremendous advances on creating human-type N-glycosylation, N-glycosylated biopharmaceuticals manufactured with yeasts are missing on the market.
Mari A. Piirainen, Alexander D. Frey, Alexander D. Frey   +2 more
doaj   +1 more source