Results 31 to 40 of about 13,626,059 (252)

Combining functional metagenomics and glycoanalytics to identify enzymes that facilitate structural characterization of sulfated N-glycans

Microbial Cell Factories, 2021
Background Sulfate modification of N-glycans is important for several biological functions such as clearance of pituitary hormones or immunoregulation.
Léa Chuzel, Samantha L. Fossa, Madison L. Boisvert, Samanta Cajic, René Hennig, Mehul B. Ganatra, Udo Reichl, Erdmann Rapp, Christopher H. Taron   +8 more
doaj   +1 more source

Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the Virion Glycome [PDF]

, 2014
Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins.
Scrivens, JH, Crispin, Max, Huiskonen, JT, Scrivens, James H., Bitto, David, Crispin, Matthew, Harvey, David J, Bitto, D, Scrivens, James, Huiskonen, Juha T, Bowden, Thomas A, Crispin, M, Bowden, Thomas A., Scrivens, James H, Edgeworth, Matthew, Halldorsson, Steinar, Huiskonen, Juha T., Bonomelli, Camille, Edgeworth, M, Bowden, TA, Bonomelli, C, Harvey, David J., Harvey, DJ, Halldorsson, S   +23 more
core   +1 more source

Fragments of bacterial endoglycosidase S and immunoglobulin G reveal subdomains of each that contribute to deglycosylation [PDF]

, 2014
Endoglycosidase S (EndoS) is a glycoside-hydrolase secreted by the bacterium Streptococcus pyogenes. EndoS preferentially hydrolyzes the N-linked glycans from the Fc region of IgG during infection. This hydrolysis impedes Fc functionality and contributes
Yu, Xiaojie, Crispin, Max, Vesiljevic, Snezana, Pritchard, Laura K., Mattick, Susan, Scanlan, Christopher N., Baruah, Kavitha, Schnell, Jason R., Higgins, Matthew K., Krishna, Benjamin, Harvey, David J., Dixon, Emma V., Claridge, Jolyon K., Zitzmann, Nicole   +13 more
core   +1 more source

Identification and comparison of N-glycome profiles from common dietary protein sources

Food Chemistry: X
The N-glycomes of bovine whey, egg white, pea, and soy protein isolates are described here. N-glycans from four protein isolates were analyzed by HILIC high performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry (HILIC ...
Matthew Bolino, İzzet Avcı, Hacı Mehmet Kayili, Hatice Duman, Bekir Salih, Sercan Karav, Steven A. Frese   +6 more
doaj   +1 more source

Advances in understanding N-glycosylation structure, function, and regulation in health and disease

European Journal of Cell Biology, 2021
N-linked glycosylation is a post-translational modification crucial for membrane protein folding, stability and other cellular functions. Alteration of membrane protein N-glycans is implicated in wide range of pathological conditions including cancer ...
Sally Esmail, Morris F. Manolson
doaj   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

The Cerebrospinal Fluid Free-Glycans Hex₁ and HexNAc₁Hex₁Neu5Ac₁ as Potential Biomarkers of Alzheimer’s Disease

Biomolecules
Alzheimer’s disease (AD) is the most common neurodegenerative disorder, affecting a growing number of elderly people. In order to improve the early and differential diagnosis of AD, better biomarkers are needed.
Lynn Krüger, Karina Biskup, Carola G. Schipke, Bianca Kochnowsky, Luisa-Sophie Schneider, Oliver Peters, Véronique Blanchard   +6 more
doaj   +1 more source

Two-Dimensional N-Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry

Biomolecules, 2015
A new mass spectrometry imaging approach to simultaneously map the two-dimensional distribution of N-glycans in tissues has been recently developed. The method uses Matrix Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI-IMS) to ...
Thomas W. Powers, Stephanie Holst, Manfred Wuhrer, Anand S. Mehta, Richard R. Drake   +4 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Streamlined gram-scale natural N-glycan production using reversible tagging after oxidative release of natural glycans

Communications Chemistry
The study of natural glycans is fundamental to modern glycomics; however, the functional analysis of these glycans is limited by their low natural abundance, structural heterogeneity, and the absence of efficient preparative-scale isolation methods. Here,
Qing Zhang, Yi Lasanajak, Menxin Yan, Golden Chen, Xuezheng Song   +4 more
doaj   +1 more source