Results 11 to 20 of about 923,736 (263)
“NAD-capQ” detection and quantitation of NAD caps [PDF]
RNA, 2018 RNA 5′ cap structures comprising the metabolic effector nicotinamide adenine dinucleotide (NAD) have been identified in diverse organisms. Here we report a simple, two-step procedure to detect and quantitate NAD-capped RNA, termed “NAD-capQ.” By use of NAD-capQ we quantitate NAD-capped RNA levels in Escherichia coli, Saccharomyces cerevisiae, and human
Jeremy G. Bird +3 more
openaire +7 more sources
Nicotinamide adenine dinucleotide (NAD+). Formal potential of the NAD+/NAD· couple and NAD· dimerization rate [PDF]
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1984 The rate constant, kd, for the dimerization of the free radical (NAD), produced on the initial one-electron reduction of NAD+, was measured by double potential-step chronoamperometry, fast-scan cyclic voltammetry (cathodic-anodic peak current ratio) and slow-scan cyclic voltammetry (peak potential shift) for a medium in which neither NAD+ nor its ...
Mark A. Jensen, Philip J. Elving
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NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana
STAR Protocols, 2021 Eukaryotic RNAs can be modified with a non-canonical 5' nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD ...
Xiang Yu +2 more
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Acta Crystallographica Section F, Volume 78, Issue 2, Page 52-58, February 2022., 2022 The crystal structure of a short‐chain dehydrogenase/reductase from Burkholderia phymatum offers insights into its possible functions and likely inhibitors of its enzymatic functions.Burkholderia phymatum is an important symbiotic nitrogen‐fixing betaproteobacterium. B.
Jawaher Alenazi +4 more
wiley +1 more source
Historical Agricultural Landforms—Central European Bio-Cultural Heritage Worthy of Attention
Land, 2022 Knowledge about past agricultural land management can bring solutions for future needs. One undervalued historical type of historical rural landscape in temperate Europe is termed plužiny.
Johana Zacharová +9 more
doaj +1 more source
The FEBS Journal, Volume 289, Issue 4, Page 955-964, February 2022., 2022 Increased IDO activity towards de novo nicotinamide synthesis is a hallmark of alternatively activated macrophages. IDO upregulation in alternatively activated macrophages may be an attempt to compensate for insufficient NAD availability. Manipulation of nicotinamide metabolism may shift macrophage metabolism, thereby providing a therapeutic approach ...
Melinda S. Suchard, Dana M. Savulescu
wiley +1 more source
Assays for NAD+-Dependent Reactions and NAD+ Metabolites [PDF]
, 2018 Nicotinamide adenine dinucleotide (NAD+) is an essential redox cofactor and signaling molecule that controls the activity of enzymes involved in metabolism, DNA repair, and cellular survival, such as the PARPs, CD38, and the sirtuins. Here, we describe three methods for measuring the activity of these enzymes: the etheno-NAD+ assay measures NAD ...
David A. Sinclair +4 more
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Grabbing the genome by the NADs [PDF]
Chromosoma, 2015 The regions of the genome that interact frequently with the nucleolus have been termed nucleolar-associated domains (NADs). Deep sequencing and DNA-fluorescence in situ hybridization (FISH) experiments have revealed that these domains are enriched for repetitive elements, regions of the inactive X chromosome (Xi), and several RNA polymerase III ...
Matheson, Timothy D., Kaufman, Paul D.
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Acta Crystallographica Section D, Volume 77, Issue 10, Page 1317-1335, October 2021., 2021 The homospermidine synthases from P. aeruginosa and B. viridis, as well as their single‐residue variants, are compared based on crystal structures and activity assays. A high structural similarity is demonstrated, suggesting the equivalent involvement of relevant residues in the reaction mechanism and catalytic dependence on cation–π interaction ...
F. Helfrich, Axel J. Scheidig
wiley +1 more source
Development of the Cerebrospinal Fluid in Early Stage after Hemorrhage in the Central Nervous System
Life, 2021 Extravasation of blood in the central nervous system (CNS) represents a very strong damaged associated molecular patterns (DAMP) which is followed by rapid inflammation and can participate in worse outcome of patients.
Petr Kelbich +10 more
doaj +1 more source