Results 1 to 10 of about 158,023 (302)

Homeostatic regulation of NAD(H) and NADP(H) in cells [PDF]

Genes and Diseases
Nicotinamide adenine dinucleotide (NAD+)/reduced NAD+ (NADH) and nicotinamide adenine dinucleotide phosphate (NADP+)/reduced NADP+ (NADPH) are essential metabolites involved in multiple metabolic pathways and cellular processes.
Luojun Chen, Xiaoke Xing, Pingfeng Zhang, Lulu Chen, Huadong Pei   +4 more
doaj   +4 more sources

Cytoplasmic NAD/H synthesis via NRK1 regulates inflammatory capacity and promotes survival of CD4+ T cells [PDF]

Nature Communications
T cell metabolism increases upon activation, underpinning immune effector functions. Nicotinamide adenine dinucleotide (NAD/H) is an essential redox cofactor for glycolysis and mitochondrial substrate oxidation.
Victoria Stavrou, Myah Ali, Nancy Gudgeon, Emma L. Bishop, Taylor Fulton-Ward, Bethany Turley, Silke Heising, Sally H. Mohamed, Sofia Hain, Lorna George, Minghao Deng, Jack McCowan, Lozan Sheriff, Scott P. Davies, Bryan Marzullo, Daniel A. Tennant, Craig L. Doig, David A. Bending, Ed W. Roberts, Gareth G. Lavery, Rebecca A. Drummond, Sarah Dimeloe   +21 more
doaj   +2 more sources

Visualizing subcellular changes in the NAD(H) pool size versus redox state using fluorescence lifetime imaging microscopy of NADH [PDF]

Communications Biology
NADH autofluorescence imaging is a promising approach for visualizing energy metabolism at the single-cell level. However, it is sensitive to the redox ratio and the total NAD(H) amount, which can change independently from each other, for example with ...
Angela Song, Nicole Zhao, Diana C. Hilpert, Caroline Perry, Joseph A. Baur, Douglas C. Wallace, Patrick M. Schaefer   +6 more
doaj   +2 more sources

Streptococcus pyogenes can support or inhibit growth of Haemophilus influenzae by supplying or restricting extracellular NAD.

PLoS ONE, 2022
Nicotinamide adenine dinucleotide (NAD+) is an essential co-factor for cellular metabolism and serves as a substrate in enzymatic processes. NAD+ is produced by de novo synthesis or salvage pathways in nearly all bacterial species. Haemophilus influenzae
Hyunju Lee, Rebecca J Edgar, Ian J Lichtenstein, Jorge J Velarde, Natalia Korotkova, Michael R Wessels   +5 more
doaj   +2 more sources

Meta-analysis of NAD(P)(H) quantification results exhibits variability across mammalian tissues

Scientific Reports, 2023
Nicotinamide Adenine Dinucleotide (NAD+) plays an important role in energy metabolism and signaling pathways controlling crucial cellular functions. The increased interest in NAD+ metabolism and NAD+-boosting therapies has reinforced the necessity for ...
Dassine Azouaoui, Michael René Choinière, Momtafin Khan, Shahab Sayfi, Simran Jaffer, Selvia Yousef, David A. Patten, Alexander E. Green, Keir J. Menzies   +8 more
doaj   +1 more source

NAD⁺-carrying mesoporous silica nanoparticles can prevent oxidative stress-induced energy failures of both rodent astrocytes and PC12 cells. [PDF]

PLoS ONE, 2013
AIM: To test the hypothesis that NAD(+)-carrying mesoporous silica nanoparticles (M-MSNs@NAD+) can effectively deliver NAD(+) into cells to produce cytoprotective effects. METHODS & MATERIALS: NAD(+) was incorporated into M-MSNs.
Heyu Chen, Yao Wang, Jixi Zhang, Yingxin Ma, Caixia Wang, Ying Zhou, Hongchen Gu, Weihai Ying   +7 more
doaj   +1 more source

Fluorescence lifetime imaging unravels C. trachomatis metabolism and its crosstalk with the host cell. [PDF]

PLoS Pathogens, 2011
Chlamydia trachomatis is an obligate intracellular bacterium that alternates between two metabolically different developmental forms. We performed fluorescence lifetime imaging (FLIM) of the metabolic coenzymes, reduced nicotinamide adenine dinucleotides
Márta Szaszák, Philipp Steven, Kensuke Shima, Regina Orzekowsky-Schröder, Gereon Hüttmann, Inke R König, Werner Solbach, Jan Rupp   +7 more
doaj   +1 more source

Monitoring microenvironment of Hep G2 cell apoptosis using two-photon fluorescence lifetime imaging microscopy

Journal of Innovative Optical Health Sciences, 2022
Apoptosis is very important for the maintenance of cellular homeostasis and is closely related to the occurrence and treatment of many diseases. Mitochondria in cells play a crucial role in programmed cell death and redox processes.
Kexin Wang, Shiyao Tang, Shiqi Wang, Fangrui Lin, Gengjin Zou, Junle Qu, Liwei Liu   +6 more
doaj   +1 more source

Label-free characterization of single extracellular vesicles using two-photon fluorescence lifetime imaging microscopy of NAD(P)H

Scientific Reports, 2021
The heterogeneous nature of extracellular vesicles (EVs) creates the need for single EV characterization techniques. However, many common biochemical and functional EV analysis techniques lack single EV resolution.
Janet E. Sorrells, Elisabeth M. Martin, Edita Aksamitiene, Prabuddha Mukherjee, Aneesh Alex, Eric J. Chaney, Marina Marjanovic, Stephen A. Boppart   +7 more
doaj   +1 more source

CHARACTERIZING FLUORESCENCE LIFETIME OF NAD(P)H IN HUMAN LEUKEMIC MYELOID CELLS AND MONONUCLEAR CELLS [PDF]

Journal of Innovative Optical Health Sciences, 2013
The aim of this ex vivo study was to explore the potential of using the fluorescence lifetime of intracellular reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) as a label-free indicator to characterize the differences between human ...
LI-SHENG LIN, LI-NA LIU, HUI-FANG HUANG, YUAN-ZHONG CHEN, BU-HONG LI, ZHENG HUANG   +5 more
doaj   +1 more source