Diphenyleneiodonium, an inhibitor of NOXes and DUOXes, is also an iodide-specific transporter
NADPH oxidases (NOXes) and dual oxidases (DUOXes) generate O2.− and H2O2. Diphenyleneiodonium (DPI) inhibits the activity of these enzymes and is often used as a specific inhibitor.
C. Massart+5 more
doaj +1 more source
Monitoring of the Membrane Potential in Proteoliposomes with Incorporated Cytochrome-c Oxidase Using the Fluorescent Dye Indocyanine [PDF]
A method has been developed to monitor changes of the membrane potential across vesicle membranes in real time. Using the potential-sensitive fluorescent dye indocyanine and on the basis of water/lipid redistribution model, a calculation procedure has been introduced to estimate the membrane potential in vesicles with incorporated cytochrome-c oxidase.
arxiv
Electron and proton transport by NADPH oxidases [PDF]
The NADPH oxidase is the main weapon of phagocytic white blood cells that are the first line of defence of our body against invading pathogens, and patients lacking a functional oxidase suffer from severe and recurrent infections. The oxidase is a multisubunit enzyme complex that transports electrons from cytoplasmic NADPH to molecular oxygen in order ...
Demaurex, Nicolas, Petheö, Gábor L
openaire +4 more sources
Glucose-6-phosphate dehydrogenase (G6PDH or G6PD) is the key regulatory enzyme in the oxidative pentose phosphate pathway (OPPP). The cytosolic isoforms including G6PD5 and G6PD6 account for the major part of the G6PD total activity in plant cells. Here,
Lei Yang+9 more
doaj +1 more source
Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy [PDF]
Cytochrome C oxidase and FoF1-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enzymes are located in the inner mitochondrial membrane (IMM), which exhibits large invaginations called cristae.
arxiv
NADPH Oxidases in Cardiovascular Health and Disease [PDF]
Increased oxidative stress plays an important role in the pathophysiology of cardiovascular diseases such as hypertension, atherosclerosis, diabetes, cardiac hypertrophy, heart failure, and ischemia-reperfusion. Although several sources of reactive oxygen species (ROS) may be involved, a family of NADPH oxidases appears to be especially important for ...
Cave, A C+6 more
openaire +4 more sources
Surface modified sulfur nanoparticles can escape the glutathione reductase mediated detoxification system in fungi [PDF]
The antifungal effects of orthorhombic (~10 nm; spherical) and monoclinic (~50 nm; tetrapod) sulfur nanoparticles (SNPs) were studied against the NADPH-dependent glutathione reductase (GR) mediated xenobiotic detoxification system (GSH-GSSG) in filamentous fungi (Aspergillus niger as a model organism).
arxiv
Using Label-Free Raman Spectroscopy Integrated with Microfluidic Chips to Probe Ferroptosis Networks in Cells [PDF]
Ferroptosis, a regulated form of cell death driven by oxidative stress and lipid peroxidation, has emerged as a pivotal research focus with implications across various cellular contexts. In this study, we employed a multifaceted approach, integrating label-free Raman spectroscopy and microfluidics to study the mechanisms underpinning ferroptosis.
arxiv +1 more source
Elevated CO2 (eCO2) often reduces leaf stomatal aperture and density thus impacts plant physiology and productivity. We have previously demonstrated that the Arabidopsis BIG protein distinguishes between the processes of eCO2-induced stomatal closure and
Jingjing He+7 more
doaj +1 more source
Diverse Fluorescent Probe Concepts for Detection and Monitoring of Reactive Oxygen Species
Cellular ROS at physiological levels are essential for signaling and homeostasis. However, excessive ROS leads to cell death and diseases. Fluorescence technology helps in detecting and imaging ROS. This review summarizes various organic dye‐based fluorescent probe concepts and modifications to traditional dyes using dye chemistry and photophysics ...
Vineeta Chaturvedi+3 more
wiley +1 more source