Results 121 to 130 of about 14,448 (252)

Integrative Modeling in the Age of Machine Learning: A Summary of HADDOCK Strategies in CAPRI Rounds 47–55

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The HADDOCK team participated in CAPRI rounds 47–55 as server, manual predictor, and scorers. Throughout these CAPRI rounds, we used a plethora of computational strategies to predict the structure of protein complexes. Of the 10 targets comprising 24 interfaces, we achieved acceptable or better models for 3 targets in the human category and 1 ...
Victor Reys, Marco Giulini, Vlad Cojocaru, Anna Engel, Xiaotong Xu, Jorge Roel‐Touris, Cunliang Geng, Francesco Ambrosetti, Brian Jiménez‐García, Zuzana Jandova, Panagiotis I. Koukos, Charlotte van Noort, João M. C. Teixeira, Siri C. van Keulen, Manon Réau, Rodrigo V. Honorato, Alexandre M. J. J. Bonvin   +16 more
wiley   +1 more source

EXPRESSION AND PURIFICATION OF ENGINEERED ANTI SERPIN B3 (SCCA-1) NANOBODIES IN PICHIA PASTORIS: TOWARD IMPROVED CANCER DIAGNOSTICS TOOLS

reservedNanobodies sono piccoli frammenti di anticorpi derivati da camelidi, caratterizzati da dimensioni ridotte, alta stabilità, struttura semplice e capacità di legarsi con elevata specificità ai propri bersagli molecolari, proprietà che ...
ZARDINI, LAURA
core  

Functionalized nanobodies for cancer therapy

, 2014
Cancer treatment is complicated by the high similarity between cancerous and healthy tissue. New anti-cancer drugs, the monoclonal antibodies, act on one specific molecule/process and thereby minimize side effects.
van Vught, R.W.M.
core   +1 more source

Implementation of a Novel Nanobody Panel for the Efficient Capture of Extracellular Vesicles from Human Plasma

Molecules
Extracellular vesicles (EVs) are nanoscale particles released by cells and are significant components in intercellular communication. Their ability to reflect the molecular state of parental cells and their presence in body fluids make them increasingly ...
Marija Tursunović, Lidija Filipović, Ninoslav Mitić, Sanja Stevanović, Milica Spasojević Savković, Ario de Marco, Milica Popović   +6 more
doaj   +1 more source

AlphaFold and Docking Approaches for Antibody–Antigen and Other Targets: Insights From CAPRI Rounds 47–55

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Accurate modeling of the structures of protein–protein complexes and other biomolecular interactions represents a longstanding and important challenge for computational biology. The Critical Assessment of PRedicted Interactions (CAPRI) experiment has served for over two decades as a key means to assess and compare current approaches and ...
Ragul Gowthaman, Minjae Park, Rui Yin, Johnathan D. Guest, Brian G. Pierce   +4 more
wiley   +1 more source

Improving the production and stability of nanobodies

Protein Engineering, Design and Selection
Abstract Nanobodies offer unique advantages in biomedical and biotechnological applications due to their smaller size, ability to bind challenging epitopes, and affordable production using recombinant technology. However, challenges in large-scale production, stability, and solubility limit their widespread use.
Alba Pejenaute, Laura Gálvez-Larrosa, Cristina M Segovia, Pablo Herrero-Alfonso, David Fernández-Ramos, Fernando Lopitz-Otsoa, Óscar Millet, Francesca Peccati, Gonzalo Jiménez-Osés, Gabriel Ortega-Quintanilla   +9 more
openaire   +2 more sources

Massive Sampling Strategy for Antibody–Antigen Targets in CAPRI Round 55 With MassiveFold

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Massive sampling with AlphaFold2 improves protein–protein complex predictions. This has been shown during the last CASP15‐CAPRI blind prediction round by the AFsample tool. However, more difficult targets including antibody–antigen binding remain challenging. CAPRI Round 55 consisted of three antibody–antigen targets and one heterotrimer.
Nessim Raouraoua, Marc F. Lensink, Guillaume Brysbaert   +2 more
wiley   +1 more source

Biomolecular Interaction Prediction in the Pre‐ and Post‐AlphaFold Era: The 8th CAPRI Evaluation

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT We report on the 8th CAPRI Evaluation period, capturing the assessment of CAPRI Rounds 47 to 55 (excluding the CASP and COVID‐related Rounds), which have witnessed the transition to AI‐driven prediction tools such as AlphaFold and related alternatives. The prediction Rounds in this evaluation are characterized by a high level of difficulty due
Marc F. Lensink, Nessim Raouraoua, Guillaume Brysbaert, Sameer Velankar, Shoshana J. Wodak, Alexandre M. J. J. Bonvin   +5 more
wiley   +1 more source

Rational design of Nanobodies for binding and inhibition of LexA in E.coli SOS response.

reservedLa risposta SOS è un meccanismo di difesa batterico conservato e sviluppato per contrastare gli effetti da stress genotossico, come nel caso degli antibiotici ai quali sono esposti i batteri. A livello molecolare il meccanismo vede coinvolte due
MEZZETTI, RICCARDO
core  

Research Note: Clostridium perfringens NetB and CnaA neutralizing nanobodies in feed reduce the incidence of poultry necrotic enteritis

Poultry Science
: Necrotic enteritis is a devastating disease to poultry caused by the bacterium Clostridium perfringens. As a novel approach to combating poultry necrotic enteritis, we identified and characterized several hundred single domain antibody fragments (or ...
Slade A. Loutet, Sylvia Cheung, Sarah Zaytsoff, Charles Hofacre, Matthew K. Jones, Filip Van Petegem, Hamlet Abnousi   +6 more
doaj   +1 more source