Results 111 to 120 of about 14,448 (252)

Engineering Bacteria for Medicine: Delivery, Diagnosis, and Therapy

iMetaMed, EarlyView.
ABSTRACT With rapid advances in synthetic biology and genetic engineering, genetically engineered bacteria (GEB) have emerged as a promising platform for biological therapy, addressing key limitations of conventional drug delivery systems and demonstrating significant clinical potential.
Shiyu Xia, Guiling Fu, Xiaoyue Ding, Wen Yang, Ruijie Bai, Yawei Cai, Jing xuan Zhang, Yanxia Wei, Conghui Han, Yi‐Zhou Gao, Ying Li, Zuobin Zhu   +11 more
wiley   +1 more source

The T‐Cell Response Mechanism in Human Papillomavirus‐Associated Cervical Cancer and New Strategies for Immunotherapy

iNew Medicine, EarlyView.
ABSTRACT Human papillomavirus (HPV) is a double‐stranded DNA virus that infects human skin and mucosal tissues exclusively. The German scientist Harald zur Hausen was awarded the 2008 Nobel Prize in Physiology or Medicine for his discovery of the link between HPV infection and cervical cancer.
Fang Zhu, Farah Nazir, Ying Zhou, Yuhua Shang, Hongliang He, Tengchuan Jin   +5 more
wiley   +1 more source

Serial Llama Immunization with Various SARS-CoV-2 RBD Variants Induces Broad Spectrum Virus-Neutralizing Nanobodies

Vaccines
The emergence of SARS-CoV-2 mutant variants has posed a significant challenge to both the prevention and treatment of COVID-19 with anti-coronaviral neutralizing antibodies. The latest viral variants demonstrate pronounced resistance to the vast majority
Pavel P. Solodkov, Alexander M. Najakshin, Nikolai A. Chikaev, Sergey V. Kulemzin, Ludmila V. Mechetina, Konstantin O. Baranov, Sergey V. Guselnikov, Andrey A. Gorchakov, Tatyana N. Belovezhets, Anton N. Chikaev, Olga Y. Volkova, Alexander G. Markhaev, Yulia V. Kononova, Alexander Y. Alekseev, Marina A. Gulyaeva, Alexander M. Shestopalov, Alexander V. Taranin   +16 more
doaj   +1 more source

Multi‐omics biomarkers for intestinal infection and inflammation in inflammatory bowel disease: Current evidence, translational challenges, and diagnostic opportunities

Interdisciplinary Medicine, EarlyView.
Prospective multi‐site cohorts, multi‐omics profiling, and computational analysis may help identify biomarker patterns across clinical settings in IBD and superimposed infections. With further mechanistic and clinical validation, these signals could support the development of practical multi‐analyte tools for more precise diagnosis and management ...
Ziyu Yang, Jiayi Yao, Xinxian Zhao, Yan Xu, Yifan Chen, Tianchi Chen, Ying Jian, Min Li   +7 more
wiley   +1 more source

Targeting the intratumoral microbiota to overcome cancer immunotherapy resistance

Interdisciplinary Medicine, EarlyView.
This review delineates the mechanisms of intratumoral microbiota in cancer immunotherapy resistance, and offers strategies for microbiota‐targeted precision immunotherapy. Abstract Cancer remains a significant global health threat. The tumor microenvironment (TME) is a sophisticated ecological niche that exerts a pivotal effect on treatment outcomes ...
Bufu Tang, Tong Jiang, Yaling Lin, Wei Zhang, Hongjie Fan, Minjie Yang, Bingzhe Li, Yaozu Liu, Changyu Li, Xuran Jin, Wen Zhang, Zhiping Yan, Qianqian Zhao, Xuefeng Kan, Xudong Qu   +14 more
wiley   +1 more source

Binding of two nanobodies simultaneously.

(A) Analytical SEC of Arc-CTdt with nanobodies C11 and H11 indicates simultaneous binding. (B) SDS-PAGE of purified ternary complex rArcFL-7A+E5+C11. Arc is shown with the thick arrow and the two nanobodies with thin arrows.
Aleksi Sutinen (10039145), Oda C. Krokengen (6812267), Sarah Weber (153709), Andrea J. Lopez (4990781), José M. Godoy Muñoz (18462075), Lasse Neset (18215919), Sigurbjörn Markússon (10803121), Eleni Christakou (11731539), Clive R. Bramham (10181909), Petri Kursula (90066)   +9 more
core   +1 more source

Ion Activation Methods for Top‐Down Proteomics

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) has emerged as a premier method used to characterize the sequences of proteins. Top‐down proteomics aims to capture the multiple sources of structural diversity reflected in proteins, such as those that arise from alternative RNA splicing events or the addition of post‐translational modifications. Tandem MS (i.e., MS/MS)
Jada N. Walker, Jennifer S. Brodbelt
wiley   +1 more source

Targeting mGlyR with nanobodies for depression

Nature Communications
Abstract Development of therapies for neuropsychiatric conditions is one of the greatest challenges of modern medicine. Common limitations of traditional small molecule drugs include poor efficacy, off-target side effects and difficult druggability of many targets.
Laboute, Thibaut, Zucca, Stefano, Sial, Omar, Sharma, Mansi, Brunori, Gloria, Singh, Shikha, Nageswar, Kv, Peng, Haiyong, Rader, Christoph, Becker, Jérôme Aj, Le Merrer, Julie, Singh, Appu, Martemyanov, Kirill   +12 more
openaire   +3 more sources

Characterization of Nanobodies Targeting RecA: A Strategy for Bacterial SOS Response Inhibition

reservedThe global rise of antimicrobial resistance (AMR) presents a major threat to public health. A key contributor to AMR is the bacterial SOS response, a conserved DNA repair mechanism triggered by genotoxic stress.
BIRRO, GIACOMO
core  

Development of nanobodies against the coat protein of maize chlorotic mottle virus

FEBS Open Bio
Maize lethal necrosis (MLN) is a maize disease caused by the maize chlorotic mottle virus (MCMV), a potyvirus which causes yield losses of 30–100%. The present study aimed to isolate nanobodies against the MCMV coat protein (CP) for the diagnosis of MLN.
Faith Njeru, Olivier Zwaenepoel, Geert Haesaert, Gerald Misinzo, Kris De Jonghe, Jan Gettemans   +5 more
doaj   +1 more source