Results 91 to 100 of about 14,448 (252)

Nanomaterials for Allergy Diagnosis and Treatment: Advances, Opportunities and Translational Challenges

Advanced Science, EarlyView.
Nanomaterials offer dual applications in allergy management. For diagnosis, nanomaterials enhance analytical sensitivity and improve detection in specific IgE and functional assays such as the basophil activation test (BAT). For allergen‐specific immunotherapy, nanomaterials enable allergen masking and controlled release, and effectively modulate the ...
Madiha Habib, Shan Jiang, Joyce ZX Lee, CW Lim, HL Yeung, Nicki YH Leung, Patrick SC Leung, Ting F Leung, Christine YY Wai   +8 more
wiley   +1 more source

Anti-IgD nanobodies as novel tools for studying human IgD biology

Scientific Reports
IgD is the least well characterized of mammalian antibody isotypes and its biology remains poorly understood. Nanobodies are a useful and versatile tool for research and diagnostics, including for protein purification, capture and detection applications.
Susan K. Vester, Rebecca L. Beavil, Alexander Alexandrovich, Hannah J. Gould, Andrew J. Beavil, Brian J. Sutton, James M. McDonnell   +6 more
doaj   +1 more source

Pericentrosomal Redistribution of the Endoplasmic Reticulum Ensures Organelle Symmetric Inheritance and Mitotic Progression

Advanced Science, EarlyView.
Upon mitotic entry, RTN4 relocalizes to the pericentrosomal region, forming a more tubular ER network around centrosomes. CDK1‐mediated phosphorylation of RTN4 increases its interaction with Rab11 GTPase, facilitating dynein‐dependent transport of RTN4 to the pericentrosomal region.
Xiangyu Xu, Yalin Liu, Rongyi Wang, Wenwen Xu, Hao Shi, Ning Huang, Junlin Teng, Jin Meng, Pengli Zheng, Jianguo Chen   +9 more
wiley   +1 more source

A guide to: generation and design of nanobodies [PDF]

The FEBS Journal, 2020
A nanobody (Nb) is a registered trademark of Ablynx, referring to the single antigen‐binding domain of heavy chain‐only antibodies (HCAbs) that are circulating in Camelidae. Nbs are produced recombinantly in micro‐organisms and employed as research tools or for diagnostic and therapeutic applications.
openaire   +2 more sources

Investigating the In Vivo Effects of Anti-Prion Protein Nanobodies on Prion Disease with AAV Vector

Pathogens
Prion diseases are fatal neurodegenerative disorders affecting humans and animals, and the central pathogenic event is the conversion of normal prion protein (PrPC) into the pathogenic PrPSc isoform.
Jingjing Zhang, Mengfei Wang, Dan Wang, Xiangyi Zhang, Yue Ma, Els Pardon, Jan Steyaert, Romany Abskharon, Fei Wang, Jiyan Ma   +9 more
doaj   +1 more source

A new strategy to generate nanobodies for the coumaphos based on the synthesized nanobody libraries

Food Chemistry
To break through the bottleneck in preparation of nanobody (Nb) for chemical contaminants induced by the difficulties in the synthesis of immunogen, complexity and unexpectable efficiency of immunization, a novel strategy to generate Nbs based on the designed synthetic Nb libraries with final size up to 109 cfu/mL was adopted and succeeded in selection
Fang, Ruyu, Li, Yingxue, Liu, Fei, Liang, Yifan, Wang, Yu, Zhong, Guohua, Xu, Zhenlin, Hammock, Bruce D., Wang, Hong   +8 more
openaire   +3 more sources

CXCR4-targeting nanobodies differentially inhibit CXCR4 function and HIV entry [PDF]

, 2018
The chemokine receptor CXCR4 and its ligand CXCL12 contribute to a variety of human diseases, such as cancer. CXCR4 is also a major co-receptor facilitating HIV entry. Accordingly, CXCR4 is considered as an attractive therapeutic target.
Zhao, Chunxia, Smit, Martine J., de Haard, Hans, Smit, Martine J.; id_orcid, Van Hout, Anneleen, van der Woning, Bas, Rucker, Joseph B., Klarenbeek, Alex, Heukers, Raimond, Verrips, Theo, Vermeire, Kurt, Handel, Tracy, Van Loy, Tom, Schols, Dominique, Bobkov, Vladimir, Arimont, Marta, Doijen, Jordi, Rimkunas, Rebecca, de Graaf, Chris   +18 more
core   +1 more source

Therapeutische Nanobodies gegen SARS-CoV-2

, 2022
Monoclonal immunoglobulins are widely successful as therapeutics and have also been effective in treating COVID-19. However, their production in mammalian cells is expensive and cannot be scaled to meet the demand in a global pandemic.
Dobbelstein, M., Görlich, Dirk, Güttler, Thomas, Görlich, D., Dobbelstein, Matthias, Güttler, T.   +5 more
core   +1 more source

Development of SNAP-Tag Based Nanobodies as Secondary Antibody Mimics for Indirect Immunofluorescence Assays

Cells
The immunofluorescence assay is widely used for cellular biology and diagnosis applications. Such an antigen–antibody detection system enables the assessment and visualization of the expression and localization of target proteins.
Wenjie Sheng, Chaoyu Zhang, T. M. Mohiuddin, Marwah Al-Rawe, Roland Schmitz, Marcus Niebert, Lutz Konrad, Steffen Wagner, Felix Zeppernick, Ivo Meinhold-Heerlein, Ahmad Fawzi Hussain   +10 more
doaj   +1 more source

Fluorescent Diarylethenes With Polar Groups: Synthesis, Spectra, and Optical Microscopy Applications

Chemistry – A European Journal, EarlyView.
Blinking green and flashing red: photo‐switchable and photo‐activatable probes emitting green and red light were prepared and applied as bioconjugates in super‐resolution optical microscopy. ABSTRACT The use of photoactivatable fluorescent diarylethenes (fDAEs) in biology‐related light microscopy has been restricted by the lack of probes having freely ...
Kakishi Uno, Sven Nagorny, Ayse Aktalay, Dojin Kim, Mariano L. Bossi, Vladimir N. Belov, Stefan W. Hell   +6 more
wiley   +1 more source