Results 81 to 90 of about 16,217 (195)

EXPRESSION AND PURIFICATION OF ENGINEERED ANTI SERPIN B3 (SCCA-1) NANOBODIES IN PICHIA PASTORIS: TOWARD IMPROVED CANCER DIAGNOSTICS TOOLS

reservedNanobodies sono piccoli frammenti di anticorpi derivati da camelidi, caratterizzati da dimensioni ridotte, alta stabilità, struttura semplice e capacità di legarsi con elevata specificità ai propri bersagli molecolari, proprietà che ...
ZARDINI, LAURA
core  

Binding of two nanobodies simultaneously.

(A) Analytical SEC of Arc-CTdt with nanobodies C11 and H11 indicates simultaneous binding. (B) SDS-PAGE of purified ternary complex rArcFL-7A+E5+C11. Arc is shown with the thick arrow and the two nanobodies with thin arrows.
Aleksi Sutinen (10039145), Oda C. Krokengen (6812267), Sarah Weber (153709), Andrea J. Lopez (4990781), José M. Godoy Muñoz (18462075), Lasse Neset (18215919), Sigurbjörn Markússon (10803121), Eleni Christakou (11731539), Clive R. Bramham (10181909), Petri Kursula (90066)   +9 more
core   +1 more source

Development of nanobodies against the coat protein of maize chlorotic mottle virus

FEBS Open Bio
Maize lethal necrosis (MLN) is a maize disease caused by the maize chlorotic mottle virus (MCMV), a potyvirus which causes yield losses of 30–100%. The present study aimed to isolate nanobodies against the MCMV coat protein (CP) for the diagnosis of MLN.
Faith Njeru, Olivier Zwaenepoel, Geert Haesaert, Gerald Misinzo, Kris De Jonghe, Jan Gettemans   +5 more
doaj   +1 more source

Rational design of Nanobodies for binding and inhibition of LexA in E.coli SOS response.

reservedLa risposta SOS è un meccanismo di difesa batterico conservato e sviluppato per contrastare gli effetti da stress genotossico, come nel caso degli antibiotici ai quali sono esposti i batteri. A livello molecolare il meccanismo vede coinvolte due
MEZZETTI, RICCARDO
core  

Modular transient nanoclustering of activated β2-adrenergic receptors revealed by single-molecule tracking of conformation-specific nanobodies

, 2020
None of the current superresolution microscopy techniques can reliably image the changes in endogenous protein nanoclustering dynamics associated with specific conformations in live cells.
Rae, James, Gormal, Rachel S, Budnar, Srikanth, Padmanabhan, Pranesh, Coakley, Sean, Thomas, Walter G, Blum, Ailisa, Kasula, Ravikiran, Joensuu, Merja, Lo, Harriet P, Ferguson, Charles, Bademosi, Adekunle T, Bastiani, Michele, Giacomotto, Jean, Wallis, Tristan P, et al.   +15 more
core   +1 more source

Implementation of a Novel Nanobody Panel for the Efficient Capture of Extracellular Vesicles from Human Plasma

Molecules
Extracellular vesicles (EVs) are nanoscale particles released by cells and are significant components in intercellular communication. Their ability to reflect the molecular state of parental cells and their presence in body fluids make them increasingly ...
Marija Tursunović, Lidija Filipović, Ninoslav Mitić, Sanja Stevanović, Milica Spasojević Savković, Ario de Marco, Milica Popović   +6 more
doaj   +1 more source

Research Note: Clostridium perfringens NetB and CnaA neutralizing nanobodies in feed reduce the incidence of poultry necrotic enteritis

Poultry Science
: Necrotic enteritis is a devastating disease to poultry caused by the bacterium Clostridium perfringens. As a novel approach to combating poultry necrotic enteritis, we identified and characterized several hundred single domain antibody fragments (or ...
Slade A. Loutet, Sylvia Cheung, Sarah Zaytsoff, Charles Hofacre, Matthew K. Jones, Filip Van Petegem, Hamlet Abnousi   +6 more
doaj   +1 more source

Nanobody Therapeutics in Alzheimer’s Disease: From Molecular Mechanisms to Translational Approaches

Antibodies
Nanobodies (single-domain antibodies, VHHs) have emerged as versatile tools for evaluating and treating Alzheimer’s disease (AD). They offer distinct engineering benefits compared with traditional antibodies and small molecules, including small size ...
Deepika Godugu, Kranthi Gattu, Parul Suri, Abel B. Daartey, Krishna Jadhav, Satish Rojekar   +5 more
doaj   +1 more source

Evaluation of antidiphtheria toxin nanobodies

Nanotechnology, Science and Applications, 2010
Nanobodies are the smallest fragments of naturally occurring single-domain antibodies that have evolved to be fully functional in the absence of a light chain. Conventional antibodies are glycoproteins comprising two heavy and two light chains. Surprisingly, all members of the Camelidae family possess a fraction of antibodies devoid of both light ...
openaire   +4 more sources

Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins

, 2017
The study of membrane protein function and structure requires their successful detection, expression, solubilization, and/or reconstitution, which poses a challenging task and relies on the availability of suitable tools.
Chávez-Gutiérrez, L., De Strooper, Bart, Veugelen, Sarah, M. Dewilde, S. Veugelen, B. De Strooper, Dewilde, Maarten, L. Chávez-Gutiérrez   +7 more
core   +1 more source