Results 61 to 70 of about 14,448 (252)

Structural Basis of Nanobodies Targeting the Prototype Norovirus

, 2019
Human norovirus infections are a major disease burden. In this study, we analyzed three new norovirus-specific Nanobodies that interacted with the prototype human norovirus (i.e., GI.1). We showed that the Nanobodies bound on the side (Nano-7 and Nano-62)
Koromyslova, A, Ruoff, K, Roggenbach, I, Devant, J, Hansman, G, Geiss, C, Hempelmann, A, Haas, M, Ringel, A, Graf, J, Kilic, T   +10 more
core   +1 more source

Nanobodies detecting and modulating GPCRs outside in and inside out [PDF]

, 2019
G protein-coupled receptors (GPCRs), belonging to the largest class of membrane proteins, play a prominent role in many (patho)physiological processes and are, therefore, important drug targets.
Smit, Martine J., De Groof, Timo W.M., Smit, Martine J.; id_orcid, Heukers, Raimond   +3 more
core   +1 more source

Structural characterization of two nanobodies targeting the ligand-binding pocket of human Arc.

PLoS ONE
The activity-regulated cytoskeleton-associated protein (Arc) is a complex regulator of synaptic plasticity in glutamatergic neurons. Understanding its molecular function is key to elucidate the neurobiology of memory and learning, stress regulation, and ...
José M Godoy Muñoz, Lasse Neset, Sigurbjörn Markússon, Sarah Weber, Oda C Krokengen, Aleksi Sutinen, Eleni Christakou, Andrea J Lopez, Clive R Bramham, Petri Kursula   +9 more
doaj   +1 more source

Uniform Orientation of Biotinylated Nanobody as an Affinity Binder for Detection of Bacillus thuringiensis (Bt) Cry1Ac Toxin

Toxins, 2014
Nanobodies are the smallest natural fragments with useful properties such as high affinity, distinct paratope and high stability, which make them an ideal tool for detecting target antigens.
Min Li, Min Zhu, Cunzheng Zhang, Xianjin Liu, Yakun Wan   +4 more
doaj   +1 more source

The Development and Pilot Clinical Study of CD147 Targeted Antagonistic Peptide Probe for Tumor Imaging

Advanced Science, EarlyView.
This study establishes [68Ga]Ga‐DOTA‐AP9 as a first‐in‐human CD147‐targeted PET tracer with favorable safety and specific tumor uptake. Tracer accumulation correlates with CD147 expression in patients, enabling noninvasive quantification of CD147‐positive malignancies.
Xiaokun Ma, Rui Guo, Dongfeng Niu, Yufei Song, Zhenyao Zhang, Xiangxi Meng, Lili Mao, Haifeng Huang, Hua Zhu, Zhi Yang, Teli Liu   +10 more
wiley   +1 more source

Nanobody-Displaying Flagellar Nanotubes [PDF]

Scientific Reports, 2018
AbstractIn this work we addressed the problem how to fabricate self-assembling tubular nanostructures displaying target recognition functionalities. Bacterial flagellar filaments, composed of thousands of flagellin subunits, were used as scaffolds to display single-domain antibodies (nanobodies) on their surface.
Ágnes Klein, Mátyás Kovács, Adél Muskotál, Hajnalka Jankovics, Balázs Tóth, Mihály Pósfai, Ferenc Vonderviszt   +6 more
openaire   +3 more sources

Evolution of nanobodies specific for BCL11A

Proceedings of the National Academy of Sciences, 2023
Transcription factors (TFs) control numerous genes that are directly relevant to many human disorders. However, developing specific reagents targeting TFs within intact cells is challenging due to the presence of highly disordered regions within these proteins.
Maolu Yin, Manizheh Izadi, Karin Tenglin, Thibault Viennet, Liting Zhai, Ge Zheng, Haribabu Arthanari, Laura M. K. Dassama, Stuart H. Orkin   +8 more
openaire   +2 more sources

FUCA2 Sustains AKT Signaling and Suppresses Senescence by Antagonizing FUT3‐Mediated ErbB3 Fucosylation in Lung Adenocarcinoma

Advanced Science, EarlyView.
ABSTRACT While targeted therapies have improved outcomes in lung adenocarcinoma (LUAD), many patients still lack targetable mutations. Here, we identified alpha‐L‐fucosidase 2 (FUCA2) as a crucial driver of LUAD by preventing cellular senescence. Mechanistically, through the restriction of fucosyltransferase 3 (FUT3)‐mediated α‐1,3‐fucosylation of ...
Lu Chen, Jintao Guo, Fei Li, Mingjie Gao, Runyang Li, Zhaozhang Huang, Yaolin Zheng, Chunyi Gao, Jihuan Hou, Qiang Yu, Bowen Zheng, Xuemei Chen, Wenqing Zhang, Xiaoting Hong, Yali Zheng, Daxuan Wang, Qiyuan Li, Tianhui Hu, Yan‐yan Zhan   +18 more
wiley   +1 more source

Chelator‐Free Radiometal Labeling Inside Engineered Affibodies

Angewandte Chemie, EarlyView.
Affibodies are compact and robust three‑helix binders used in imaging and therapy. Engineering a triple‑cysteine motif into their core enables chelator‑free binding of important radiometals, like Bi(III), Pb(II), In(III), and Ga(III) with rapid uptake and exceptional stability.
Lani J. Davies, Upamali Somathilake, Santhanalaxmi Kumaresan, Frank Bruchertseifer, Alfred Morgenstern, Sarah Spreckelmeyer, Christoph Nitsche   +6 more
wiley   +2 more sources

Iterative In Silico Screening for Optimizing Stable Conformation of Anti-SARS-CoV-2 Nanobodies

Pharmaceuticals
Nanobodies (Nbs or VHHs) are single-domain antibodies (sdAbs) derived from camelid heavy-chain antibodies. Nbs have special and unique characteristics, such as small size, good tissue penetration, and cost-effective production, making Nbs a good ...
Wenyuan Shang, Xiujun Hu, Xiaoman Lin, Shangru Li, Shuchang Xiong, Bingding Huang, Xin Wang   +6 more
doaj   +1 more source