Results 51 to 60 of about 19,910 (273)

Recombinant expression of nanobodies and nanobody-derived immunoreagents

Protein Expression and Purification, 2020
Antibody fragments for which the sequence is available are suitable for straightforward engineering and expression in both eukaryotic and prokaryotic systems. When produced as fusions with convenient tags, they become reagents which pair their selective binding capacity to an orthogonal function.
openaire   +3 more sources

Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics [PDF]

Frontiers in Immunology, 2017
Monoclonal antibodies have revolutionized cancer therapy. However, delivery to tumor cells in vivo is hampered by the large size (150 kDa) of conventional antibodies. The minimal target recognition module of a conventional antibody is composed of two non-covalently associated variable domains (VH and VL).
Peter Bannas, Julia Hambach, Friedrich Koch-Nolte   +2 more
openaire   +3 more sources

Improved detection of molecular markers of atherosclerotic plaques using sub-millimeter PET imaging [PDF]

, 2020
Since atherosclerotic plaques are small and sparse, their non-invasive detection via PET imaging requires both highly specific radiotracers as well as imaging systems with high sensitivity and resolution.
Bormans, Guy, Bridoux, Jessica, Broisat, Alexis, Caveliers, Vicky, Cleeren, Frederik, Debie, Pieterjan, Descamps, Benedicte, Devoogdt, Nick, Hernot, Sophie, Neyt, Sara, Vanhove, Christian, Xavier, Catarina   +11 more
core   +2 more sources

Nanobody‐Conjugated Theranostic Prodrug Targeting αvβ3 Integrin Enables Precision Cancer Therapy With Real‐Time Imaging

Advanced Healthcare Materials, EarlyView.
The image shows a schematic form a nanobody‐conjugated theranostic prodrug (NBD) platform targeting tumor‐associated αvβ3 integrin. The NBD system integrates selective nanobody‐mediated tumor recognition, glutathione‐responsive disulfide cleavage for doxorubicin release, and aza‐BODIPY‐based near‐infrared fluorescence for real‐time imaging.
Sanu Karan, Hyunseung Lee, Rema Naskar, Mi Young Cho, Hye Sun Park, Eun Hee Han, Soumyadip Hore, Sun Hee Cho, Truc Ngoc Kim Lam, Taewoong Son, Tae Jung Park, Inkyu Hwang, Jonathan L. Sessler, Kwan Soo Hong   +13 more
wiley   +1 more source

Nanobodies in cell-mediated immunotherapy: On the road to fight cancer

Frontiers in Immunology, 2023
The immune system is essential in recognizing and eliminating tumor cells. The unique characteristics of the tumor microenvironment (TME), such as heterogeneity, reduced blood flow, hypoxia, and acidity, can reduce the efficacy of cell-mediated immunity.
Amirhosein Maali, Amirhosein Maali, Monireh Gholizadeh, Monireh Gholizadeh, Saba Feghhi-Najafabadi, Ahmad Noei, Seyedeh Sheila Seyed-Motahari, Seyedeh Sheila Seyed-Motahari, Shafieeh Mansoori, Zahra Sharifzadeh   +9 more
doaj   +1 more source

Structure of a bacterial type IV secretion core complex at subnanometre resolution [PDF]

, 2013
Type IV secretion (T4S) systems are able to transport DNAs and/or proteins through the membranes of bacteria. They form large multiprotein complexes consisting of 12 proteins termed VirB1-11 and VirD4.
Chandran, Vidya, Fronzes, Remi, Laeremans, T., Lian, Pei W., Orlova, Elena, Pardon, E., Remaut, Han, Rivera-Calzada, A., Savva, Christos G., Steyaert, J., Waksman, Gabriel   +10 more
core   +1 more source

Plant‐Produced Viral Nanoparticles Decorated with Nanobodies Against HER2 Improve Retention and Recruitment of Immune Cells in Solid Tumors

Advanced Healthcare Materials, EarlyView.
Potato virus X is a filamentous RNA plant virus that can be engineered into a molecular tool for cancer therapy. We produced genetically‐encoded virus‐derived nanoparticles decorated with nanobodies targeting cancer cell receptors, epidermal growth factor receptor (EGFR) and human epidermal growth factor 2 (HER2).
Enrique Lozano‐Sanchez, Miguel A. Moreno‐Gonzalez, Fernando Merwaiss, Nicole F. Steinmetz, José‐Antonio Daròs   +4 more
wiley   +1 more source

Nanobody technology : a versatile toolkit for microscopic imaging, protein-protein interaction analysis, and protein function exploration [PDF]

, 2017
Over the last two decades, nanobodies or single-domain antibodies have found their way in research, diagnostics, and therapy. These antigen-binding fragments, derived from Camelid heavy chain only antibodies, possess remarkable characteristics that favor
Beghein, Els, Gettemans, Jan
core   +3 more sources

Unlocking the Potential of MXene‐Based Electrochemical Biosensors: A Review of Biofunctionalization Strategies and Biosensing Principles

Advanced Materials Technologies, EarlyView.
ABSTRACT Electrochemical biosensors enable the accurate and timely detection of clinical biomarkers, improving healthcare and precision medicine. MXene nanosheets, a class of 2D transition metal carbides, nitrides, and carbonitrides, are promising materials for developing next‐generation electrochemical biosensors due to their unique physicochemical ...
Muhsin Ali, Sharat Chandra Barman, Isobel Steer, Aya H. Alshareef, Dana Alsulaiman   +4 more
wiley   +1 more source

Visual and High-Efficiency Secretion of SARS-CoV-2 Nanobodies with Escherichia coli

Biomolecules
Nanobodies have gained attention as potential therapeutic and diagnostic agents for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) due to their ability to bind and neutralize the virus.
Shuai Zhao, Wanting Zeng, Fang Yu, Pingping Xu, Chin-Yu Chen, Wanping Chen, Yanming Dong, Fei Wang, Lixin Ma   +8 more
doaj   +1 more source