Results 131 to 140 of about 14,448 (252)

Performance of pyDock in 8th CAPRI: Energy‐Based Scoring Applied to Docking and AlphaFold Models

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The 8th CAPRI edition has shown a significant evolution in the field of protein–protein complex structure prediction. We have participated in all 11 targets proposed in this edition, involving domain‐domain, protein–protein, protein‐peptide, and protein‐DNA interactions, including homo‐ and hetero‐meric interfaces.
Luis Angel Rodríguez‐Lumbreras, Mireia Rosell, Miguel Romero‐Durana, Víctor Monteagudo, Fabian Glaser, Juan Fernandez‐Recio   +5 more
wiley   +1 more source

Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins

, 2017
The study of membrane protein function and structure requires their successful detection, expression, solubilization, and/or reconstitution, which poses a challenging task and relies on the availability of suitable tools.
Chávez-Gutiérrez, L., De Strooper, Bart, Veugelen, Sarah, M. Dewilde, S. Veugelen, B. De Strooper, Dewilde, Maarten, L. Chávez-Gutiérrez   +7 more
core   +1 more source

A Versatile Multiplexed Immunofluorescence Strategy for Efficient, Host‐Independent, and Scalable Spatial Protein Profiling

Small Methods, EarlyView.
Crowding‐enhanced multiplex immunofluorescence (umIF) provides a versatile strategy for scalable spatial profiling in FFPE tissues. By improving labeling efficiency across direct, one‐step, and two‐step IF formats, umIF enables host‐species‐independent use of primary antibodies and antibody–nanobody complexes, detection of weak targets, and multicycle ...
Phuong Nguyen, Hongqiang Ma, Dimitrios S. Gotsis, Maomao Chen, Chaojie Zhang, Tushar Talukder Showrav, Marc Schwartz, Brenda Diergaarde, Robert E. Schoen, Rhonda M. Brand, Hua Zhang, Yang Liu   +11 more
wiley   +1 more source

Recent Advances in Photocatalyst‐Driven Protein Labeling and Proximity Mapping

The Chemical Record, EarlyView.
Photocatalyst‐driven protein labeling and proximity mapping have rapidly advanced as powerful strategies for spatiotemporal control in complex biological environments. This review integrates recent developments across single‐electron transfer and energy transfer‐based mechanisms and highlights how catalyst design, reactive intermediates, and diffusion ...
Shinichi Sato, Kazuki Miura, Juki Nakao, Hiroyuki Nakamura   +3 more
wiley   +1 more source

Neutralizing Nanobodies targeting diverse chemokines effectively inhibit chemokine function

, 2013
Background: Chemokines play a prominent role in inflammatory diseases. Results: Nanobodies targeting chemokines display high affinity and potently neutralize chemokine-induced receptor binding and signaling.
Bosch, L., Haard de, H., Smit, M.J.; id_orcid, Smit, M.J., Blanchetot, C., Mujic-Delic, A., Verrips, C.T., Saunders, M., Rem, L., Verzijl, D., Leurs, R.   +10 more
core   +1 more source

Nanobody Therapeutics in Alzheimer’s Disease: From Molecular Mechanisms to Translational Approaches

Antibodies
Nanobodies (single-domain antibodies, VHHs) have emerged as versatile tools for evaluating and treating Alzheimer’s disease (AD). They offer distinct engineering benefits compared with traditional antibodies and small molecules, including small size ...
Deepika Godugu, Kranthi Gattu, Parul Suri, Abel B. Daartey, Krishna Jadhav, Satish Rojekar   +5 more
doaj   +1 more source

Diagnostic antibodies at an inflection point: From discovery platforms to design‐oriented engineering

VIEW, EarlyView.
This study systematically organizes the evolution of diagnostic antibodies from discovery platforms to design‐oriented engineering and analyzes the strengths and limitations of each approach. Meanwhile, it provides a critical, literature‐driven synthesis of current challenges, proposing integrated strategies for assay‐aware and function‐driven antibody
Qitao Song, Lulai Xu, Weilin Wu, Fei Xie, Yantong Pan, Tiantian Yang, Xiaoxia Xu, Yanyin Lin, Lihua Zhang, Jiahui Zhou, Junyou Zhou, Linping Li, Zhanhui Wang, Weijing Yi, Jing Wang   +14 more
wiley   +1 more source

Modulating ion channels with nanobodies

Synthetic and Systems Biotechnology
Ion channels play instrumental roles in regulating membrane potential and cross-membrane signal transduction, thus making them attractive targets for understanding various physiological processes and associated diseases. Gaining a deeper understanding of their structural and functional properties has significant implications for developing therapeutic ...
Sher Ali, Ashley Suris, Yun Huang, Yubin Zhou   +3 more
openaire   +3 more sources

Peptide‐directed nanovehicles enabling tumor‐selective ROS amplification and antitumor immunity

VIEW, EarlyView.
Cancer cells are vulnerable to oxidative stress, yet exploit immune tolerance to escape destruction. In contrast, their metabolic profile is markedly altered to support rapid proliferation, accompanied by elevated production of reactive oxygen species (ROS).
Manuela Calin, Galyna Bila, Ileana Manduteanu, Rostyslav Bilyy   +3 more
wiley   +1 more source

Nanobodies - the new concept in antibody engineering

, 2010
Nanobodies are antibody-derived therapeutic proteins that contain the unique structural and functional properties of naturally occurring heavy-chain antibodies.
Wang, X, Li, L, Deffar, K, Shi, H, Zhu, X   +4 more
core   +1 more source