Results 81 to 90 of about 12,492 (212)
Production of a mono-biotinylated EGFR nanobody in the E. coli periplasm using the pET22b vector
BMC Research Notes, 2018 Objective Our aim was to produce a mono-biotinylated single domain antibody (‘nanobody’) specific for the epidermal growth factor receptor (EGFR), which is overexpressed in many cancer cells.
Alfiah Noor +7 more
doaj +1 more source
Fluorescent Diarylethenes With Polar Groups: Synthesis, Spectra, and Optical Microscopy Applications
Chemistry – A European Journal, EarlyView.Blinking green and flashing red: photo‐switchable and photo‐activatable probes emitting green and red light were prepared and applied as bioconjugates in super‐resolution optical microscopy. ABSTRACT The use of photoactivatable fluorescent diarylethenes (fDAEs) in biology‐related light microscopy has been restricted by the lack of probes having freely ...
Kakishi Uno +6 more
wiley +1 more source
Preparation of a Nanobody Specific to Dectin 1 and Its Anti-inflammatory Effects on Fungal Keratitis
International Journal of Nanomedicine, 2022 Xing Liu,1 Jianxin Sui,2 Cui Li,1 Xudong Peng,1 Qian Wang,1 Nan Jiang,1 Qiang Xu,1 Luokai Wang,2 Jing Lin,1 Guiqiu Zhao1 1Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, People’s Republic of China; 2College of Food ...
Liu X +9 more
doaj
Molecules, 2019 Despite the advantages of the nanobody, the unique structure limits its use in sandwich immunoassay. In this study, a facile protocol of sandwich immunoassay using the nanobody was established.
Li Xu +3 more
doaj +1 more source
An Orthogonal Way of Writing the Tubulin Code
Cytoskeleton, EarlyView.ABSTRACT The Elongator complex has long been characterized for its role in tRNA modification and modulation of protein translation. Beyond these established functions, recent findings reveal an unexpected role for Elongator in directly interacting and tuning microtubule dynamics and properties, broadening its biological significance in eukaryotic cells.
Vicente Jose Planelles‐Herrero +1 more
wiley +1 more source
Nanobody-based bispecific T-cell engager (Nb-BiTE): a new platform for enhanced T-cell immunotherapy
Signal Transduction and Targeted Therapy, 2023 Xiao-mei Yang +9 more
doaj +1 more source
A Novel Anti-CD47 Nanobody Tetramer for Cancer Therapy
AntibodiesCD47 acts as a defense mechanism for tumor cells by sending a “don’t eat me” signal via its bond with SIRPα. With CD47’s overexpression linked to poor cancer outcomes, its pathway has become a target in cancer immunotherapy.
Nataliya M. Ratnikova +5 more
doaj +1 more source
Cancer Nexus, EarlyView.ABSTRACT Lipid nanoparticles (LNPs) represent the most clinically advanced platform for RNA delivery and have enabled major breakthroughs in vaccines and gene therapies. However, their broader application is still limited by inefficient extrahepatic delivery, immunogenicity, and insufficient control over tissue‐ and cell‐specific targeting. This review
Yu Han +5 more
wiley +1 more source
Cytometry Part B: Clinical Cytometry, EarlyView.Abstract Accurate quantification of chimeric antigen receptor (CAR) T cells is essential for monitoring post‐infusion CART expansion and persistence and for real‐time clinical decision‐making. Multiparameter flow cytometry (MFC) enables rapid, live‐cell detection with absolute quantification and concurrent immunophenotypic characterization. This review
Jianhua Ling, Wei Wang, Sa A. Wang
wiley +1 more source
Food Safety and Health, EarlyView.A sensitive colloidal gold immunochromatographic assay for atrazine was successfully established using genetically engineered nanobody‐recombinant antibody, which overcame the shortcomings of traditional antigen‐antibody. ABSTRACT Colloidal gold immunochromatography (CG‐ICA) is distinguished by its cost‐effectiveness and rapid detection capabilities ...
Jiayong Bao +16 more
wiley +1 more source