Results 81 to 90 of about 16,593 (260)

Evaluation of the yeast surface display system for screening of functional nanobodies

AMB Express, 2020
Yeast surface display is a powerful technology used to isolate and engineer proteins to improve their activity, specificity, and stability. In this method, gene expression is regulated by promoters, and secretion efficiency is affected by secretion ...
Kaho Kajiwara, Wataru Aoki, Mitsuyoshi Ueda   +2 more
doaj   +1 more source

Cortactin and fascin-1 regulate extracellular vesicle release by controlling endosomal trafficking or invadopodia formation and function [PDF]

, 2018
Cancer cell-derived extracellular vesicles (EVs) are increasingly being recognized as genuine invasive structures as they contribute to many aspects of invasion and metastasis.
Beghein, Els, Devriese, Delphine, Gettemans, Jan, Van Hoey, Evy   +3 more
core   +2 more sources

The Therapeutic Potential of Nanobodies [PDF]

BioDrugs, 2019
Today, bio-medical efforts are entering the subcellular level, which is witnessed with the fast-developing fields of nanomedicine, nanodiagnostics and nanotherapy in conjunction with the implementation of nanoparticles for disease prevention, diagnosis, therapy and follow-up.
Ivana Jovčevska, Serge Muyldermans
openaire   +2 more sources

DNA Nanostructure‐Templated Multivalency Enables Broad‐Spectrum Virus Inhibition

Advanced Science, EarlyView.
A honeycomb‐shaped DNA nanostructure is introduced that organizes nanobodies or aptamers into trimeric clusters mirroring the native hemagglutinin arrangement on influenza viruses. This geometry‐matched multivalency enhances binding avidity by up to 1000‐fold, resulting in robust, broad‐spectrum viral neutralization and a 30–55% improvement in host ...
Saurabh Umrao, Abhisek Dwivedy, Dhanush Gandavadi, Chi Chen, Lifeng Zhou, Jinwei Duan, Vineetha Mareddy, Ying Fang, Xing Wang   +8 more
wiley   +1 more source

An Expanded Toolbox for Versatile Chemical Editing of Adeno‐Associated Virus

Angewandte Chemie, EarlyView.
We describe technology to introduce diverse non‐natural chemical functionalities site‐specifically into the capsid of adeno‐associated virus through genetic code expansion, and using them to engineer this leading vector for human gene therapy for enhanced tissue specificity and reduced immunogenicity Abstract Site‐specific incorporation of noncanonical
Quan Pham, Jake Glicksman, Boyang Han, David Koo, Conor Loynd, Soumya Jyoti Singha Roy, Abhishek Chatterjee   +6 more
wiley   +2 more sources

Gi- and Gs-coupled GPCRs show different modes of G-protein binding. [PDF]

, 2018
More than two decades ago, the activation mechanism for the membrane-bound photoreceptor and prototypical G protein-coupled receptor (GPCR) rhodopsin was uncovered.
Altenbach, Christian, Caro, Lydia N, Dror, Ron O, Ernst, Oliver P, Hollingsworth, Scott A, Hubbell, Wayne L, Latorraca, Naomi R, Van Eps, Ned   +7 more
core   +1 more source

NANOBODIES(®): A Review of Generation, Diagnostics and Therapeutics

, 2023
NANOBODY(®) (a registered trademark of Ablynx N.V) molecules (Nbs), also referred to as single domain-based VHHs, are antibody fragments derived from heavy-chain only IgG antibodies found in the Camelidae family. Due to their small size, simple structure, high antigen binding affinity, and remarkable stability in extreme conditions, nanobodies possess ...
Jin, Bo-Kyung, Odongo, Steven, Radwanska, Magdalena, Stefan, Magez   +3 more
openaire   +2 more sources

Ionic–Bionic Interfaces: Advancing Iontronic Strategies for Bioelectronic Sensing and Therapy

Advanced Science, EarlyView.
Ionic–bionic interfaces for bioelectronics leverage ions as multifunctional mediators that combine mechanical compliance, ionic and electronic functionalities, and therapeutic effects. These systems offer real‐time biosignal transduction, effective wound dressing, responsive drug delivery, and seamless interaction between soft tissues and electronic ...
Yun Goo Ro, Yoojin Chang, Jeeyoon Kim, Seungjae Lee, Sangyun Na, Cheolhong Park, Hyunhyub Ko   +6 more
wiley   +1 more source

Unnatural Amino Acid and Emerging Chemistry Approaches to Map RNA–Protein Interactions

Angewandte Chemie, EarlyView.
This review highlights emerging chemistries for mapping RNA–protein interactions, including genetically encoded unnatural amino acids, novel photocrosslinkers, and non‐photoactivatable crosslinking systems. We compare their mechanisms, reactivity and applications, outlining how these next‐generation tools enable higher‐resolution, site‐specific ...
Eryn Lundrigan, Parrish Evers, Elexa Scott, John Paul Pezacki   +3 more
wiley   +2 more sources

Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies

Scientific Reports, 2021
Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed peptide barcoding,
Yusei Matsuzaki, Wataru Aoki, Takumi Miyazaki, Shunsuke Aburaya, Yuta Ohtani, Kaho Kajiwara, Naoki Koike, Hiroyoshi Minakuchi, Natsuko Miura, Tetsuya Kadonosono, Mitsuyoshi Ueda   +10 more
doaj   +1 more source