Results 1 to 10 of about 65,196 (347)

Rolling circle reverse transcription enables high fidelity nanopore sequencing of small RNA.

PLoS ONE, 2022
Small RNAs (sRNAs) are an important group of non-coding RNAs that have great potential as diagnostic and prognostic biomarkers for treatment of a wide variety of diseases. The portability and affordability of nanopore sequencing technology makes it ideal
Sean Maguire, Shengxi Guan
doaj   +2 more sources

Forensic SNP genotyping using nanopore MinION sequencing [PDF]

, 2017
One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using
Cornelis, Senne, Deforce, Dieter, Deleye, Lieselot, Gansemans, Yannick, Van Nieuwerburgh, Filip   +4 more
core   +2 more sources

Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome

Genome Medicine, 2019
Background Intrachromosomal triplications (TRP) can contribute to disease etiology via gene dosage effects, gene disruption, position effects, or fusion gene formation.
Claudia M. B. Carvalho, Zeynep Coban-Akdemir, Hadia Hijazi, Bo Yuan, Matthew Pendleton, Eoghan Harrington, John Beaulaurier, Sissel Juul, Daniel J. Turner, Rupa S. Kanchi, Shalini N. Jhangiani, Donna M. Muzny, Richard A. Gibbs, Baylor-Hopkins Center for Mendelian Genomics, Pawel Stankiewicz, John W. Belmont, Chad A. Shaw, Sau Wai Cheung, Neil A. Hanchard, V. Reid Sutton, Patricia I. Bader, James R. Lupski   +21 more
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Nanopore-based metagenomic sequencing for the rapid and precise detection of pathogens among immunocompromised cancer patients with suspected infections

Frontiers in Cellular and Infection Microbiology, 2022
Cancer patients are at high risk of infections and infection-related mortality; thereby, prompt diagnosis and precise anti-infectives treatment are critical.
Qingmei Deng, Qingmei Deng, Qingmei Deng, Yongqing Cao, Xiaofeng Wan, Xiaofeng Wan, Bin Wang, Aimin Sun, Aimin Sun, Huanzhong Wang, Huanzhong Wang, Yunfei Wang, Hongzhi Wang, Hongzhi Wang, Hongzhi Wang, Hongcang Gu, Hongcang Gu, Hongcang Gu   +17 more
doaj   +1 more source

DNA methylation-calling tools for Oxford Nanopore sequencing: a survey and human epigenome-wide evaluation

Genome Biology, 2021
Background Nanopore long-read sequencing technology greatly expands the capacity of long-range, single-molecule DNA-modification detection. A growing number of analytical tools have been developed to detect DNA methylation from nanopore sequencing reads.
Yang Liu, Wojciech Rosikiewicz, Ziwei Pan, Nathaniel Jillette, Ping Wang, Aziz Taghbalout, Jonathan Foox, Christopher Mason, Martin Carroll, Albert Cheng, Sheng Li   +10 more
doaj   +1 more source

MinION Analysis and Reference Consortium: Phase 1 data release and analysis [PDF]

, 2015
The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools.
Benedict Paten, Bonnie L. Brown, Camilla L.C. Ip, David A. Eccles, David Buck, Elizabeth M. Batty, Ewan Birney, Hans J. Jansen, Hugh E. Olsen, Jared T. Simpson, John M. Urban, John R. Tyson, Justin O'Grady, Mariateresa de Cesare, Matthew Loose, MinION Analysis and Reference Consortium, Miten Jain, Paolo Piazza, Richard M. Leggett, Rory J. Bowden, Sara Goodwin, Solomon Mwaigwisya, Terrance P. Snutch, Vadim Zalunin   +23 more
core   +2 more sources

Simulation of Nanopore Sequencing Signals Based on BiGRU

Sensors, 2020
Oxford Nanopore sequencing is an important sequencing technology, which reads the nucleotide sequence by detecting the electrical current signal changes when DNA molecule is forced to pass through a biological nanopore.
Weigang Chen, Peng Zhang, Lifu Song, Jinsheng Yang, Changcai Han   +4 more
doaj   +1 more source

A Hybrid Sequencing Approach Completes the Genome Sequence of Thermoanaerobacter ethanolicus JW 200 [PDF]

, 2019
Thermoanaerobacter ethanolicus JW 200 has been identified as a potential sustainable biofuel producer due to its ability to readily ferment carbohydrates to ethanol. A hybrid sequencing approach, combining Oxford Nanopore and Illumina DNA sequence reads,
Ayine, Monica L., Gill, Steven R., Gilman, James A., Hewlett, Mark, James, Paul B.C., Love, John, Parker, David A., Power, Ann L., Singleton, Chloe, Tennant, Richard K.   +9 more
core   +1 more source

Metagenomic identification of severe pneumonia pathogens in mechanically-ventilated patients: a feasibility and clinical validity study

Respiratory Research, 2019
Background Metagenomic sequencing of respiratory microbial communities for pathogen identification in pneumonia may help overcome the limitations of culture-based methods. We examined the feasibility and clinical validity of rapid-turnaround metagenomics
Libing Yang, Ghady Haidar, Haris Zia, Rachel Nettles, Shulin Qin, Xiaohong Wang, Faraaz Shah, Sarah F. Rapport, Themoula Charalampous, Barbara Methé, Adam Fitch, Alison Morris, Bryan J. McVerry, Justin O’Grady, Georgios D. Kitsios   +14 more
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Application of nanopore adaptive sequencing in pathogen detection of a patient with Chlamydia psittaci infection

Frontiers in Cellular and Infection Microbiology, 2023
IntroductionNanopore sequencing has been widely used in clinical metagenomic sequencing for pathogen detection with high portability and real-time sequencing.
Yanfeng Lin, Yanfeng Lin, Yan Dai, Shuang Zhang, Shuang Zhang, Hao Guo, Lang Yang, Jinhui Li, Kaiying Wang, Ming Ni, Ming Ni, Zongqian Hu, Zongqian Hu, Leili Jia, Huiying Liu, Peng Li, Hongbin Song, Hongbin Song   +17 more
doaj   +1 more source